Includes reports of experiments relating GAGs to cell growth control andcellular transformation; starting from 1932:Year Author 1932 Zakrezewski Reported that heparin suppresses the growthof normal embryonic tissue andJensen sarcoma tissue.1957 Sister M. Lippman Reported experimental evidence that heparincan act as a mitotic inhibitor.[This was an
study of the effect of howadministration of subcutaneous heparinaffected the measured size of Ehrlich Ascitestumors in rats. It was shown that heparin,under the conditions studied, produced a 40-50% regression of these tumors]. 1960 Ozzello
. Umbilical cord extracted hyaluronate (HA) or chondroitin sulfate(CS) promoted the growth of humanmammary carcinoma cells in culture.Any of these substance taken singly promotedtumor growth.
. Heparin prevented mitosis. Evidence for intracellular action of heparin.Heparin also caused formation of microvilion cell surfaces.(Is this an abnormal effect of heparin or is atan apparent normalintracellular function of heparin-likemolecules?)It was apparent that the phenomenon was probably not a unique property of EDTA (a high affinity Ca
chelator) which had been previouslyknown to produce microvilli formation.This outcome may simply be caused by theremoval of Ca
fromthe cell surface. (Heparin-like moleculesmay have this function