G-Biosciences, St Louis, MO. USA
A Geno Technology, Inc.
(USA) brand name
think proteins! think G-Biosciences! www.GBiosciences.com
1-Step RT-PCR Kit
The 1-Step RT-PCR Kit is designed for optimal convenience in carrying out highly sensitive and specific RT-PCR in a singletube. 1-Step RT-PCR is a variation on standard (two-step) RT-PCR in which all components of the RT and PCR are mixed inone tube prior to starting the reactions and in which RT and PCR are thus carried out sequentially in one tube. This approachoffers tremendous convenience when applied to analysis of single targets from multiple samples of RNA. This approach alsominimizes the possibility for introduction of contaminants into reactions between the RT and PCR steps, since the RT andPCR reactions are carried out sequentially, without opening of reaction tubes between steps.The kit is composed of high quality reagents. The AMV reverse transcriptase, from Avian Myeloblastosis Virus, is used forRT, and the optimized Taq DNA polymerase is used for PCR. A unique buffer, 10×RT-PCR buffer suitable for both the RTand PCR steps and crucial for successful 1-Step RT-PCR, is included, along with dNTPs mixture and Ribonuclease Inhibitor.DEPC-treated water is also provided. Use of this kit and the accompanying protocol will ensure excellent results.
ITEMS SUPPLIED Cat. # 786-813Part. # Description Size
219A-B AMV Reverse Transcriptase [25U/µl] 125U003T-I Taq DNA Polymerase [5U/µl] 125U068R-A RT-PCR Buffer [10X] 50µl210D-A dNTPs [10mM each] 25µl079R-B Ribonuclease Inhibitor [40U/µl] 300U025D-F DEPC-treated water 0.5ml
The kit is shipped on blue ice. Upon arrival, store
at -20°C. This product is stable for 1 year at -20
C.The AMV Reverse Transcriptase is supplied in 200mM potassium phosphate (pH7.2), 2mM DTT, 0.2% Triton X-100, 50%Glycerol.
AMV Reverse Transcriptase: Avian Myeloblastosis Virus
Taq DNA Polymerase: The polymerase gene from Thermus aquaticus (strain YT1) is cloned and expressed in
,then highly purified to produce Taq DNA polymerase.
AMV Reverse Transcriptase: One unit is defined as the amount of enzymes that incorporate 1nmol of dTTP into acid-insoluble precipitate in10 min using poly(A)
RNA and oligo(dT) as the template/primer at 37°C.Taq DNA Polymerase: One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze theincorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herringsperm DNA as a substrate.