PROKARYOTES, VIRUSES, AND PROTISTANS UPDATED JUNE 2012

DID ALEXANDER THE GREAT DIE FROM WEST NILE VIRUS?

HISTORICAL REVIEW ALEXANDER THE GREAT AND WEST NILE VIRUS ENCEPHALITIS JOHN S. MARR* AND CHARLES H. CALISHER

Table. Medical history and physical examination of Alexander the Great Patient characteristics Onset of final illness May 29, 323 BC Death June 10, 323 BC Escalating fever associated with chills Excessive thirst, diaphoresis Acute abdominal pain Single episode of back pain at onset of fever Increased weakness leading to prostration with intermittent periods of energy Delirium Aphonia Terminal flaccid paralysis

MICROORGANISMS

Single-celled organisms that are too small to be seen without a microscope

Bacteria are the smallest living organisms Viruses are smaller but are not alive

THE PROKARYOTES

Only two groups

Archaebacteria and Eubacteria Arose before the eukaryotes

PROKARYOTIC CHARACTERISTICS

No membrane-bound nucleus
Single chromosome

Cell wall in most species

Prokaryotic fission

Metabolic diversity

PROKARYOTIC BODY PLAN

pilus

bacterial flagellum

DNA

plasma capsule cell wall membrane

ribosomes in cytoplasm

BACTERIAL SHAPES

coccus

bacillus

spirillum

ARCHAEBACTERIA
Methanogens
Extreme halophiles Extreme thermophiles

ARCHAEBACTERIA HABITATS

EUBACTERIA

Includes most familiar bacteria Have fatty acids in plasma membrane Most have cell wall; always includes peptidoglycan Classification based largely on metabolism

METABOLIC DIVERSITY

Photoautotrophs
Chemoautotrophs Chemoheterotrophs

BACTERIAL GENES

Bacteria have a single chromosome

Circular

molecule of DNA

Many bacteria also have plasmids

Self-replicating

circle of DNA that has a

few genes
Can

be passed from one cell to another

PROKARYOTIC FISSION

VIDEO: E. COLI REPRODUCTION

http://www.college.ucla.edu/webproject/micro 7/lecturenotes/finished/ecolivideo.mov

CONJUGATION

nicked plasmid in donor cell

conjugation tube to recipient cell

THE NORMAL FLORA

The normal flora of humans is exceedingly complex and consists of more than 200 species of bacteria. The mixture of organisms regularly found at any anatomical site is referred to as the normal flora.

A FRIENDLY BACTERIA: E. COLI

Commonly inhabits the intestines of vertebrates E. coli does not normally infect us Many benefits: vitamin K and the B complex are produced by E. coli prevent colonization by pathogens stimulate the production of cross-reactive antibodies

E. COLI STRAIN O157:H7. A DANGEROUS FORM

This mutant can be transmitted in food or water Think White Water and the Jack in the Box hamburger incidents Both made national news when many people became ill and some died.

SEWAGE POLLUTION INDICATOR

E. coli in streams, drinking water and surface waters of all kinds may indicate a problem with sewage and or farm runoff. Testing for unusual levels of these organisms is routinely done by public health officials Parks, swimming pools, restaurants etc may be closed if E. coli is detected

E. COLI PHOTOS

EM cells
http://www.bact.wisc.edu/bact330/nfE.coli_colonies.jpeg

Culture plate

PARK SETTLES LAST E. COLI SUIT

Patricia Guthrie - Staff Friday, December 15, 2000 The last of a dozen lawsuits, filed by families of children sickened in a 1998 E. coli outbreak tied to Cobb County's White Water park, has been settled out of court for an undisclosed sum, attorneys said Thursday. The family of Jordan Faith Shook of Cartersville, whose symptoms were among the most severe of the 26 children sickened by E. coli O157:H7, agreed to an out-of-court settlement. The settlement was sealed in court records and will not be disclosed, said Bill Marler, attorney for James and Judy Shook, Jordan's parents

http://www.marlerclark.com/news/whitewater10.htm

NECROTIZING FASCIITIS: "THE FLESHEATING BACTERIA"

Streptococcus pyogenes strains (as well as others) rarely cause necrotizing fasciitis These infections are extremely serious Sometimes necessitate amputation or result in severe disfigurement.

