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20600 008041-3 05/01

For in vitro diagnostic use

Identification system for streptococci


API 20 STREP is a standardized method combining 20 biochemical tests that offer widespread capabilities. It enables group or species
identification of most streptococci encountered in medical bacteriology. The complete list of those organisms that it is possible to
identify with this system is given in the Identification Table at the end of this package insert.

PRINCIPLE COMPOSITION OF MEDIA AND REAGENTS


The API 20 STREP strip consists of 20 microtubes containing Suspension Demineralized water
dehydrated substrates for the demonstration of enzymatic activity Medium
2 ml
or the fermentation of sugars. GP Medium Cystine 0.5 g
The enzymatic tests are inoculated with a dense suspension of 2 ml Tryptone 20 g
organisms, made from a pure culture, which is used to rehydrate Sodium chloride 5g
Sodium sulfite 0.5 g
the enzymatic substrates. The metabolic end products produced Phenol red 0.17 g
during the incubation period are either revealed through Demineralized water to make 1000 ml
pH : 7.8
spontaneous colored reactions or by the addition of reagents.
NIN reagent Ninhydrin 7g
The fermentation tests are inoculated with an enriched medium 5 ml 2-methoxyethanol 100 ml
which reconstitutes the sugar substrates. Fermentation of TOXIC
carbohydrates is detected by a shift in the pH indicator. R60 : May impair fertility.
The reactions are read according to the Interpretation of R61 : May cause harm to the unborn child.
R10 : Flammable.
Reactions (Table 2) and the identification is obtained by referring R20/21/22 : Harmful by inhalation, in contact with skin
to the Analytical Profile Index or using the identification software. and if swallowed.
S53 : Avoid exposure (avoid contact with skin and
eyes, vapor inhalation and brutal superheating).
S45 : In case of accident or if you feel unwell, seek
REAGENTS medical advice immediately (show the label where
Kit contents (25 tests) : possible).
Voges- 40% aqueous KOH solution 89%
- 25 API 20 STREP strips Proskauer in demineralized water 11%
- 25 incubation boxes reagent: CORROSIVE
Potassium
- 25 ampules of GP Medium hydroxide R35 : Causes severe burns.
S24/25: Avoid contact with skin and eyes.
- 25 result sheets 30 ml
S26 : In case of contact with eyes, rinse immediately
- 25 swabs with plenty of water and seek medical advice.
- 1 package insert S36/37/39 : Wear suitable protective clothing, gloves
and eye/face protection.
S45: Incase of accident or if you feel unwell, seek
Additional products (not included in the kit) : medical advice immediately (show the label where
possible).
- Suspension Medium, 2 ml (Prod. No. 70700)
- Reagents : Ninhydrin (NIN) (Prod. No. 70490) Voges alpha-naphthol 6g
Proskauer Ethyl alcohol 100ml
Potassium hydroxide (Prod. No. V7053) reagent: VP2
VP2 or (Prod. No. 70430) 5 ml or
_-naphthol (Prod. No. V7054) alpha-naphthol 2.05 g
alpha-naphthol to be diluted with 95% ethyl alcohol 29ml
ZYME A (Prod. No. 70470) 30 ml
ZYME B (Prod. No. 70480) FLAMMABLE AFTER RECONSTITUTION
- Mineral oil (Prod. No. 70100) R21/22: Harmful in contact with skin and if swallowed.
- Sterile Pasteur pipettes S24/25: Avoid contact with skin and eyes.
- McFarland Standard #4 (Prod. No. 70900) ZYME A reagent Tris-hydroxymethyl-aminomethane 25 g
8 ml Hydrochloric acid (37 %) 11 ml
- API 20 STREP Analytical Profile Index (Prod. No. 20690) or Sodium lauryl sulfate 10 g
identification software (consult bioMérieux) H2 O 100 ml
- Ampule rack (Prod. No. 70200) ZYME B reagent Fast Blue BB 0.35 g
- Columbia blood agar plates 8 ml 2-methoxyethanol 100 ml
- Schaedler broth (optional) TOXIC
R60 : May impair fertility.
R61 : May cause harm to the unborn child.
Required laboratory equipment : R10 : Flammable.
R20/21/22 : Harmful by inhalation, in contact with skin
- 35-37°C incubator and if swallowed.
S53 : Avoid exposure - obtain special instructions
- Refrigerator (2-8°C) before use.
S45 : In case of accident or if you feel unwell, seek
- Bunsen burner medical advice immediately (show the label where
