Assignment on Regulation of Transcription, Lytic Lysogeny cascade and SOS regulatory system
When isolated from bacteria, prokaryotic RNA polymerase has twoforms: The
. The core enzyme is atetramer whose composition is given as
(two alpha subunits, one beta
subunit, and one beta-prime subunit). Core RNA polymerase is capable of faithfully copying DNA into RNA but does not initiate at the correct site in agene. That is, it does not recognize the promoter specifically. Correct promoterrecognition is the function of the holoenzyme form of RNApolymerase.
The RNA polymerase holoenzyme contains another subunit, s(
), inaddition to the subunits found in the core enzyme. Holoenzyme,
capable of correct initiation at the promoter region of a gene. Sigma thus mustbe involved in promoter recognition. Sigma subunits are related but distinct indifferent forms of RNA polymerase holoenzyme. These specialized subunits
direct RNA polymerase to promoter sequences for different classes of genes.For example, bacteria exposed to high temperatures synthesize a set of protective proteins called
. The genes for the heat-shockproteins have special promoter sequences that are recognized by an RNApolymerase holoenzyme with a specific subunit. The discussed here is the
major of the common bacterium
, about which most is known.
RNA polymerase holoenzyme starts by recognizing the promoter of a gene. The promoter isn't copied into RNA, but it is, nonetheless, an important piece
Submitted by: Shraddha Bhatt.Page