PHOTOMICROGRAPH OF STREPTOCOCCUS PYOGENES

http://www.cellsalive.net/photos/images/strep1.jpg

UNEXPECTED CONSEQUENCES

One weekend in June of 1998 while Cassie Moore was camping with her three children, she obtained a minor cut on her finger, which she bandaged properly. She also injured the left side of her body participating in sports. Not thinking much of either, she bandaged the cut, and went to bed (Moore, 1999).

edu:81/ScienceEd/stories/storyReader$89

WARNING!

The next two slides are very graphic. They show the results of necrotizing fasciitis!! You dont have to look at them if you are squeamish!

SEVERE CASE

http://www.bact.wisc.edu:81/ScienceEd/stories/storyReader$89

AMPUTEE

MICROBES IN THE NEWS

RECENTLY IN GEORGIAMORE FLESH EATING BACTERIA http://video.search.yahoo.com/search/video; _ylt=A0oG7mzFUdNPm3UAQDdXNyoA?p=g eorgia+women+flesh+eating&fr=mymyy&fr2=piv-web You can also check this out: http://www.npr.org/blogs/health/2012/01/13/1 45179057/woman-injects-bath-salts-losesarm-to-flesh-eating-bacteria

(5/16/2012) (5/24/2012)

AEROMONAS INFECTIONS
Aeromonas is a common aquatic potential deadly infectious bacteria. Victims usually are infected in deep lacerations that are exposed to fresh water, either standing or running. Warning: This video has a series of graphic images which some my find disturbing. http://video.search.yahoo.com/video/play?fr= my-myy&ei=UTF8&c=2&p=aeromonas+infections&vid=44d6d

EPA FINDS CONTAMINATED DRINKING WATER ON PLANES

WASHINGTON (Reuters) -- A surprising number of drinking water systems on domestic and foreign commercial aircraft tested this summer by the government did not meet federal standards because they were contaminated with potentially harmful bacteria, regulators said Monday. (Tuesday, September 21, 2004 Posted: 10:46 AM EDT (1446 GMT) )

E. COLI OUTBREAK ALARMS GERMANY AS YOUNG WOMEN SICKEN (BBC 25 MAY 2011)

Germany is alarmed at the scale of an E. coli food poisoning outbreak which is thought to have killed three people and may have infected hundreds more.

Salads suspected health conscious women.

E. COLI CUCUMBER SCARE: SPAIN ANGRY AT GERMAN CLAIMS BBC 31 MAY 2011

Spain has expressed anger at links being made between Spanish cucumbers and a deadly E. coli outbreak. http://www.bbc.co.uk/news/world-europe13605910

Reaction to the cucumber crisis Country Action Consumers told not to eat cucumbers, lettuces and raw tomatoes. 1,150 cases of E.coli confirmed; 14 deaths One death and 36 suspected E. coli infections, linked to travel in northern Germany. Top European cucumber producer - threatens to seek compensation from the European Union for lost vegetables sales Ban on all imports of cucumbers, tomatoes and fresh salad from Spain and Germany pending further notice Some Spanish-grown cucumbers removed from sale Some Spanish-grown cucumbers removed from sale Ban on sale of cucumbers, tomatoes and aubergines imported via Germany Reported to have banned cucumber imports from Spain Halted all cucumber shipments to Germany Testing cucumbers for

Germany

Sweden

Spain

Russia

Czech Republic

France

Austria

Belgium Netherlands Denmark

THE SOURCE: SUSPECTED EGYPTIAN SPROUT SEEDS STILL ON SALE

Egyptian sprout seeds suspected to be contaminated with the deadly E.coli bacteria which has caused a rampaging outbreak in Europe are still believed to be on sale. The EU has belatedly announced a blanket ban on more imports. 4000 ill; 51 dead July 6, 2011 Posted in: Health News

http://www.ygoy.com/2011/07/06/suspected-egyptian-sproutseeds-still-on-sale/

BACTERIA-RICH HAILSTONES ADD TO 'BIOPRECIPITATION' IDEA (BBC 25 MAY 2011)

A study of hailstones has found large numbers of bacteria at their cores.

