RLPBR1 BF LRlNL 1LS1

Fulfilling Tasks in the Context of Class XI Subject Biology Semestei II

Acauemic Yeai













TEACHER :
Mrs. ANIK SILVI W.
By :
SITI SHOFIYA NOVITA SARI ( XI SCIENCE 1 / 28 )

SMA NEGERI 1 BO1ONEGORO
2010/2011

A. PURPOSE :
To check the existence oI Albumin and Glucose Levels in the urine.

B. TOOLS AND MATERIALS
1. Urine 2. Biuret








3. Fehling A and B (Benedict) 4. Measuring Cup






5. Test Tube 6 . Pipette






. Bunsen 8. Clamp







9. Matches







C. METHODS
1. Albumin Test
O Prepare the materials and tools
O Put urine slowly into measuring cup until it reaches the height oI 2 cm.
Use a pipette to take an urine drops.







O Used as drops oI Biuret 5 drops

O Shake and observe color change. II the urine contained albumin, the color
oI urine will turn purple







BeIore AIter
2. Glucose Test
Enter the urine into a test tube, as high as 2 cm. Use the drop
pipe to take the urine








Used as drops oI Fehling solution A and B each 5 drops. Shake and
mix until evenly


Heat up the end oI the test tube over the Bunsen Ilame. heating
process must be intermittently liIted so as not to burn. Do not let boil.
Heating time 3-5 minutes









BeIore Burning AIter Burning








Observe the color change. II the urine contains glucose, urine color
will be red brick






BeIore AIter


URINE TEST RESULTS TABLE
Reagent
Urine
Original color Final Color
Biuret









Clear Yellow








Clear Yellowish
Fehling A and B
(Benedict)








Clear Yellow








Bluish Green

QUESTIONS :
1. Please list the characteristics of healthy urine!
O It has yellow clear color
O It doesn`t contain oI glucose or albumine
O It`s not muddy
O It`s not contain oI blood, because iI it contains blood it means that
something gets inIection in kandung kemih
. How to means whether if the urine contained albumin?
Albuminuria
. istinguish the color of urine before and after the mixed reagent!
a. Urine mixed with biuret reagent :
- BeIore : Clear Yellow
- AIter : Clear Yellowish (depend on the conditions oI urine)


b. Urine that have been mixed by reagent benedict (Fehling A and B)
- BeIore : Clear Yellow
- AIter : Bluish Green (depend on the conditions oI urine)

. What happens with urine if it`s positive diabetes?
AIter the drops with Fehling solution A and B, and burned, the urine is
yellow brown

. State the factors that influence the amount of urine!
O The amount oI water drunk
O Renal nerve stimulation that causes narrowing oI blood vessels leading
to glomerulus
O Antidioritic hormone (ADH)
O Concentration oI insuline hormone
O Levels oI salt that must be removed Irom the blood to keep the osmotic
pressure
O A person suIIering Irom diabetes mellitus (diabetes) glucose
expenditure Iollowed by the increase in urine volume


CONCLUSION
1. II urine is mixed with Biuret and changed into purple or ther`s ring oI
purple in surIace oI mixture, it means that mixture is positive to contain
oI albumine or protein. II that happens, the person positive get
Albuminuria. It`s negative to contain protein iI the color is clear yellow
2. II urine is mixed with Benedict and changed into red brick or Iirst color is
yellow orange, it means that mixture is positive to contain oI glucose. II
that happens, the person get Diabetics. It`s negative to contain protein iI
the color is clear blue
3. Urine is inIluenced oI some Iactors
O The amount oI water drunk
O Renal nerve stimulation that causes narrowing oI blood vessels
leading to glomerulus
O Antidioritic hormone (ADH)
O Concentration oI insuline hormone
O Levels oI salt that must be removed Irom the blood to keep the
osmotic pressure
O A person suIIering Irom diabetes mellitus (diabetes) glucose
expenditure Iollowed by the increase in urine volume

4. Some disease can be known by urine test















Thank you