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Chemiluminescence's Indophenols Blue Method Standard:

Industrial, Residential, Rural& other Areas Areas Ecologically Sensitive

Annual Average: 24 hr. Average

100 400

100 400

Ammonia in the atmosphere is collected by bubbling of measured amount of air through a dilute solution of sulfuric acid to form ammonium sulphate. The ammonium sulfate formed in the sample is analysed colorimetric by reaction with phenol and alkaline sodium hypochlorite to produces Indophenols a blue dye. Sodium nitropruside accelerated the reaction as an catalyst.

With a sampling rate of 1-2 lit/mina conc. range of 200-700g/m3. of air may be determine with the sampling time of one hr. The limit of detection of the analysis is 0.02NH3/ml.

Ammonia free D.D.W. Absorbing Solution (0.1 N) (2.3 ml of conc. H2SO4(18M) in 1lit.DDW.)

Sodium Nitropruside:

Sodium Hydroxide(6.75M)
(270g in 1lit.)

(2g in 100ml of DDW) (Stable for two months in refrigerator)

Buffer:

Working Hypochloride:

50g Na3PO4.12H2O in and 74ml of 6.75 NaOH in DDW. Mix 30ml of 0.1NSodium hypochloride+30ml of 6.75 M NaOH in 100ml DDW. 20ml of 45% phenol in 1ml of 2%sodium nitropruside and dilute to 100ml) (Prepare fresh every at
4hrsAmmonia:

Working Phenol:

Dissolve 3.18gm of NH4Cl in 1lit.DDW.(Stable for two month when preserve with CHCl3)

Bubble air through any gas sampling device to 10 ml of absorbing reagent. The sampling rate should be 1-2 lit/min for adequate sampling time. Transfer the sample in 25ml glass stoppred flask. Add 2ml of Buffer. Add 5ml of working phenol solution mix and then add 2.5 ml of working hypochloride solution with rapid mixing. Dilute to 25 ml and keep it in dark for 30 min. Measure developed blue colour at 630nm

Pipet 0.5,1,0,1.5 of working standard in 25ml flask and make 10 ml with absorbing and then proceed as in sample. These correspond to 5,10 and 15 g ammonia /25ml of sample.

g/m3 NH3=W/V0 Where: W=gNH3 in 25 ml from standard V= Volume of Air sampled

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