The find lends credence to the "bio-precipitation" idea, which suggests that bacteria are actively involved in stimulating precipitation. The bacteria have protein coatings that cause water to freeze at relatively warm temperatures.

PREVENTING SEPTIS

http://videos.howstuffworks.com/universityof-chicago/2127-smart-bacteria-in-your-gutvideo.htm

BACTERIA 'LINKED' TO PARKINSON'S DISEASE (BBC 22 MAY 2011)

The bacteria responsible for stomach ulcers have been linked to Parkinson's disease, according to researchers in the US.
Mice infected with Helicobacter pylori went onto develop Parkinson's like symptoms.Parkinson's disease affects the brain and results in slow
movements and a tremor.

The researchers believe the bacteria are producing chemicals which are toxic to the brain.

Dr. Testerman said this new chemical was almost identical

to one found in seeds from the cycad plant, which had been shown to trigger a Parkinson's-like disease among people in Guam.

OVERUSE OF ANTIBIOTICS INCREASES THE RISK OF OBESITY.

In Theory: In Some Cases, Even Bad Bacteria May Be Good By KATE MURPHY Published: October 31, 2011 THE HYPOTHESIS Overuse of antibiotics increases the risk of obesity. THE INVESTIGATOR Dr. Martin Blaser, New York University Langone Medical Center.
http://www.nytimes.com/2011/11/01/health/scientistexamines-possible-link-between-antibiotics-and-obesity.html

SURPRISE: ANTIBIOTICS MAY BE CONTRIBUTING TO THE OBESITY EPIDEMIC

Microbiologists at New York University have published a new study that says the overprescribing of antibiotics could be making us fat! Researchers fed infant mice low doses of penicillin; after 30 weeks, penicillin-fed mice were between 10 and 15 per cent bigger and twice as fat as drug-free mice. This affirms research from Copenhagen which found that infants given antibiotics within the first six months of life were more likely to be overweight at age 7, even if their mother was of a healthy weight.
http://www.anh-usa.org/antibiotics-may-be-contributing-to-the-obesity-epidemic/

Foodborne Illness

SALMONELLA

FROM CBS

http://www.youtube.com/watch?v=s7_QPeFY Tx8&feature=fvsr

CHARACTERISTICS OF SALMONELLA
Gram negative Rod shaped (bacillus) Non-spore forming Motile (many flagella) Enterobacteria (Live in the intestines of many animals) Chemoorganotrophs Salmonella infections are zoonotic

http://en.wikipedia.org/wiki/Salmonella

GRAM STAINING

Laboratory staining protocol developed to help identify bacteria

Two stains are used on heat-fixed (death by heat) smear of a bacterial culture
Stain #1 is crystal violet which stains the bacterial cells purple Stain #2 is usually safrarin which stains the bacterial cells red or pink Gram + bacteria appear purple under microscope because they retain the crystal violet dye in their cell walls Gram- bacteria appear red or pink under the microscope because they do not retain the blue dye, but do retain the pink dye Does not work on all types of bacteria

GRAM STAINING VIDEO

BEST Gram Staining Video Ever!!! http://www.youtube.com/watch?v=CyyY8h1d oJk

Second Best http://www.youtube.com/watch?v=OQ6Cgj_UHM&feature=grec_index

UNDER THE MICROSCOPE

A Gram stain of mixed Staphylococcus aureus (Gram positive cocci) and Escherichia coli (Gram negative bacilli)

http://en.wikipedia.org/wiki/Gram_staining

SALMONELLA AND DISEASE

The Salmonella family includes over 2,300 serotypes of bacteria which are one-celled organisms too small to be seen without a microscope. Two types, Salmonella enteritidis and Salmonella typhimurium are the most common in the United States and account for half of all human infections. Strains that cause no symptoms in animals can make people sick, and vice versa. If present in food, it does not usually affect the taste, smell, or appearance of the food. The bacteria live in the intestinal tracts of infected animals and humans.
http://www.fsis.usda.gov/factsheets/salmonella_questions_&_answers/index.asp

