The full\u00adlength extra\u00adcellular form of SCF is very soluble and after preliminary
screening trials, no crystals were obtained. Screening with the 141 amino acid\u00ad
long truncated form was more successful, however crystals that were ultimately
of the orthorhombic form were quickly obtained. When these crystals were first
observed, their complexity and uniqueness were not completely appreciated. As
it turns out, the conditions for crystallization are actually quite specific and
furthermore a number of crystal types may appear within the same droplet. The
sensitivity to conditions makes it difficult to systematically evaluate the
multiplicity of crystal types, while this multiplicity complicates the subsequent
diffraction analysis. Tables 1 & 2 summarize these results.
50mM HEPES pH 7.5
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