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How to Extract DNA from Anything Living

How to Extract DNA from Anything Living

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Published by T4urus-Vega
How to Extract DNA from Anything Living in your kitchen.
How to Extract DNA from Anything Living in your kitchen.

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Published by: T4urus-Vega on May 05, 2012
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11/07/2012

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Step 1
© 2008 University of Utah
This activity was downloaded from:
http://learn.genetics.utah.edu
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First, you need to nd something that contains DNA. Since DNA is the blueprint for life,everything living contains DNA. For this experiment, we like to use green split peas. But thereare lots of other DNA sources too, such as:SpinachChicken liverStrawberriesBroccoliCertain sources of DNA should not be used,such as:Your family pet, Fido the dogYour little sister’s big toeBugs you caught in the yardPut in a blender:1/2 cup of split peas
(
100ml)1/8 teaspoontable salt (less than 1ml)1 cupcold water (200ml)Blend on high for 15 seconds.The blender separates the pea cells fromeach other, so you now have a really thinpea-cell soup.
Blender Insanity!
Start
 
2
Soapy PeasEnzyme Power 
Pour your thin pea-cell soup through astrainer into another container (like ameasuring cup).Add 2 tablespoons liquid detergent (about30ml) and swirl to mix.Let the mixture sit for 5-10 minutes.Pour the mixture into test tubes or othersmall glass containers, each about 1/3 full.Add a pinch of enzymes to each test tubeand stir gently. Be careful! If you stir toohard, you’ll break up the DNA, making itharder to see.Use meat tenderizer for enzymes. If youcan’t nd tenderizer, try using pineapple juice or contact lens cleaning solution.
Step 3
How to Extract DNA from Anything Living
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Step 2
 
3
Alcohol Separation
What is that Stringy Stuff?
Tilt your test tube and slowly pourrubbing alcohol (70-95% isopropyl orethyl alcohol) into the tube down the sideso that it forms a layer on top of the peamixture. Pour until you have about thesame amount of alcohol in the tube aspea mixture.Alcohol is less dense than water, so itoats on top. Look for clumps of whitestringy stuff where the water and alcohollayers meet.DNA is a long, stringy molecule. The saltthat you added in step one helps it sticktogether. So what you see are clumps of tangled DNA molecules!DNA normally stays dissolved in water,but when salty DNA comes in contact withalcohol it becomes undissolved. This iscalled precipitation. The physical force of the DNA clumping together as it precipitatespulls more strands along with it as it risesinto the alcohol.You can use a wooden stick or a straw tocollect the DNA. If you want to save yourDNA, you can transfer it to a small containerlled with alcohol.
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How to Extract DNA from Anything Living
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