SALMONELLOSIS

Salmonellosis is an infection with bacteria called Salmonella. Most persons infected with Salmonella develop diarrhea, fever, and abdominal cramps 12 to 72 hours after infection. The illness usually lasts 4 to 7 days, and most persons recover without treatment. However, in some persons, the diarrhea may be so severe that the patient needs to be hospitalized. In these patients, the Salmonella infection may spread from the intestines to the blood stream, and then to other body sites and can cause death unless the person is treated promptly with antibiotics. The elderly, infants, and those with impaired immune systems are more likely to have a severe illness.
http://www.cdc.gov/nczved/divisions/dfbmd/diseases/salmonellosis/

DEATHS
About 142,000 (reported) Americans are infected each year with Salmonella enteritidis from chicken eggs, and about 30 die.[18] The shell of the egg may be contaminated with salmonella by feces or environment (common), or its interior (yolk) may be contaminated by penetration of the bacteria through the porous shell or from a hen whose infected ovaries contaminate the egg during egg formation (unlikely). http://en.wikipedia.org/wiki/Salmonella

FDA TIES CHICKEN FEED TO SALMONELLA IN EGG RECALL

WASHINGTON (Reuters) Bacteria found in chicken feed used at two Iowa farms has been linked to a salmonella outbreak that prompted the recall of more than a half billion contaminated eggs, U.S. regulators said on Thursday.
By Alina Selyukh Alina Selyukh Thu Aug 26, 5:24 pm ET

SO WHAT'S HAPPENING TO THE RECALLED EGGS AND THE FRESH Eggs from that massive salmonella outbreak could ONES?
still end up on a store shelf near you.
CBS News has learned that some of the recalled eggs are being sent to egg processing facilities, along with fresh ones that infected hens are still producing. The eggs will be cooked, pasteurized and used in products like ice cream and mayonnaise. The FDA says it's legal and safety experts insist there's little risk to consumers. http://ozarksfirst.com/fulltext?nxd_id=316835

THE GOOD GUYS.

Food Production: Probiotics Probiotics are living organisms that, when consumed, have beneficial health benefits outside their inherent nutritional effects. There is a growing body of evidence for the role of probiotics in gastrointestinal infections, irritable bowel syndrome and inflammatory bowel disease.[3][4] Lactobacillus species are used for the production of yogurt, cheese, sauerkraut, pickles, beer, wine, cider, kimchi, chocolate and other fermented foods, as well as animal feeds such as silage. In recent years, much interest has been shown in the use of lactobacilli as probiotic organisms and their potential for disease prevention in humans and
[3][6]

Bifidobacteria are considered as important probiotics, and are used in the food industry to relieve and treat many intestinal disorders. Bifidobacteria exert a range of beneficial health effects, including the regulation of intestinal microbial homeostasis, the inhibition of pathogens and harmful bacteria that colonize and/or infect the gut mucosa, the modulation of local and systemic immune responses, the repression of procarcinogenic enzymatic activities within the microbiota, the production of vitamins, and the bioconversion of a number of dietary compounds into bioactive molecules.[4][6] http://en.wikipedia.org/wiki/Food_microbiology#Probiotics

VIRUS
Noncellular infectious agent Consists of protein wrapped around a nucleic acid core Cannot reproduce itself; can only be reproduced using a host cell

HPV CARRIED IN MOUTH LINKED TO VIRULENT CANCERS NUMBER OF OROPHARYNGEAL CASES STEADILY RISING, STUDY FINDS

The Washington Times Thursday, January 26, 2012 The new study finds that the most prevalent HPV strain found in peoples mouths is HPV-16, a type that is particularly likely to cause cancers, Dr. Maura L. Gillison and her colleagues said. An estimated 2.13 million Americans have an oral HPV-16 infection, the authors said in their study, published in the Jan. 26 issue of JAMAs Online First edition. Men are almost three times as likely as women to have oral HPV, with 10.1 percent of men and 3.6 percent of women testing positive for it.

