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Justify: amino acids acts as buffers

Justify: amino acids acts as buffers

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Published by Aditi Patil
BUFFERS
BUFFERS

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Published by: Aditi Patil on Nov 22, 2008
Copyright:Attribution Non-commercial

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03/21/2013

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Justify: An amino acid can act as buffer.1.In common chemical usage, a pH buffer is a substance or a mixture of substances, that permits solutions to resist large changes in the pH upon addition of small amounts of H
+
andOH
-
ions.2.A common buffer mixture contains two substances, a conjugate acid (proton donor) &conjugate base (proton acceptor). Together two species conjugate acid and conjugate baseresists large changes in pH by partially absorbing additions of H
+
or OH
-
ions to the system.3.Buffering results from two reversible reaction equilibria occurring in a solution of nearlyequal concentration of proton donor and its conjugate proton acceptor.4.All the amino acids possess a single α-amino group and a single α-carboxyl group and R-group.5.So in amino acids, α-carboxyl group acts as conjugate acid (proton donor) and α-amino groupacts as proton acceptor. Together α-carboxyl group and α-amino group resists change in pH by partially absorbing additions of H
+
or OH
-
ions to the system.6.Example : The titration curve of glycine has relatively flat zone extending about one pH uniton the either side of its midpoint, PI of 5.97. PI is the isoelectric pH of amino acid at whichamino acid is in zwitter ion form in which they are neutral or amphoteric.Figure 1: Titration curve of amino acid glycine showing shaded boxes centered about pk1= 2.34 and pk2 = 9.60 indicate the regions of greatest buffering power. pI of glycine is 5.97.

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