Jamonline /Jan-Feb 2(1) / 117–126 Madhu Babu K & Bikshal Babu K
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Journal of Atoms and Molecules
An International Online Journal An International Online Journal An International Online Journal An International Online Journal
ISSNISSNISSNISSN –––– 2277227722772277 –––– 1247124712471247
REVERSE PHASE-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THESIMULTANEOUS ESTIMATION OF AZILSARTAN MEDOXOMIL ANDCHLORTALIDONE IN PHARMACEUTICAL DOSAGE FORMSMadhu Babu Kasimala
*, Bikshal Babu Kasimala
Faculty in Chemistry, Department of Allied Sciences, College of Marine Science and Technology,Massawa, Eritrea, North East Africa.
QC Department, RV Labs, Guntur, Andhra Pradesh, India.
Received on: 01-01-2012 Revised on: 14-02-2012 Accepted on: 29–02–2012Abstract:
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated forthe simultaneous estimation of Azilsartan Medoxomil and Chlortalidone in pharmaceutical dosageforms. Isocratic elution at a flow rate of 0.9ml min
was employed on a symmetry C18 column atambient temperature. The mobile phase consisted of Methonal: Water: Acetonitrile : 0.1% Orthophosphoric acid 30:35:15:5(v/v/v/v). The UV detection wavelength was at 251 nm. The retentiontime for Chlortalidone was 3.923min and Azilsartan Medoxomil was 7.208 min. The method wasvalidated as per the ICH guidelines. The proposed method can be successfully applied for theestimation of Azilsartan Medoxomil and Chlortalidone in pharmaceutical dosage forms.
Azilsartan Medoxomil and Chlortalidone, HPLC Development, Validation, 251nm.
Chlortalidone or chlorthalidone is a diureticdrug used to treat hypertension, originallymarketed as Hygroton in the USA. It isdescribed as a thiazide diuretic (or, rather, athiazide-like diuretic because it acts similarlyto the thiazides but does not contain thebenzothiadiazine molecular structure).Compared with other medications of thethiazide class, chlorthalidone has the longest* Corresponding authorMadhu Babu Kasimala,Email: madhu.lucky09
Tel: 00291 – 7251105