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Journal FINAL....

Journal FINAL....

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Published by fiena92
Anti-Inflammatory
Anti-Inflammatory

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Published by: fiena92 on Jan 03, 2012
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06/07/2013

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Anti-inflammatory effect of Acmella uliginosa on acuteinflammation activities in mice
Norsafinah M.T.
a
, Nurfarah Diyanah A.K.A.
a
, Munirah M.M.
a
, Nur Mahirah Liyana D.
a
 Wan Ahamad Azran W.S.
b
, Hui Ming O.
c
, Prof Mohd Roslan S.
c
 
a
Foundation in Science and Technology Royal College of Medicine Perak, Kolej MARA Kuala Nerang,06300 Kuala Nerang, Kedah Darul Aman
b
Department of Biology of UniKL Kolej MARA Kuala Nerang, 06300 Kuala Nerang, Kedah Darul Aman
c
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti PutraMalaysia, 43400 Serdang, Selangor, Malaysia
 ___________________________________________________________________________
Abstract:
 
Ethnopharmacological relevance
:
 Acmella uliginosa
(Sw.) Cass. is a medicinal herbaceousplant that is commonly used by the Malay community in Malaysia to relieve pain oftenassociated with mouth ulcers, toothache, sore throat, and stomach ache.
 Aim:
The study was carried out to investigate the anti-inflammatory effect of the methanolicextract of 
 Acmella uliginosa
(Sw.) Cass. flowers (MEAU) using carrageenan-induced pawedema test in mice.
Materials & methods
:
Chemical (methanol, aspirin, absolute ethanol, carrageenan). Theextract was given via oral administration at doses of 3, 10, 30 and 100mg/kg. A total of 36mice were used and the mice were divided into 6 groups with 6 mice in each group.
Results
:I
t was demonstrated that intraperitoneal administration of MEAU at a dose of 3, 10,30 and 100mg/kg produced significant dose-dependent inhibition of paw edema induced bycarrageenan.
Conclusion
:
The results from the present study appear to support the folkloric belief in themedicinal properties of 
 Acmella uliginosa
(Sw.) class which concluded that MEAU possessedanti-inflammatory effect against inflammation.
Abstrak:
erkaitan ethnopharmacological 
 
:
 Acmellauliginosa
(Sw.) Cass. adalah sejenis tanaman herbaubat yang umum digunakan oleh masyarakat Melayu di Malaysia untuk menghilangkan rasasakit di mana sering dikaitkan dengan radang mulut, sakit gigi, sakit tekak, dan sakit perut.
Objektif 
:
Kajian ini telah dilakukan untuk menyiasat anti-inflamasi dari ekstrak methanolAcmella uliginosa (MEAU) dengan menggunakan karaginan-ujian edema yang diinduksi padatapak kaki tikus.
Bahan-bahan dan kaedah
:
Bahan kimia (methanol, aspirin, etanol mutlak, karaginan).Ekstrak MEAU telah diberikan melalui kaedah oral pada dos 3, 10, 30, dan 100mg/kg.Sebanyak 36 ekor tikus telah digunakan dan tikus-tikus tersebut telah dibahagi kepada 6kumpulan dengan 6 ekor tikus dalam setiap kumpulan.
 