VIRAL BODY PLANS

Genetic material is DNA or RNA Coat is protein

Helical virus

Polyhedral virus

Complex virus (bacteriophage)

ENVELOPED VIRUS (HIV)

lipid envelope; proteins span the envelope, line its inner surface, spike out above it

viral coat (proteins)

VIRAL MULTIPLICATION - BASIC STEPS

Virus attaches to host cell Whole virus or genetic material enters host Viral DNA or RNA directs host to make viral genetic material and protein Viral nucleic acids and proteins are assembled New viral particles are released from cell

LYTIC PATHWAY

Lysis

Assembly

Assembly Virus injects genetic material

Production of viral components

LYSOGENIC PATHWAY
Latent period extends the cycle Viral DNA becomes part of host chromosome for a time

Stimulus may cause cell to enter lytic pathway

Viral material integrated

Viral material passed on

VIDEO: VIRUS REPRODUCTION

http://www.med.sc.edu:85/movies/phageh.m ov

REPLICATION OF AN ENVELOPED VIRUS

Transcription of viral genes DNA replication

Translation Proteins

Assembly

FOOD AND WATER BORNE VIRUSES

Norwalk and Norwalk-like viruses Polio hepatitis A (HA) Massive virus discovered in water tower

NORWALK AND NORWALK-LIKE VIRUSES

small round structured viruses (SRSVs) strand RNA single structural protein viral gastroenteritis self-limiting, mild, and characterized by nausea, vomiting, diarrhea, and abdominal pain.

http://rhino.bocklabs.wisc.edu/virusworld/jysart/norwalk_asv2001.jpg

DISNEY SHIP DOCKS WITH 195 ILL

PORT CANAVERAL, Florida (CNN) -- Sunny skies greeted the Disney cruise ship Magic as it docked here early Saturday, carrying 195 sick passengers and crew members. Norwalk virus.

(2002)

http://www.cnn.com/2002/TRAVEL/11/30/disney.sick.cruise/

MASSIVE VIRUS DISCOVERED IN WATER TOWER

The largest virus ever discovered has been found in a water-cooling tower in Bradford, England. It was lurking inside single-celled organisms called amoebae, but its discoverers believe that it may also be capable of infecting humans.

http://www.newscientist.com/news/news.jsp?id=ns99993559

PHOTO FROM THE NEW SCIENTIST

girth of 400 nanometers (visible with a good light microscope) 900 genes

POLIO
Small water-food borne RNA virus In about 1% of the infected population, the virus attacks and kills motor neurons This results in various degrees of paralysis

http://www.rednova.com/news/stories/2/2003/04/29/story004.html

POLIOS EFFECTS

http://cha.state.md.us/edcp/html/polio.html

http://www.bret.org.uk/nec2.htm

HEPATITIS A (HA) SYMPTOMS

fatigue jaundice abdominal pain loss of appetite nausea diarrhea fever

PERSONS AT RISK

Household contacts of infected persons Sex contacts of infected persons Persons, especially children, living in areas with increased rates of hepatitis A during the baseline period from 1987-1997. Persons traveling to countries where hepatitis A is common Men who have sex with men Injecting and non-injecting drug users

AVERAGE REPORTED CASES OF HEPATITIS A PER 100,000 POPULATION*, 1987-1997

Red> 20

THE HA WORLD

TRANSMISSION
HAV is found in the stool (feces) of persons with hepatitis A. HAV is usually spread from person to person by putting something in the mouth (even though it may look clean) that has been contaminated with the stool of a person with hepatitis A.

PREVENTION

Hepatitis A vaccine is the best protection. Short-term protection against hepatitis A is available from immune globulin. It can be given before and within 2 weeks after coming in contact with HAV. Always wash your hands with soap and water after using the bathroom, changing a diaper, and before preparing and eating food.

