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San Diego Watershed Monitoring ProgramInstructions for Proper Use of Sampling Equipment 
I.Bag Labeling Instructions
Using a Sharpie marker, please label each sampling bag with the followinginformation. The first 4 things can be labeled prior to departure to field.
Site ID
Watershed Name
Date
Sampler’s name
Time
(fill out in field just beforesample is collected!!)
(If the bag is wet, wipe the bag with paper towel first, then label with sharpie.)
ONCE THE BAGS ARE LABELED YOU CAN BEGIN COLLECTING YOURWATER SAMPLES.
II. Sample Collection and Storage
Fill 
two (2)
100 ml (small)Whirl-Pak sampling bags
Fill
four
(
4
) 500 ml (large)Whirl-Pak sampling bags
1
 
WARNING: Only collect a sample when the water source is flowing. If thewater source is stagnant, do not collect a sample unless the water source flows on top of an unpaved surface or natural bottom. Be sure to note on thedata sheet if you are unable to collect a water sample and why.
 
San Diego Watershed Monitoring ProgramInstructions for Proper Use of Sampling Equipment 
A
Total of 6 Whirl-Pak
sampling bags are collected at each SamplingSite and stored on ice in the cooler. Remember to keep this coolershut at all times to maintain a cool, constant temperature.III.
Water Quality Analysis Checklist (Monitor in the shade if possible!)
Put on
gloves
to avoid contact with sample.
MeasureAir Temperaturein the shade with
thermometer
. RecordInstrument ID number and obtain 3 data results.
COLLECT (1) 500 ml (large) Whirl-Pak bag to use for WATERQUALITY ANALYSIS. ONCE THE ANALYSIS IS COMPLETEDYOU MANY DISCARD THIS BAG & ITS CONTENTS.
MeasureWater Temperaturewith
Digital Thermometer
. RecordInstrument ID number and obtain 3 data results.
MeasureDissolved Oxygenusing
DO Meter
. Record Instrument IDnumber and obtain 3 data results.
MeasurepHwith
pH meter
. Record Instrument ID number andobtain 3 data results.
MeasureElectrical Conductivity (EC)using
EC meter
. RecordInstrument ID number and obtain 3 data results. Note the units!
Discard sample bag & water
used to collect this field data (the 500ml Whirl-Pak).
Return all water monitoring kit (including all equipment), cooler andsix total samples from each site to the San Diego Coastkeeper trainingsite for analysis.
Wash your hands
thoroughly after water monitoring is complete. Feelfree to use the hand sanitizer provided as frequently as you feelnecessary.
Use new gloves at each site.2
 
 ATTENTION: Fill the sample bag up to the white line or top of the designatedlabeling area of the Whirl-Pak bag and allow for some air space. Air space is needed to allow the bacteria in the sample oxygen to stay alive.
 
San Diego Watershed Monitoring ProgramInstructions for Proper Use of Sampling Equipment 
IV.
Complete the Field Data Sheet (please print legibly)3
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