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SDCK / Total Phosphorous Standard Operating Procedure / revision 4/27/10
SDCK LAB/Total Phosphorous, Hach
TNT 843
Standard Operating Procedure
LR (0.05±1.50 mg/L PO
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±P) Ascorbic Acid Method - Method 10210
Phosphate (PO43-) is a natural and anthropogenic component of surface waters. It is a plantnutrient, and its presence can affect vegetation growth and thus affect aquatic habitats.
Color or turbidity in samples can cause high results. The digestion in the total phosphateprocedure usually destroys all color and turbidity and a sample blank is not required.
Recommended sample and reagent temperature is 15²25 °C (59²77 °F). Recommendedreagent storage temperature is 15²25 °C (59²77 °F).
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efer to data sheet to track progress throughout the procedure.
Check boxes and record times and temperatures.
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ecord any and all results, and make note of results that read under the detectionlimits or seem unusual.
Change pipette tips between samples to avoid cross contamination.
Label enough TNT test vials for each sample, and for duplicates and blanks
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SDCK / Total Phosphorous Standard Operating Procedure / revision 4/27/10
SDCK LAB/Total Phosphorous Hach
Method 10210 Total TNT 843 SOP (Continued)
Do not perform step 5 until all samples have been added to vials,keep aside and putALL samples into the reactor at same time.
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emember to process one sub-watershedbatch at a time after digestion (step 8)
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