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Table Of Contents

Chromatography Terms are All Around Us But what do they mean…
Chromatographic Profile
Chromatographic Terms
Resolution …
Resolution—Effects of Selectivity, Efficiency, and Retention
Small Differences in αCan Provide Large Differences in Resolution
Peak Width Decreases with Increasing N – Column Efficiency
Peak Capacity
Putting it Together
Van DeemterCurve : HETP vs. Volumetric Flow Rate
Key Chromatographic Parameters Impacted by Particle Size
Calculated Effect of Particle Size on Key Chromatographic Parameters
Smaller Particle Size Columns Improve Resolution –But Pressure Increases
Mobile Phase How Does it Impact Pressure
Pressure on 2.1 x 100 mm
Some Examples of the Pressure On RRHT Columns (4.6 mm ID)
Increasing Temperature Lowers Operating Pressure
Use Elevated Temperature to Optimize Resolution, Selectivity
Conclusion
Appendix
What Extra Precautions Do You Need to Use RRHT Columns Successfully?
RRHT Column Installation Recommendations
RRHT Column Installation Recommendations (cont.)
Mobile Phase and Sample Recommendations to Avoid High Pressure
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HPLC Separation Fundamentals - 011409

HPLC Separation Fundamentals - 011409

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Published by: aishbiya on Apr 15, 2012
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07/25/2013

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