- Marker pen possible).
- Anaerobic jar
- Inoculating loop
STORAGE OF THE STRIPS AND MEDIA
4. Alpha-naphthol :
The strips and media should be stored at 2-8°C until the
expiration date indicated on the packaging. • Reconstitute with 29 ml of 95% ethanol.
• Carefully close the bottle.
STORAGE OF THE REAGENTS • Shake.
The reagents should be stored in the dark at 2-8°C (except • Reagent can be used after active ingredient is completely
Potassium hydroxide and ZYME A which can be stored at 2- disolved.
30°C) until the expiration date indicated on the packaging. VP2 • Dispense one drop of reagent.
may be kept up to 1 month after the ampules have been opened • Carefully close the bottle after use an store it as indicated in the
and the reagent transferred to the dropper-bottle. After paragraph "Storage of the reagents".
reconstitution, alpha naphthol should be stored at 2-8°C and may
be kept for up to 90 days, (or until the expiration date if this
comes first) : record the reconstitution date on the bottle label.
The ZYME A, ZYME B and NIN reagents may be kept for up to 1 WARNINGS AND PRECAUTIONS
month after the ampules have been opened and the reagents • For in vitro diagnostic use only.
transferred into the dropper-bottles, (or until the expiration date if • Qualified laboratory personnel should use aseptic technique
this comes first) : record the date opened on the bottle label. and established precautions for infectious agents.
The NIN and ZYME B reagents are very sensitive to
light : wrap the bottles in aluminum foil and only leave them out of • Do not pipette specimens or reagents by mouth.
the refrigerator while being used. Do not leave them on the bench • Do not use reagents past the expiration date.
for prolonged periods of time. • Do not allow reagents to come into contact with skin, eyes or
The NIN reagent is very sensitive to traces of water and air : clothing.
transfer the reagent into the dropper-bottle using a dry pipette • Do not interchange reagents or consumables between different
and keep the bottle tightly closed. lot numbers.
The ZYME B reagent is normally yellow in color. Dispose of the • Upon removal from refrigerator, allow reagents to come to
reagent if any tint of pink (sign of deterioration) is observed. room temperature (20-30°C) before using.
At 2-8°C, the ZYME A reagent may form a precipitate which does
not affect any of the properties of the reagent and which may be • Open ampules carefully as follows :
redissolved by gently heating. - Hold the ampule in one hand in a vertical position
(white plastic cap uppermost).
- Press the cap down as far as possible.
USE OF THE REAGENTS - Cover the flattened part of the cap with the upper
Allow reagents to come to room temperature (20-30°C) before part of the thumb.
using. - Apply thumb pressure in an outward motion to
the flattened part of the cap to snap off the top of
1. ZYME A and ZYME B reagents : the ampule inside the cap.
*For ampule with no dropper-cap :
• Open the ampule of reagent as indicated in the paragraph - Carefully remove the cap.
"Warnings and Precautions" (ampule with dropper-cap). *For ampule with dropper-cap :
• Dispense one drop of reagent. - Turn the ampule upside down and maintain it in
• Carefully close the bottle after use and store it as indicated in a vertical position.
the paragraph "Storage of the reagents". - Squeeze on the cap to transfer all the reagent
into the dropper-bottle.
2. NIN reagent : • All inoculated products should be considered infectious and
handled appropriately.
• Open the ampule of reagent as indicated in the paragraph
• All patient specimens and microbial cultures are potentially
"Warnings and Precautions" (ampule with no dropper-cap).
infectious and should be treated with universal precautions
• Take up the contents of the ampule using a completely dry (NCCLS M29-A: Protection of Laboratory Workeres from
pipette and transfer this liquid into the dropper-bottle. Instrument Biohazards and Infectious Disease Transmitted by
• Fit the dropper to the bottle. Blood, Body Fluids, and Tissue: Approved Guideline. 1997).
