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Effects of gamma and electron beam irradiation on viability and DNA elimination of Staphylococcus aureus

Effects of gamma and electron beam irradiation on viability and DNA elimination of Staphylococcus aureus

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Published by iMedPub
Owing to the inability of real-time polymerase chain reaction (PCR)
to distinguish between signals originating from viable cells and DNA released from
dead cells, (i.e. it can detect dead cells) a risk exists that DNA from dead cells can result
in false-positive PCR signals, so it is important to use a proper method for sterilization
to maintain the accuracy of the quantitative real-time PCR assay.
Owing to the inability of real-time polymerase chain reaction (PCR)
to distinguish between signals originating from viable cells and DNA released from
dead cells, (i.e. it can detect dead cells) a risk exists that DNA from dead cells can result
in false-positive PCR signals, so it is important to use a proper method for sterilization
to maintain the accuracy of the quantitative real-time PCR assay.

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Categories:Types, Research
Published by: iMedPub on Dec 11, 2011
Copyright:Traditional Copyright: All rights reserved
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12/27/2013

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