Welcome to Scribd. Sign in or start your free trial to enjoy unlimited e-books, audiobooks & documents.Find out more
Download
Standard view
Full view
of .
Look up keyword
Like this
128Activity
0 of .
Results for:
No results containing your search query
P. 1
Design Experiment SPM Biology

Design Experiment SPM Biology

Ratings:

3.0

(1)
|Views: 19,403|Likes:
Published by Ema
Design Experiment for biology SPM.Really handy in studying for paper 3 - section B.Credit goes to my biology teacher for this text
Design Experiment for biology SPM.Really handy in studying for paper 3 - section B.Credit goes to my biology teacher for this text

More info:

Published by: Ema on Dec 22, 2011
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as DOCX, PDF, TXT or read online from Scribd
See more
See less

06/05/2015

pdf

text

original

 
Ema-Mel0dy 
spm’11
 
1.TO INVESTIGATE OF
DIFFERENT TYPE OF CELL TO THE FIXED SHAPED OF THE CELL
 
Problem statement
Does plant cell and animal cell have fixed shape?
Hypothesis
Plant cell has a fixed shape but not animal cell
Variables
MV : type of cell/plant cell and animal cellRV : fixed shape of cellCV : One drop of methylene blue solution and distilled water
Apparatus and materials
Glass slide,cover slips,forcep,knife,microscope,toothpick,white tile/cutting board,methylene bluesolution,iodine solution,iodine solution,filter paper,distilled water,onion scale leaf,cheek cell
Procedure
1.
 
A scale leaf from an onion bulb are obtained2.
 
By using a forcep .the inner surface of onion scale leaf is peeled off 3.
 
One drop of distilled water was placed in the middle of glass slide4.
 
With a needle ,the cover slip is dropped slowly at 45
0
to the glass slide so that no air bubblebeing trapped5.
 
A drop of iodine solution was dropped at one side of the cover slip6.
 
A filter paper was placed at the opposite end of the opposite end of the cover slip to allow thespreading of solution absorbing excess solution7.
 
The slide is observed under a light microscope using a low power objective lens then high powerobjective lens8.
 
The plant structure is then drawn and recorded by using a microscope9.
 
This experiment is repeated using a cheek cell10.
 
The mouth is rinsed before starting with experiment11.
 
By using a toothpick, the inner mouth were scrapped to get some cheek cell12.
 
Then the cheek cell was placed onto a glass side13.
 
A drop of methylene blue solution was added14.
 
Slowly the cover slips was dropped, then the filter paper were placed at one end of the coverslip for irrigation15.
 
This slide is then observe and the structure was recorded by using a microscope16.
 
All the results are tabulated in a table
Presentation of Data
Type of cell Structure of cell seen under microscopeMagnification : 10 x 40Plant cell/onion scale leaf Animal cell/cheek cell
 
Ema-Mel0dy 
spm’11
 
2.TO DETERMINE THE
CONCENTRATION OF SUCROSE SOLUTION WHICH IS ISOTONIC TO THE CELL SAPOF POTATO STRIPProblem statement
What is the concentration of the sucrose solution that will maintain the length of potato strip?
Hypothesis
As the sucrose solution reach certain concentration (isotonic to the cell sap),there is no changes in thelength of potato strip
Variables
MV : the concentration of the sucrose solutionRV : change in length of potato stripFV : initial length of potato strip
Apparatus & material
Cork borer, test tubes,stopwatch,ruler,potatoes,various concentration of sucrose solution,filter paper
Procedure:
1.
 
Six test tube are labeled P,Q,R,S,T and U2.
 
Test tube P is filled with 10ml distilled water, test tube Q is filled with 10ml sucrose solutionO.1M,test tube R is filled with 10ml sucrose solution 0.2M,test tube S is filled with 10ml sucrosesolution 0.3M,test tube T is filled with 10ml sucrose solution 0.4M and test tubes U is filled with10ml sucrose solution 0.5M3.
 
The cork borer is pushed into the potato and the potato strip is obtained by pushing it out of thecork borer using a glass rod4.
 
The potato strips are cut to the exact length of 5 cm.5.
 
One potato cylinder is placed in each labeled test tubes for 30 minutes.6.
 
After 30minutes,the potato strips are removed from the test tube and gently wiped with filterpaper7.
 
The final length of the potato strips are measured and record using a ruler8.
 
The final length of the potato strips are recorded in a result table9.
 
A graph pf the concentration of sucrose solution against the change in the length is plotted
Presentation
Test tube Concentration of sucrosesolution(M)LengthInitial(cm) Final(cm) Change inlength(cm)P 0.0 5Q 0.1 5R 0.2 5S 0.3 5T 0.4 5U 0.5 5
 
Ema-Mel0dy 
spm’11
 
3.TO INVESTIGATE
THE EFFECTS OF ALBUMEN CONCETRATION ON THE ENZYME PEPSIN REACTION
 
Problem statement
What is the effect of different albumen concentration on the rate of enzyme reaction?
Hypothesis
The higher the albumen concentration,the higher the rate of enzyme reaction
Variables
MV : the concentration of albumen solutionRV : rate of enzyme reactionCV : the volume of albumen solution
Apparatus and Materials
Albumen solution(1%,2%,3%,4%),1% pepsin solution, pipette/measuring cylinder,HCL,waterbath,thermometer,stopwatch
Procedure
1.
 
5ml of 1% albumen solution is poured into a test tube using a pipette.The test tube is labeled P.2.
 
1 ml of HCL acid is poured into the same test tube using another pipette3.
 
1 ml of 5% pepsin is poured into the same test tube using another pipette.The mixture is shakenwell.4.
 
The test tube is placed in the beaker containing 300 ml of water at 37
o
C.A thermometer isplaced in the beaker to check the temperature.5.
 
The stopwatch is started6.
 
The mixture is observed and the time taken for the solution to turn colourless is taken using astopwatch and recorded in a table.7.
 
Steps 1 to 6 are repeated twice to get an average result8.
 
Steps 1 to 7 are repeated,replacing the 1% albumen solution with 2%,3% and 4% albumensolution respectively.9.
 
All data are recorded in a table and a graph of the rate of enzme reaction against the albumenconcentrated is plotted
Presentation of data
Concentration of albumensolution(%)Time taken for the mixture to turn colourless(min)
The rate of enzymereaction (min
-1
)1 2 3 average1234

Activity (128)

You've already reviewed this. Edit your review.
hudd liked this
all mqwe liked this
Jia Li liked this
Intan Nur Dania liked this
KhavirnaManjaari liked this
Ling PeNny liked this
Qin Zi liked this
SheilaTan liked this
chooshuting liked this
NatalieYX liked this

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->