An Introduction to Immunofluorescent
Staining of Cultured Cells
Life Technologies Technical Support
Webinar Series
Presented October 5, 2011 by
Jason Kilgore, Technical Applications
Scientist Il
Life Technologies
Molecular Probes Labeling and Detection
Technologies
iThe Beauty of High Quality Fluorescence ImagesTopics to Be Covered
Inthis presentation we plan to:
= Discuss steps of an immunofluorescent staining
protocol
— material list
— common variations
- necessary controls
= Provide a simple guide for troubleshooting
- decision tree
- demonstrate common pitfalls with example images
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