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  • Antibody Ion

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    Edward Kim
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    Optimising antibody and HIER conditions Antibodies: PEG10 Mouse monoclonal (1:200-1:1000) RhoA rabbitt monoclonan (1:100-1:250) CDH1 Mouse monoclonal (1:300) HIER conditions: 92-99degC in Water Bath 20-40min blocking Step After HIER, need to mask unspecific epitopes. Primary Antibody Dilution 1 / 2 Recommend 2x Negative Recommended ed

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    Antibody ion

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    Optimising antibody and HIER conditions Antibodies: PEG10 Mouse monoclonal (1:200-1:1000) RhoA rabbitt monoclonan (1:100-1:250) CDH1 Mouse monoclonal (1:300) HIER conditions: 92-99degC in Water Bath 20-40min blocking Step After HIER, need to mask unspecific epitopes. Primary Antibody Dilution 1 / 2 Recommend 2x Negative Recommended ed

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    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    3 views3 pages

    Antibody Ion

    Uploaded by

    Edward Kim
    Optimising antibody and HIER conditions Antibodies: PEG10 Mouse monoclonal (1:200-1:1000) RhoA rabbitt monoclonan (1:100-1:250) CDH1 Mouse monoclonal (1:300) HIER conditions: 92-99degC in Water Bath 20-40min blocking Step After HIER, need to mask unspecific epitopes. Primary Antibody Dilution 1 / 2 Recommend 2x Negative Recommended ed

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    Optimising antibody and HIER conditions

    Antibodies:

    PEG10 Mouse monoclonal (1:200-1:1000) RhoA Rabbit monoclonal (1:100-1:250) CDH1 Mouse monoclonal (1:200-1:400) MYB Rabbit polyclonal (1:300)
    HIER conditions: 92-99C in Water Bath 20-40min

    HIER buffers: 10mM Citrate buffer pH6 10mM Tris EDTA buffer pH8

    Blocking Step
    After HIER, need to mask unspecific epitopes; - Normal serum corresponding to source of animal of secondary antibody HRP - Commercial blocking buffer

    Recommended antibody optimisation (Dako)

    Primary Antibody Dilution

    1/2 Recommend 2x Negative Recommended ed Recommended Control

    HIER pH6

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    HIER pH9

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    No Pretreat

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