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Table Of Contents

Introduction
Principles
Gel media
Separation by size
Experimental parameters
Sample concepts
Column parameters
Eluent parameters
Running conditions
Characterization of solute behaviour
Theoretical considerations
Deviations from ideal behaviour in gel filtration
Application examples
Fractionation by size
Separation of monomers from dimers and higher aggregates
Determination of molecular weight distribu- tion of polymers
Determination of equilibrium constants
Desalting
Industrial applications
Properties of gel filtration media
Sephacryl HR
Superdex
Superose
Chemical and physical properties
Sephadex
Chromatographic properties
Sepharose
Sepharose CL
Experimental design
Performance
Resolution
Separation time
High performance gel filtration
Capacity
Process considerations
Choice of gel
The purpose of the experiment
Solute characteristics
Sample characteristics
Choice of running conditions
Choice of column
Flow rate
Eluent
Optimization
Performing a gel filtration experiment
Preparing the gel
Media which require swelling (Sephadex G-types)
Gel filtration in organic solvents
Packing Sephadex G types, Sepharose and Sepharose CL
Adaptors
Checking the packed bed
Using pre-packed columns
Sample application
Sample application without an adaptor
Sample application with an adaptor
Elution
Flow rates
Cleaning gels and packed columns
General cleaning procedures
Procedures to remove specific contaminants
Storage of gels and columns
Prevention of microbial growth
Antimicrobial agents
Storage of unused media
Storage of used media
Storage of packed columns
Fault finding chart
Ordering information
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Gel Filtration - Principles and Methods

Gel Filtration - Principles and Methods

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Published by Chimera Dnb

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Published by: Chimera Dnb on May 27, 2012
Copyright:Attribution Non-commercial

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