P. 1
Microscope Parts and Functions

Microscope Parts and Functions

|Views: 670|Likes:
Published by Irish Padlan Mejia

More info:

Published by: Irish Padlan Mejia on Aug 25, 2012
Copyright:Attribution Non-commercial


Read on Scribd mobile: iPhone, iPad and Android.
download as DOCX, PDF, TXT or read online from Scribd
See more
See less





Microscope Parts and Functions

A microscopes function is to see things at different levels, magnifications e.g. cells that can not be seen with a naked eye.

1. Eyepiece: The eyepiece (sometimes called the 'ocular') is the lens of the microscope closest to the eye that you look through. It is half of the magnification equation (eyepiece power multiplied by objective power equals magnification), and magnifies the image made by the objective lens... sometimes called the virtual image. Eyepieces come in many different powers. One can identify which power any given eyepiece is by the inscription on the eyecup of the lens, such as "5x", "10x", or "15X". Oculars are also designed with different angles of view; the most common is the wide field (W.F.). 2. Eyepiece Holder: This simply connects the eyepiece to the microscope body, usually with a set-screw to allow the user to easily change the eyepiece to vary magnifying power. 3. Body: The main structural support of the microscope which connects the lens apparatus to the base. 4. >Nose Piece: This connects the objective lens to the microscope body. With a turret, or rotating nose piece as many as five objectives can be attached to create different powers of magnification when rotated into position and used with the existing eyepiece. 5. Objective: The lens closest to the object being viewed which creates a magnified image in an area called the "primary image plane". This is the other half of the microscope magnification equation (eyepiece power times objective power equals magnification). Objective lenses have many designs and qualities which differ with each manufacturer. Usually inscribed on the barrel of the objective lens is the magnification power and the numerical aperture (a measure of the limit of resolution of the lens). 6. Focusing Mechanism: Adjustment knobs to allow coarse or fine (hundredths of a millimeter) variations in the focusing of the stage or objective lens of the microscope. 7. Stage: The platform on which the prepared slide or object to be viewed is placed. A slide is usually held in place by spring-loaded metal stage clips. More sophisticated high-powered microscopes have mechanical stages which allow the viewer to smoothly move the stage along the X (horizontal path) and Y (vertical path) axis. A mechanical stage is a must for high-power observing. 8. Illumination Source: The means employed to light the object to be viewed. The simplest is the illuminating mirror which reflects an ambient light source to light the object. Many microscopes have

Condenser lens Directs light to the object being viewed. Be sure you can identify each lens.) . Compound light microscope: Microscope Part Function Eyepiece (ocular lens) The part you look through. It has a lens that magnifies the object. 40x. For example. Most microscopes illuminate from underneath. you will adjust it to direct light through the lens. through the object. Turn it to change lenses. an electrical light source for easier and more consistent lighting.and high-power magnification to bring the object into sharper focus. Use it only with the low-power objective lens. Each lens has a different power of magnification. the low-power objective lens is usually 10x. Coarse-adjustment knob Moves the tube or stage up or down and brings the object into focus. Diaphragm Use this to control the amount of light reaching the object being viewed. Fine-adjustment knob Use with medium. On the other hand. such as 10x. stereo microscopes use both top and bottom illumination. Objective lenses Magnify the object.9. The magnifying power is engraved on the side of the eyepiece.The magnifying power is engraved on the side of each objective lens. 10. If it does. Clips hold the slides into position. usually by ten times (10x). Each lens clicks into place. A hole in the center of the stage allows the light from the light source to pass through the slide. Tube Holds the eyepiece and the objective lenses at the proper working distance from each other. and 100x. Base: The bottom or stand upon which the entire microscope rests or is connected. Generally electrical light sources are either tungsten or fluorescent. Stage Supports the microscope slide. Light source Shining a light through the object being viewed makes it easier to see the details. to the objective lens. the fluorescent being preferred because it operates at a cooler temperature. Revolving nosepiece Rotating disk holds two or more objective lenses. (Your microscope might have a mirror instead of a light.

You're Reading a Free Preview

/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->