SDCK / Ammonia Standard Operating Procedure / revision 4/27/10

SDCK LAB/Ammonia, Hach

TNT 830

Standard Operating ProcedureULR (0.015 to 2.000 mg/L NH

²N) Salicylate Method - Method 10205Ammonia is a natural component of marine sediments and common constituent ofmunicipal effluents and agricultural runoff. Ammonia may occur in concentrations that aretoxic to marine organisms in toxicity tests; therefore, it must be accurately measured in orderto rule out interference with toxicity test results.

Color or turbidity in samples can cause high results. Filtering of the sample eliminates color interference and a sample blank is not required.

Recommended sample and reagent temperature is 20²23 °C (68²73.4 °F). Incorrect resultsmay be obtained if test is not performed at the recommended temperature.Recommended reagent storage is 2²8 °C (35.6²46.4 °F).If immediate analysis is not possible, store at 4 °C (39 °F) if the sample is to be analyzed within24 to 48 hours.

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Refer to data sheet to track progress throughout the procedure.

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Check boxes and record times and temperatures.

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Record any and all results, and make note of result that read under the detection limits(UR, Under Range) or seem unusual.

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Change pipette tips between samples to avoid cross contamination.

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Label enough TNT test vials for each sample, and for duplicates and blanks

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Remember to process one sub-watershed batch at a time up to step 5