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Microbial Identification

Microbial Identification

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Published by rostaminasab
MICROBIAL IDENTIFICATION
USING THE BIOMÉRIEUX
VITEK® 2 SYSTEM
MICROBIAL IDENTIFICATION
USING THE BIOMÉRIEUX
VITEK® 2 SYSTEM

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Published by: rostaminasab on Mar 25, 2012
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03/25/2012

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MICROBIAL IDENTIFICATIONUSING THE BIOMÉRIEUX VITEK
®
2 SYSTEM
David H. Pincus
bioMérieux, Inc.Hazelwood, MO, USA
OBJECTIVE
This chapter describes the VITEK 2 automated microbiology system and itsapplication in the identification of microorganisms.
PRINCIPLES
The VITEK 2 is an automated microbiology system utilizing growth-basedtechnology. The system is available in three formats (VITEK 2 compact,VITEK 2, and VITEK 2 XL) that differ in increasing levels of capacity andautomation. Figure 1 shows the VITEK 2 compact system. All three systemsaccommodate the same colorimetric reagent cards that are incubated andinterpreted automatically.
 
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2
 
Encyclopedia of Rapid Microbiological Methods
Figure 1. VITEK 2 Compact Instrument and Workstation.
 VITEK 2 Compact
This format focuses on the industrial microbiology-testing environment whilealso having application for low to middle volume clinical laboratories. Featuresspecifically developed for industrial microbiology include 21 CFR Part 11compliance (for electronic records and signatures) and a colorimetric reagentcard (BCL) used to identify the spore-forming Gram-positive bacilli (i.e.,
Bacillus
and related genera). The other colorimetric reagent cards (GN, GP, YST) applyto all system formats for both industrial and clinical laboratories.
 VITEK 2 and VITEK 2 XL
These formats are more focused on the clinical microbiology laboratoryand provide increased levels of automation and capacity for higher volumelaboratories. They also provide an option of automatic pipetting and dilutionfor antimicrobial susceptibility testing.
Reagent Cards
The reagent cards have 64 wells that can each contain an individual testsubstrate. Substrates measure various metabolic activities such as acidification,alkalinization, enzyme hydrolysis, and growth in the presence of inhibitorysubstances. An optically clear film present on both sides of the card allows forthe appropriate level of oxygen transmission while maintaining a sealed vessel
 
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Microbial Identification Using the bioMérieux VITEK® . . .
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that prevents contact with the organism-substrate admixtures. Each card has apre-inserted transfer tube used for inoculation (described below). Cards have bar codes that contain information on product type, lot number, expirationdate, and a unique identifier that can be linked to the sample either before orafter loading the card onto the system. Figure 2 shows the GN card.
Figure 2. VITEK 2 GN Colorimetric Identification Card.
There are currently four reagent cards available for the identification ofdifferent organism classes as follows:1. GN - Gram-negative fermenting and non-fermenting bacilli2. GP - Gram-positive cocci and non-spore-forming bacilli3. YST - yeasts and yeast-like organisms4. BCL - Gram-positive spore-forming bacilliProduct-specific details for each of the identification cards are shown below.
Culture Requirements
The on-line product information contains a culture requirements table that listsparameters for appropriate culture and inoculum preparation. These parametersinclude acceptable culture media, culture age, incubation conditions, andinoculum turbidity.

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