Smita Tiwari et al., IJSID 2011, 1 (2), 294-302
International Journal of Science Innovations and Discoveries, Volume 1, Issue 2, November-December 2011
296The reproductive organs were removed and fixed in alcoholic Bouins Fluid. The tissues (Testes,Epididymis , Vas deferens, Seminal vesicle and Prostate) were washed in 70% alcohol and thendehydrated in alcholic series and embedded in paraffin wax, several sections were cut of 6 micron andstained with iron Hemotoxylin and Eosin for Histopathological examination. (McManus and Lowery,1965) After conducting the above experiment, histopathological studies were conducted by preparing thestained microtome slides. Histopathological studies were observed and compared with the control, underthe NaF stress.
(b) Body Weight and Organ Weights
Final body weight of each animal was recorded on the day of sacrifice . Testis, Epididymis , vasdeferens, Seminal vesicle and prostate were dissected out , and wet weights of these organs weremeasured by single pan electrical balance .
RESULTS AND DISCUSSIONTestes
Control rats consisted of highly expanded semniferous tubules with germinal epitheliumconsisting of germ cells, such as primary spermatocyte, secondary spermatocyte, spermatids andspermatozoa. The interstitial space was filled with loose connective tissue, made up of cell of Leydigs andblood vessels.
The germinal epithelial cells were found touching closely the epithelial cells of other seminiferous tubules. In the central region, spermatozoa seemed to be lesser but no significant change in the shape of seminiferous tubules were observed.
Their was significant intermingling of the seminiferous tubules. Even the spermatogonia and spermatocytes also displayeddamage and disruption.
The shape of the seminiferous tubules seemed to be quitedisintegrated and the spermatogonia and spermatocytes were damaged to a very high degree. In thecentral region of the tubule and in between the tubules the damaged cell debris has dissolved showingvacuoles.
The early stages of spermatogenesis regarding its reinitiation in hypophysectomizedrats under gonadotropic hormonal stimulation are reported by Lostroh et.al, (1963); Chowdhury andSteinberger, (1975); Chemes et al. (1976, 1979).A dose dependent reduction in body weight of NaFexposed rats is indicative of impending toxicity (Chinoy and Sequeria, 1989 b).
The transverse section of Caput epididymis presented a normal histological picture. The epithelialcells of the caput were tall, columnar with nucleus arranged in a row, near the thin basement membrane.The segments of stereocilia were more profuse in the caput region than in the cauda.
Theepithelium of the cauda consisted of low cuboidal cells. The lumen of the ductules was larger in the cauda