Laser Physics, Vol. 6, No. 6, 1996, pp. 1211–1213.
Original Text Copyright © 1996 by Astro, Ltd. English Translation Copyright © 1996 by
/Interperiodica Publishing (Russia).
Several decades have past since the Nobel Prize win-ners Academicians A.M. Prokhorov and N.G. Basov(Russia) and Ch. Townes (USA) put forward and imple-mented the idea of quantum oscillators. At present, onecan hardly name a branch of science and technologywhere quantum oscillators are not applied (from biol-ogy and medicine to laser thermonuclear fusion). Thesuccessors made a great contribution to the develop-ment of this concept.This paper is a priority one. We pose the question asto whether it is possible to create
a laser basedon information biomacromolecules, ﬁrst of all, DNA,RNA, and chromosomes. It is difﬁcult to expect theconstruction of high-power lasers based on these struc-tures. The question is posed in a different manner: wewould like to know what new data about DNA, RNA,and chromosomes can be obtained with the help of sucha laser through the study of the parameters of its radia-tion. In our opinion, these investigations will providefundamentally new results. For example, one canobtain the data on the nonlinear dynamics of DNA,RNA, and chromosomes, including the soliton-typedynamics, rovibrational oscillations, modulation of thedispersion of optical rotation and circular dichroism,energy transfer, and some others layers of informationunavailable earlier (with the use of such methodology).Dynamic modiﬁcations of the laser beam of this typecan be of the semantic–genetic–biosymbolic type and,therefore, can possess a tremendous biological activity.Earlier, we suggested the initial ideas on this subject[1–3]. Speciﬁcally, we discussed the idea of the con-struction of a laser system using the Frölich modes [1, 2].Yu.N. Zhivlyuk (Russia) expressed an interesting ideaabout the possibility of construction of a biolasersbased on cellular structures (private communication).This idea is based on the possibility of application of phase transitions at the secondary, tertiary, and higherstructural levels of biomacromolecules.The difﬁculty of the demonstration of the correct-ness of all these ideas is associated with the fact that themajority of genetic structures containing aromatic andheterocycle rings are “transparent” within the charac-teristic spectral range of 350–400 nm. Another difﬁ-culty is that high-power pumping would inevitablydestroy “fragile” biostructures, which has been demon-strated experimentally by the research group headed byS.G. Rautiman, the Corresponding Member of the Rus-sian Academy of Sciences.To implement some of the discussed concepts, wecarried out an
investigation of the spectra of two-photon-excited luminescence (TPEL) of gel–liq-uid-crystal samples of nucleohiston, which is a sumfraction of chromosomes where histon proteins andDNA dominate (standard high-polymer preparations of Sigma). To substantially increase the intensity of TPELof genetic structures, we suggested a method of activa-tion of luminescence by the introduction of activators(donors) of TPEL into the samples under study. Theabsorption spectra of organic molecules used as activa-tors are close to those of DNA and nucleohiston. Thesemolecules are characterized by a high intensity of emis-sion spectra that lie within the areas of absorption of DNA and nucleohiston. We used crystalline dimedrolas an activator. Its structure contains a pair of benzenerings, which provides an intense asymmetric band of TPEL in a broad spectral range of 280–350 nm (thedashed line in Fig. 1).We used a copper-vapor laser for photon pumpingof the samples under study. This laser operated in astandard pulse–periodic regime at the repetition rate of
LASER METHODS IN CHEMISTRY,BIOLOGY, AND MEDICINE
Is It Possible to Create a Laser Basedon Information Biomacromolecules?
A. A. Berezin*, P. P. Garyaev*, V. S. Gorelik**, S. A. Reshetnyak**, and V. A. Shcheglov**
*Department of Theoretical Problems, Russian Academy of Sciences, Moscow, Russia**Lebedev Physical Institute, Russian Academy of Sciences, Leninskii pr. 53, Moscow, Russia
e-mail: optdep@sci.ﬁan.msk.suReceived August 2, 1996
—A method of enhancement of luminescence from information biomacromolecules by the introduc-tion of an activator (donor) into the samples under study is proposed. The experimental and theoretical studiesof two pairs of mixtures (DNA + dimedrol and nucleohiston + dimedrol) demonstrate that a superﬂuorescenceregime can be implemented in these two-component mixtures under two-photon excitation. Several intrinsicmetabolites of biosystems (nucleotides, vitamins, alkaloids, etc.) can function
as dimedrol-like activa-tors. We formulate a hypothesis that the laser pumping of genetic structures is implemented
due to theenergy of the corresponding ATP systems.
The truth always wins, but sometimes plays a draw.