VACCINATION

Travelers to areas with increased rates of hepatitis A Men who have sex with men Injecting and non-injecting drug users Persons with clotting-factor disorders (e.g. hemophilia) Persons with chronic liver disease Children living in areas with increased rates of hepatitis A during the baseline period from 19871997.

http://www.cdc.gov/ncidod/diseases/hepatitis/a/fact.htm

HA JAUNDICE AND THE VIRAL PARTICLE

http://www.apotheke-im-globus-wachau.de/Service/reis http://www.webcolombia.com/health/hepatitis/sintomas.jpg

OUTBACK CHAIN ACQUIRES CHI-CHI'S

LOUISVILLE, Ky. (AP) Chi-Chi's, the chain of Mexican restaurants, may have served its last chimichanga. Outback Steakhouse Inc. this week closed on its $42.5 million deal for the rights to 76 restaurants in the Chi-Chi's chain, which was beleaguered by bankruptcy and a hepatitis outbreak.

http://www.washtimes.com/business/20040922-091157-9257r.htm

DRY AIR CAUSES WINTER FLU OUTBREAKS

DISCOVERY NEWS WED MAR 3, 2010 03:44 AM ET

Researchers link flu outbreaks with low humidity levels of winter. Winters in New York are four times less humid than in summer. In Minnesota, humidity can drop five-fold. Linking the flu to absolute humidity could help health workers prepare for outbreaks.

VIROIDS

Smaller than viruses

Strands or circles of RNA

No protein-coding genes
No protein coat

Cause many plant diseases

PSTV
Most known viroids cause diseases in plants. The first viroid was discovered in 1971, by Diener. It's called the potato spindle tuber virus (PSTV), Contains a single loop of RNA Relies wholly on enzymes all ready in the host cell

http://math.ucr.edu/home/baez/subcellular.html#Viroids

VEGETABLE MD ONLINE

Cornell University Ag School

http://vegetablemdonline.ppath.cornell.edu/Images/Potatoes/PotatoViruses/PotatoVirusfs1.jpg

PRIONS
Small proteins Linked to human diseases

Kuru Creutzfeldt-Jakob

disease (CJD)

Animal diseases
Scrapie

in sheep Bovine spongiform encephalopathy (mad cow disease)

BSE (MAD COW DISEASE)

There is a disease similar to BSE called Creutzfeldt-Jacob Disease (CJD) that is found in people.

WARNINGS SENT TO EMORY BRAIN PATIENTS

ATLANTA Oct. 1, 2004 Emory University officials sent warning letters to more than 500 surgery patients at the school's medical center after a brain surgery patient tested positive for a fatal disease similar to the human version of mad cow disease. Chances of infection are very low, said Dr. William Bornstein, chief quality officer for Emory Healthcare. "By using modern sterilization, this has never been transmitted," he said.

http://abcnews.go.com/wire/Living/ap20041001_1791.html

VIDEO: UK MAD COW DISEASE

From the Asian Discovery Channel http://www.youtube.com/watch?v=bQk5UnW gqUE

IN THE NEWS

http://www.youtube.com/watch?v=RUnoaSK nHXY&feature=related

MOLECULAR MECHANISM

http://www.youtube.com/watch?v=H2Ouxl_G NjA&feature=related

PROTISTANS DIFFER FROM PROKARYOTES

Have a nucleus and organelles Have proteins associated with DNA Use microtubules in a cytoskeleton, spindle apparatus, and cilia and flagella May contain chloroplasts May divide by mitosis and meiosis

MAJOR LINEAGES
Heterotrophs
Chytrids Water

Autotrophs

Mix or Both

molds Slime molds Protozoans Sporozoans

Red algae Euglenoids Brown algae Dinoflagellat e Green algae Golden algae Diatoms

ANIMAL-LIKE PROTISTANS
Informally known as protozoans May resemble single-celled heterotrophic protistans that gave rise to animals Include predators, parasites, and grazers

MAJOR GROUPS OF PROTOZOANS

Sarcodina - Amoeboid protozoans Ciliphora - Ciliated protozoans Mastigophora - Animal-like flagellates Apicomplexa - Parasitic heterotrophs such as the sporozoans

NAKED AMOEBAS

Change shape constantly Move by means of pseudopods Most are free-living cells that engulf their prey Some are symbionts in animal guts A few are opportunistic pathogens

