Take another sample (2
sample) from slightly deeper (~4 m) and test this too.
Table 1. Water physico-chemical parameters
sample (~1m) 2
sample (~4m)pHTemperature (
C)Dissolved oxygen (mg/l)Salinity (ppt)Secchi depth (m?)
Use the Secchi disc to fill in the last parameter in Table 1. Follow these instructions:
Record the depth at which the disc disappears. Slowly raise the disc and record thedepth of reappearance. The Secchi depth is the median of the two readings. Repeat 3times and use the overall mean value as the final Secchi depth.
Roll out 30m of transect tape along the railing/edge of the jetty/pontoon/breakwater.
Take the plankton net rope and feed it through the PVC pole. Standing at one end of thetransect tape, drop the net into the water like you were fishing. Now, slowly walk alongthe tape until the end, making sure that the opening of the net is about 1m below thesurface as you pull it along. Turn around and repeat. Do this 4 times until you havewalked 120m.
Carefully pull up the net and catch the bottle. Unscrew the bottle and pour the contentsinto your sample jar. Now, replace the bottle and rinse the inside of the net using theseawater-filled wash bottle (I suggest all this is done in the bucket so you do not lose anywater). Pour this water into the same sample jar, seal and label.
Lab workA. MaterialsPer group:
Disposable pipettesDissecting stereomicroscopesIdentification sheetsSedgewick rafter chamber
B. Procedure 1: Exploring plankton diversity
Shake your sample bottle and pipette 1ml or so into a petri or glass dish. Put this under adissecting stereomicroscope and try and identify what you see.2.
Take 1ml from the pre-prepared concentrated samples (shake them first) at the front of the lab and repeat as 1. Look at the samples from both sites – are there any obviousdifferences?