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Dialysis or diafiltration? D-Tube™ dialyzers and Amicon® Ultra filters

Dialysis or diafiltration? D-Tube™ dialyzers and Amicon® Ultra filters

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Each protein preparation is unique. Give it the special treatment it deserves with a perfectly designed device for buffer exchange, desalting, and removing solutes like urea and detergents. Select between fast and gentle dialysis using the D-Tube™ Dialyzers or diafiltration using the faster Amicon® Ultra Centrifugal Filters.
Each protein preparation is unique. Give it the special treatment it deserves with a perfectly designed device for buffer exchange, desalting, and removing solutes like urea and detergents. Select between fast and gentle dialysis using the D-Tube™ Dialyzers or diafiltration using the faster Amicon® Ultra Centrifugal Filters.

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Categories:Types, Research, Science
Published by: EMD Millipore Bioscience on Apr 23, 2012
Copyright:Attribution Non-commercial

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05/13/2014

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Dialysis or diafltration?
D-Tube
dialyzers and Amicon
®
Ultraflters give
 YOU
the choice.
Each protein preparation is unique. Give it the special treatment it deserves witha perectly designed device or buer exchange, desalting, and removing soluteslike urea and detergents. Select between ast and gentle dialysis using the D-Tube™Dialyzers or diafltration using the aster Amicon® Ultra Centriugal Filters.
What’s the dierence?
Dialysis
is the traditional method or desalting (removing microsolutes) or buer/solventexchange, using
osmotic pressure
to drive solutes across a membrane.
Diafltration
, or ultrafltration, achieves desalting or buer exchange throughthe use o 
centriugal orce
or other external pressure to drive small microsolutesthrough a porous membrane. The membrane does not allow macrosolutes (larger thanthe pore size) to pass through.
D-Tube
dialyzers enable you to:
 
Prevent precipitation or over-concentration o intractableor sensitive samples.
 
Complete dialysis in just two to fve hours, thanks to theD-Tube™ dialyzer’s double membrane, which spreads thesample over a large surace area.
 
Easily handle samples in a capped centriuge tube ormatwith dialysis membrane windows.
 
Achieve > 97% sample volume recovery rom solution.
Amicon
®
Ultra centriugal flters enable you to:
Rapidly desalt or exchange buers without diluting yoursample — just concentrate the sample, discard fltrate, andreconstitute the concentrate with desired solvent.Repeat “washing out” until solvent exchange or desaltingis complete.Efciently achieve salt (microsolute) transer across themembrane, regardless o microsolute concentration or sizeas quick as 30 minutes.
Ask your sample what it needs.
Based on your sample preparation needs, use the chart below to determine whether dialysis or diafltration is the best solution.
How to choose D-Tubes
dialyzers vs. Amicon
®
Ultra flters or dialysis and diafltration
 
NeedD-TubeAmicon® Ultra
Faster access to sampleXSensitive samples or samples which may precipitate at higher concentrationsXRobust samples able to be concentratedXNo concentration neededXConcentration neededXLimited amounts oexchange solventXSample needs to be kept coldCold temperaturereduces speedMinimal eect o coldtemperature on speed
EMD Millipore is a division o Merck KGaA, Darmstadt, Germany

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