AMOEBA

AMOEBA

VIDEO: AMOEBA

http://micro.magnet.fsu.edu/moviegallery/pon dscum/protozoa/amoeba/

FLORIDA BOY DIES FROM AMOEBA INFECTION IN BRAIN

(CNN) -- An unidentified 12-year-old died Friday after being infected with an amoeba while swimming in a Florida lake. The boy had meningeal encephalitis -- a combination of meningitis and encephalitis, which causes the brain to swell, according to Dr. Jaime Carrizosa, an infectious disease specialist from Florida Hospital in Orlando. Carrizosa had treated the boy.

http://www.rense.com/general27/oom.htm

OTHER AMEBOID PROTOZOANS

Foraminiferans
Calcium

carbonate shell

Radiolarians and Heliozoans

Shells

of silica

FORAMINIFERAN

Shelled amoeba, calcium carbonate

Do not post photos on Internet

Shelled amoeba, silica

A living heliozoan

CILIATED PROTOZOANS

Phylum Ciliphora All heterotrophs Arrays of cilia allow movement and direct food into oral cavity

Hypotrich

BODY PLAN OF PARAMECIUM

food residues being ejected food vacuole

gullet

cilia trichocysts (harpoons)

contractile vacuole emptied macronucleus micronucleus

contractile vacuole full

CILIATE CONJUGATION

Most ciliates have two different nuclei

Large

macronucleus
micronucleus

Smaller

Micronucleus participates in sexual reproduction (conjugation)

Partners

exchange micronuclei

PARAMECIUM CONJUGATION

VIDEO: CILIATES

http://micro.magnet.fsu.edu/moviegallery/pon dscum/protozoa/bursaria/index.html

VIDEO: DIDINIUM

http://video.search.yahoo.com/video/view?&h =78&w=115&type=msmedia&back=p%3Ddid inium%26ei%3DUTF8%26fl%3D0%26fr%3DFP-tab-webt&vurl=www.wynantskillufsd.org%2FMiddle% 2520Grades%2FMesh%2FScience%2FLife %2520Science%2FLife%2520Processes%2 FEnergy%2FVideo_Clips%2

ANIMAL-LIKE FLAGELLATES

Phylum Mastigophora
Move by means of flagella All are heterotrophs
Free-living

species in freshwater and marine habitats

Many

are internal parasites

BODY PLAN OF A TRYPANOSOME (CAUSES AFRICAN SLEEPING SICKNESS)

Undulating membrane

mitochondrion

basal body of flagellum

free flagellum nucleus

TRICHOMONAS VAGINALIS (COMMON STD)

TRICHOMONAS VAGINALIS

Trichomonas vaginalis is a sexually transmitted disease (STD), although transmission by other routes (such as soiled towels) has been documented.

Most people infected with trichomoniasis are asymptomatic. Symptomatic infections are characterized by a white discharge from the genital tract and itching.

http://www.biosci.ohio-state.edu/~parasite/trichomonas.html

DINOFLAGELLATES

Red tide organisms

RED TIDE

VIDEOS: RED TIDE

From the Mote Marine Laboratory, FL http://www.youtube.com/watch?v=0LdLWPwdw Vs San Diego, CA http://www.youtube.com/watch?feature=endscre en&v=eFY7rbEOH-8&NR=1

Amazing blue glowing waves at La Jolla Shores beach caused by the red tide that hit San Diego on September 29, 2011. The bright blue color is caused by bioluminescence from an algae bloom. The algae is red in the day, hence the name Red Tide, but glows a bright blue at night when agitated, such as when a wave breaks. It's a really awesome sight to see.... when people swim in the water, the entire water around them glows blue. Truly amazing!

http://www.youtube.com/watch?feature=endscre en&v=Py-J1ZazHDM&NR=1

RED TIDE TOXINS CAN CAUSE RESPIRATORY PROBLEMS IN HUMANS

Source: Boston Globe, 3/29/05

SPOROZOANS

Parasitic
Complete part of the life cycle inside specific cells of a host organism Many have elaborate life cycles that require different hosts

Many cause serious human disease

CRYPTOSPORIDIUM

Motile infective stage (sporozoite)

invades intestinal epithelium

Causes cramps, watery diarrhea

Commonly transmitted by water

contaminated with cysts

TOXOPLASMA
Cysts may be ingested with raw or undercooked meat Exposure to cysts from cat feces Symptoms are usually mild in people with normal immune function Infection during pregnancy can kill or damage the embryo

CAT VECTOR (TOXOPLASMA)

CAT PARASITE 'IS KILLING OTTERS'

BBC News website science reporter, St Louis 19 Feb 2006

Cat faeces carrying Toxoplasma parasites wash into US waterways and then into the sea where they can infect otters, causing brain disease.

