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Sterilization

Sterilization

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Published by jgcriste

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Published by: jgcriste on Dec 03, 2008
Copyright:Traditional Copyright: All rights reserved

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11/11/2012

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STERILIZATION & DISINFECTIONAntiseptics
Typically an antiseptic is a chemical agent that is applied to living tissue to kill microbes
Disinfectants
A disinfectant is a chemical or physical agent that is applied to inanimate objects to killmicrobes
Microorganism
anorganismthat ismicroscopic(usually too small to be seen by the naked human eye) Pathogenic Microorganisms
Sterility
indicates a micro-environment (the surgical field) made free of infectious microorganisms.
Disinfection
reducing the number of viable microorganisms present in a sample
Sterilization
killing of all microorganisms in a material or on the surface of an object
Bactericidal
 antimicrobialthat kills a microorganism
Bacteriostatic
antimicrobialinhibits bacterial growth but does not kill the bacteria
Methods of SterilizationA. Physical Sterilization
1.
Moist Heat
– kills all bacteria by coagulating or denaturing of the protein of the bacteria
a.Boiling (non-pressure sterilizer)b.Saturated Steam under pressure (AUTOCLAVE)
2
. Dry Heat
– recommended for use only where direct contact of material with steam isimpractical of not availablea.
Dry Heat Autoclave (hot air oven)
– used for oil, ointment, and powders.
B. Chemical Sterilization
Accomplished by use of ethylene oxide gas
Ethylene oxide is a chemical agent that kills microorganisms, including spores, byinterfering with the normal metabolism of protein and reproductive processes, resulting indeath of cells
Shelf-Life – expiration date1. Condition of Storage
Free of dust, dirt and vermin
Paper-wrapped/muslin-wrapped items good for 30 days, open shelving 21 days
Protect from extreme temperature
2. Material used for packaging
Muslin and paper wrapped items may be stored for 24-30 days, afterwhich re-sterilization is required, but if sealed in airtight plastic bag, following cooling oraerating, shelf life can be prolonged from 6-12 months
3. Seal of the package
Tape sealed packages wrapped in non-warm fabrics or plastic film can be storedfor 3-4 months
4. Integrity of the package

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