Potential of Different Light Intensities on the Productivity of
under Agra Conditions
Samuel G Singh, Mohammad Yasin Sofi and Shikha Masih
Department of Botany,
St. John’s College, Agra
-282 002, Uttar Pradesh, India
A B S T R A C T
The growth of test alga
a cyanobacterium is greatly affected by one of the mostimportant climatic factors i e light. For the successful cultivation of
with maximumbiomass, the present study has been undertaken to prescribe the optimal light intensity. So cultures werekept to different places having different intensities of light. These places were (1) Laboratory, 1800 ± 100L (2) Green house, 1600 ± 100 L (3) Outdoor 200 × 100 L. Natural light has been employed. Productivityhas been measured in terms of dried mass (g/l). The cultures which were kept under green house, highestdried mass (0.623g/l) was recorded, therefore 1600 ± 100 L of light intensity was found to be best.
, Biomass, Single cell protein
The microscopic blue green alga,
(single cell protein) is commercially producedworldwide for its proteins, vitamins and iron richcompounds. For biomass production, it is verynecessary to evaluate the optimal values of the factorsthat affect the productivity, light is one of them.Being photoautotrophic, the light is an importantfactor for the survival of the test alga,
so that it can make its own food in thepresence of optimal light. But the requirement of lightintensity for growth is different for different organisms.
also requires a specific range of intensity forits growth.Zarrouks (1966) did the first detailed study on theresponse of S
to light. In thisexperiment he reached to the conclusion that growth of alga is saturated at the level of 15-30KLux. In anotherstudy, Khan and Srivastava (2003), determined thephotoperiod for highest content of protein. But thepresent study has been undertaken to demonstrate theappropriate light intensity under the climatic conditionsof Agra to get maximum biomass of this valuable alga.
MATERIALS AND METHODS
was obtained from Centre forConservation and Utilization of Blue Green Algae,IARI, New Delhi and used as test organism.Experiments were carried out for the period of onemonth during October-November 2009. Cultivation wasdone on CFTRI medium (Venkataraman, 1983) in 2.0litres of conical flask in triplicate. One litre of eachcontains freshly prepared medium and 50ml of
inoculum. These flasks were exposed atdifferent light intensities at different places. Three setsof experiments were carried and each set containedthree flasks. First set was kept in laboratory, where thelight intensity was 1800 ± 100 L. Second set was keptin green house, where light was 1600 ± 100 L. Last setwas placed in outdoor that have highest light intensity, ie 200 × 100 L. Digital Luxmeter was used to calibratelight intensity. The productivity of the culture wascalculated in terms of dried mass (g/l). During theexperiment, agitation was done twice a day for twominutes each. Harvesting carried out by filtering thealgal culture through metal sieve of 500µm after 25days and obtained slurry was sun dried to measure theweight.
RESULTS AND DISCUSSION
i e photosyntheticbacteria, cyanobacteria and higher plants, are able toconvert light energy into chemical energy by means of photosynthesis. But the requirement of light varies fromorganism to organism. The cultures kept at differentplaces of different intensities, in this respect foroptimum growth,
has shown specific lightintensity (Table 1).Maximum growth was recorded in the culture thatwas placed in green house viz under 1600 ± 100 L of light intensity. This intensity range helped both insurvival and stimulates the growth of cultures andshowed maximum dried mass, 0.263 (g/l).Venkataraman (1983) reported the optimal growth of
on CFTRI medium following a 14 : 10 hourlight : dark cycle at the light intensity of 1600 ± 100 L.The photoperiod of the experiment carried in winterseason was different with that of Venkataraman (1983).Daytime in winter was shorter than dark time, and lightwas diffused as necessary for
cultivation.The cultures that were kept in outdoor, where thelight intensity was recorded maximum, lowest driedmass 0.267 (g/l) was observed. The intensity of 200 ×100 L did not favour the growth of
due to photoinhibition. Photoinhibition is defined as
Research Journal of Agricultural Sciences