Dialysis or diafltration?
dialyzers and Amicon
Each protein preparation is unique. Give it the special treatment it deserves witha perectly designed device or buer exchange, desalting, and removing soluteslike urea and detergents. Select between ast and gentle dialysis using the D-Tube™Dialyzers or diafltration using the aster Amicon® Ultra Centriugal Filters.
What’s the dierence?
is the traditional method or desalting (removing microsolutes) or buer/solventexchange, using
to drive solutes across a membrane.
, or ultrafltration, achieves desalting or buer exchange throughthe use o
or other external pressure to drive small microsolutesthrough a porous membrane. The membrane does not allow macrosolutes (larger thanthe pore size) to pass through.
dialyzers enable you to:
Prevent precipitation or over-concentration o intractableor sensitive samples.•
Complete dialysis in just two to fve hours, thanks to theD-Tube™ dialyzer’s double membrane, which spreads thesample over a large surace area.•
Easily handle samples in a capped centriuge tube ormatwith dialysis membrane windows.•
Achieve > 97% sample volume recovery rom solution.
Ultra centriugal flters enable you to:
•Rapidly desalt or exchange buers without diluting yoursample — just concentrate the sample, discard fltrate, andreconstitute the concentrate with desired solvent.•Repeat “washing out” until solvent exchange or desaltingis complete.•Efciently achieve salt (microsolute) transer across themembrane, regardless o microsolute concentration or sizeas quick as 30 minutes.
Ask your sample what it needs.
Based on your sample preparation needs, use the chart below to determine whether dialysis or diafltration is the best solution.
How to choose D-Tubes
dialyzers vs. Amicon
Ultra flters or dialysis and diafltration