eputusan
:I
ni telah menunjukkan bahawa pemberian intraperitoneal MEAU pada dos 3, 10,30 dan 100mg/kg menghasilkan kebergantungan dos yang menghalang edema pada tapakkaki tikus dengan signifikan oleh karagenan.
onklusi 
:
Hasil dari kajian ini telah terbukti menyokong kepercayaan masyarakat bahawa
 Acmella uliginosa
(Sw.) terdapat ciri-ciri perubatan yang menyimpulkan MEAU mempunyaikesan anti-inflamasi terhadap keradangan.
I
ntroduction:
 Acmella uliginosa (Sw.) Cass.
I
s aperennial herbaceous plant belonging to the daisy family,Asteraceae, which is indigenous and widely distributed in the tropic sand sub-tropicsespecially in the West
I
ndies, Venezuela, Brazil, Africa,
I
ndonesia and Malaysia (Pandey et al.,2007).
I
n Peninsular Malaysia, it is popularly known as Subang Nenek or Butang Baju SitiFatimah.
I
t grows in a bun- dance as a naturalized weed on open hill sides and the rockyshores of rivers. When consumed, its flowers and leaves have a pungent taste thataccompanied by tingling and numbness.
I
n Malaysia, A. uliginosa has been generally used asa traditional herbal medicine for its analgesic and antispasmodic properties. The use of theflowers of A. uliginosa in particular,are more common than other parts of the plant, and arewidely used as a remedy for the relief of pain especially in mouth ulcers, toothache, sorethroat and stomach ache. The flowers and/or the leaves are crushed and its paste istopically applied to the affected areas caused by insectbites to all eviate itch, redness andswelling. Despite the widespread use of this plant in the Malays folk medicine, yet there isno scientific investigation reported to support its biological properties. Therefore, in thelight of its reported analgesic activity, the present study was carried out to evaluate theantinociceptive property of the methanolic extractof A. uliginosa flowers using bothchemical and thermal-induced nociception models in mice.
 
 2.
 
Methadology2.1.
Plant collection and identification
Flowers of 
 A. uligionosa
were freshly collected from the Kampung Janda Baik, Pahang,Malaysia in February 2009. The plant material was identified and authenticatedtaxonomically by Dr. Shamsul Khamis, Biodiversity Unit,
I
nstitude of Bioscience, UniversityPutra Malaysia. A voucher specimen of the collected sample was deposited in thedepartmental herbarium, as voucher, SK1780/09.
2.2.
Preparation of extract 
 
The fresh flowers (1 kg) were washed with distilled water to remove the dirt and soakedovernight in methanol (99.8%). Then, the material was sonicated and filtered. The filtratewas then concentrated by using large scale rotary evaporator and lyophilization to yieldapproximately 2% of a greenish black colored methanolic extract of 
 Acmella uliginosa
 (MEAU). The extract then was stored at -20C prior to experiments.
2.3.
D
rugs and chemicals
Methanol, aspirin (100 mg/kg), Tween 20, absolute ethanol (100%), acetylsalicylic acid (ASA)and lambda ()-carrageenan was purchased from Sigma Chemicals Co. (St. Louis, MO, USA).Acetylsalicylic acid (ASA) and lambda ()-carrageenan were dissolved in saline solution (0.9%NaCl). The methanolic extract of 
 A. uligionosa
flowers (MEAU) was dissolved in vehicle(absolute ethanol
:
Tween 20 in saline; 5
:
5; v/v) to the desired concentration just before use.MEAU in vehicle or vehicle alone (control) was administered orally with an orogastriccannula, 30 min prior to acute inflammatory testing. The vehicle used alone had no effectson the inflammatory responses in mice.
2.4.
 A
nimals
 
Experiments were conducted using adult male
I
CR mice (2535 g) purchased from the
I
nstitute for Medical Research, Kuala Lumpur. They were housed at 22±2 °C under a 12-hlight/ 12-h dark cycle and with access to food and water ad libitum. The animals wereacclimatized and habituated to the laboratory for at least a week before testing and wereused only once throughout the experiments. Experiments reported in this study wereperformed in accordance with the current guidelines for the care of laboratory animals andthe ethical guidelines for investigations of experimental pain in conscious animals( Zimmerman, 1983).
2.5.
arrageenan-induced mice paw edema
 
I
nflammation of the paw in mice was induced as described previously with slightmodification (Fereira SH., 1979) 19 Mice (n=6 per group) received i.p. injection of 1 (3, 10,30 and 100 mg/kg) and aspirin (100 mg/kg, as positive control) or a similar volume of vehicle

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