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Spore Stain (Schaeffer-Fulton Method)

Spore Stain (Schaeffer-Fulton Method)

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Published by: Abdallah Essam Al-Zireeni on Apr 10, 2012
Copyright:Attribution Non-commercial

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03/11/2013

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Spore Stain (Schaeffer-FultonMethod)& Capsule Stain
M-265 General Microbiology labYasmine Al-Shboul
 
 Although endospores were observed and reported bynumerous scientists (including Perty, 1852; Pasteur,1869; Koch, 1876; and Cohn, 1872), these structureswere primarily described as refractile bodies becausethey could not be stained using the typical compounds(carmine, methylene blue, carbol fuchsin, safranin, etc.)applied in early staining protocols.Once the endospores were understood to be a dormantform of certain bacteria that demonstrated a uniqueresistance to treatment with heat, research effortsemphasized control of the endospores to improvesterilization, prevent infection, and limit contamination offoods.
 
Spore Forming Bacteria:
 
Genus Clostridium. (Gram positive anaerobic bacteria)
Genus Bacillus. (Gram positive aerobic bacteria)
The Spore is Composed of:
 
Spore Coat.
Spore Wall.
Spore Membrane.
The Matrix (contains: DNA, RNA, Ribosoms,…)
 
The Spore is resistant to:
 
Excessive Heat, Freezing, Radiation, Desiccation, Chemical Agentsincluding common stains.

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