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2009myu

2009myu

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Published by: DeLz ChangMin 심창민 on Feb 24, 2012
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PENGOLAHAN AIR ASAM TAMBANGMENGGUNAKAN BIOFILM BAKTERI PEREDUKSISULFAT
MUCHAMAD YUSRON
SEKOLAH PASCASARJANAINSTITUT PERTANIAN BOGORBOGOR2009
 
 ii
 PERNYATAAN MENGENAI DISERTASI
Dengan ini saya menyatakan bahwa disertasi Pengolahan Air AsamTambang Menggunakan Biofilm Bakteri Pereduksi Sulfat adalah karya sayasendiri dan belum diajukan dalam bentuk apapun kepada perguruan tinggimanapun. Sumber informasi yang berasal atau dikutip dari karya yang diterbitkanmaupun tidak diterbitkan dari penulis yang telah disebutkan dalam teks dandicantumkan dalam Daftar Pustaka di bagian akhir disertasi ini.Bogor, Oktober 2009Muchamad YusronNRP P062050211
 
 iii
ABSTRACT
MUCHAMAD YUSRON
. Treatment of Acid Mine Drainage using Biofilm of Sulfate Reducing Bacteria. Under supervision of DWI ANDREAS SANTOSA,BIBIANA W. LAY and ANAS MIFTAH FAUZI.Many mining and mineral processing industries discharge sulfate andmetal containing wastewater, which is called acid mine drainage. The formationof acid mine drainage is generally the result of uncontrolled oxidation of thesulfide minerals present in the terrain in which the drainage flows withconcomitant leaching of the metals. The high sulfate content in acid minedrainage can be reduced through chemical neutralization or biological treatment.Active biological treatment has been widely applied by using sulfate reducingmicroorganisms. These microorganisms can sustain life in acid environment.The aims of the research were: (i) to isolate and identify sulfate reducing bacteriacollected from acid environment,(ii) to study environmental factors that influencegrowth of sulfate reducing bacteria, (iii) to treat acid mine drainage usingsuspended cell reactor system of sulfate reducing bacteria, and (iv) to evaluate theeffectiveness of biofilm of sulfate reducing bacteria reactor for acid mine drainagetreatment.The research consists of several stages which started with explorationand identification of sulfate reducing bacteria. On the final stage, theeffectiveness of biofilm of sulfate reducing bacteria for acid mine drainagetreatment was studied. Four promising isolates of sulfate reducing bacteria whichcan sustain in acid environment were obtained from coal mining area at MuaraEnim, South Sumatra. The bacteria are classified as
 Desulfovibrio
sp., which ischaracterized as straight rods, motile, non spore-forming and able to grow insimple organic carbon. The activity of bacteria depends on environmentalconditions. The optimum pH of 
 Desulfovibrio
sp. range between 5-7, and able toreduce sulfate content of about 82-90%. Rice straw can be used as a carbonorganic source for bacterial growth. The application of freely suspended sulfatereducing bacteria cell reactor efficiently reduce sulfate and metal content of wastewaters, however operation still need long residence time. With 30 daysresidence time, 89% of sulfate content was reduced, 97% of dissolved Fe and Mnwere reduced and pH increased to 7.5. The application of biofilm of sulfatereducing bacteria cell system reactor was found more efficient in reducing sulfateand metal content of acid mine drainage. With 144 hours residence time, 77.16%of sulfate content was reduced, 88.72% of dissolved Mn and 69.72% of dissolvedFe were reduced, and pH increased up to 7. By using biofilm of sulfate reducingbacteria cell system, sulfate and metal content can be reduced to a value that meetstandard quality of industrial wastewaters within 68-80 hours residence time.Keywords : Acid mine drainage, biofilm, sulfate reducing bacteria

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