MALARIA

Symptoms have been known for more than 2,000 years

Most prevalent in tropical and subtropical parts of Africa Kills a million Africans each year Caused by four species of Plasmodium Transmitted by Anopheles mosquitoes

PLASMODIUM LIFE CYCLE

sporozites

sporozites Gametes form in mosquito gut, combine to form zygotes

merozoite Offspring enter blood, cause malarial symptoms

Male and female gametocytes in blood

MALARIA DEATHS HUGELY UNDERESTIMATED - LANCET STUDY BBC 2 FEBRUARY 2012

The research, published in the British medical journal the Lancet, suggests 1.24 million people died from the mosquito-borne disease in 2010. This compares to a World Health Organization (WHO) estimate for 2010 of 655,000 deaths. But both the new study and the WHO indicate global death rates are now falling. The rise in malaria deaths up to 2004 is attributed to a growth in populations at risk of malaria, while the decline since 2004 is attributed to "a rapid scaling up of malaria control in Africa", supported by international donors.

A SPOROZOAN CAUSES BAGDAD BOILS

Eck Family Institute http://www.youtube.com/watch?v=T-mD7jzUklQ Cutaneous leishmaniasis (also known as "Aleppo boil," "Baghdad boil,"
"Bay sore," "Biskra button," "Chiclero ulcer," "Delhi boil," "Kandahar sore," "Lahore sore,"

is the most common form of leishmaniasis. It is a skin infection caused by a single-celled parasite that is transmitted by sandfly bites. There are about 20 species of Leishmania that may cause cutaneous leishmaniasis. Leishmania currently affects 12 million people in 88 countries. Wkipedia
"Leishmaniasis tropica," "Oriental sore," "Pian bois," and "Uta"[1]:423)

EUGLENOIDS
Phylum Euglenophyta Free-living flagellated cells that live in freshwater Majority are photoautotrophs Some are heterotrophs that feed on dissolved organic compounds Sewage pollution indicator organisms

EUGLENOID BODY PLAN

long flagellum contractile vacuole chloroplast

eyespot shielding a ER light-sensitive receptor nucleus Golgi body

mitochondrion pellicle

VIDEO: EUGLENA

http://www.youtube.com/watch?v=J3sgoy22 Ij4

CHRYSOPHYTES

Phylum Chrysophyta
Mainly free-living photosynthetic cells

Four groups:
- Golden algae - Yellow-green algae - Diatoms - Coccolithophores

MIXED DIATOMS

DIATOM CHARACTERISTICS
Cell wall is composed of silica Two valves (halves) similar to a Petri dish Golden/green in color Important primary producers in both marine and freshwater ecosystems

VIDEO: DIATOMS

http://www.youtube.com/watch?v=gVIP0AWDd4&feature=results_main&playnext= 1&list=PL4333AE134CD94B3E

CENTRIC AND PENNATE DIATOMS

GREEN ALGAE

Phylum Chlorophyta 7,000 species Resemble plants

Chlorophylls a and b Starch grains in chloroplasts Cell walls of cellulose, pectins
Ulva

CHLAMYDOMONAS LIFE CYCLE

Zygote Diploid Haploid

Nuclear fusion

Meiosis, germination

Mitosis Cytoplasmic fusion Asexual reproduction Asexual reproduction

Sexual reproduction Gametes meet

CONJUGATING SPIROGYRA

GREEN TECHNOLOGY

From the History Channel http://www.history.com/shows/modernmarvels/videos/greenalgae?cmpid=MRSS_Bing_HIS#green-algae