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Table Of Contents

Natural history of HIV infection
1.1 Magnitude of problem
1.2 Biology of HIV
1.3 Pathogenesis of HIV
1.4 Factors influencing HIV disease progression
Role of CD4 T lymphocytes in disease progression
2.1 CD4 T lymphocytes and HIV
2.2 CD4 T lymphocyte counts and antiretroviral therapy
2.3 CD4 T lymphocytes counts as indicator of treatment failure
2.4 CD4 T lymphocytes counts in paediatric population
Principles of flow cytometry
3.1 Introduction
3.2 What is flow cytometry?
3.3 Instrument description
3.4 Use of fluorochromes in flow cytometry
Methods of CD4 T lymphocytes count measurement
4.1 Introduction
4.2 Flow cytometric methodologies
4.3 Selections of methodology for CD4 T lymphocytes count estimation
Alternate methods for CD4 T lymphocytes estimation
5.1 Introduction
5.2 Manual methods
5.3 Methodologies likely to become available in the near future
Quality management in CD4 T lymphocytes enumeration
6.1 Management requirements
6.2 Technical requirements
Establishing a laboratory for CD4 T lymphocytes enumeration
7.1 Suggested requirements at different levels
7.4 Establishment of a CD4 T lymphocytes enumeration laboratory
Collection and transport of specimens
General bio-safety guidelines for immunophenotyping laboratory
Pipetting techniques
Format for sample transport, receipt and report form
Useful references
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Published by Arturo Torres

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Published by: Arturo Torres on Mar 24, 2012
Copyright:Attribution Non-commercial


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