• Dispense one drop of reagent. • After completing test, reading and interpretation, all specimens,
• Carefully close the bottle after use and store it as indicated in spills and inoculated products must be autoclaved, incinerated
the paragraph "Storage of the reagents". or immersed in a germicide prior to disposal.
• Interpretation of the test results should be made by a
3. VP2: competent microbiologist who should also take into
consideration the patient history, the source of the specimen,
• Open the ampule of reagent as indicated in the paragraph colonial and microscopic morphology and, if necessary, the
Warnings and Precautions" (ampule with dropper cap). results of any other tests performed, particularly the
• Dispense one drop of reagent. antimicrobial susceptibility patterns.
• Carefully close the bottle after use an store it as indicated in the • Any changes or modifications in the procedure may affect the
paragraph "Storage of the reagents". results.
INSTRUCTIONS FOR USE • Using a swab, harvest all the culture from the previously
Specimens and bacterial cultures should be considered infectious prepared subculture plate. Make a dense suspension with a
and handled appropriately by trained and competent technicians. turbidity greater than 4 McFarland.
Aseptic technique and usual handling precautions for the
bacterial group studied should be observed throughout this Inoculation of the strip
procedure ; refer to Universal Precautions (NCCLS M29-A: • In the first half of the strip (tests VP to ____ ADH) distribute the
Protection of Laboratory Workers from Instrument Biohazards suspension with a sterile pipette, avoiding the formation of
and Infectious Diseases Transmitted by Blood, Body Fluids, and bubbles (tilt the strip slightly forwards) :
Tissue : Approved Guideline. 1997). - For the tests VP to LAP : distribute approximately 100 µl into
For additional handling precautions, refer to Biosafety in each cupule (3 drops with a Pasteur pipette ).
Microbiological and Biomedical Laboratories, HHS Publication - For the ____
ADH test : fill the tube only.
No. (CDC) 93-8395, 3rd Edition (May 1993), or to the regulations • In the second half of the strip (tests RIB
___ to ______
GLYG ) :
of each country. - Open an ampule of GP Medium as indicated in the
paragraph "Warnings and Precautions" (ampule with no
Selection of colonies dropper-cap) and transfer the rest of the suspension into it
Once the microorganism to be identified has been isolated and (appr. 0.5 ml). Mix well.
verified to be a member of the genus Streptococcus or related - Distribute this new suspension into the tubes only.
genera shown in Table 3 (Gram, catalase test) : • Fill the cupule of the underlined tests (ADH
____ to ______
GLYG ) with
• Note the type of hemolysis on the result sheet mineral oil to form a convex meniscus.
(21st test). • Place the lid on the tray.
• Pick a well-isolated colony (Note 1) and suspend it in 0.3 ml of • Incubate at 35-37°C for 4 hours to obtain a first reading and for
sterile water. Homogenize well. 24 hours to obtain a second reading if this is required.
• Flood a Columbia sheep blood agar plate (Note 2) with this
suspension (or aseptically swab the entire surface of the agar). Reading of the strip
• Incubate anaerobically for 18-24 hours at 35-37°C. After 4 hours of incubation :
NOTE 1 : Alpha-hemolytic streptococci and enterococci produce • Add the reagents :
sufficiently large colonies after 24 hours of incubation. For other - VP Test : 1 drop of each of Potassium hydroxide and VP2 or
streptococci, it is preferable to select a colony after 48 hours of alpha-naphthol.
incubation. For fastidious strains (producing minute colonies after - HIP Test : 2 drops of NIN reagent.
48 hours), the following procedure is recommended : - PYRA, _GAL, _GUR, _GAL, PAL and LAP Tests : 1 drop of
- Culture the colony in 1 ml of Schaedler broth at 35-37°C for 5 each of ZYME A and ZYME B reagents.
hours.
- Flood a Columbia sheep blood agar plate with the entire • Wait 10 minutes, then read the reactions by referring to the
culture. Remove any excess liquid. Interpretation of Reactions Table (Table 2). If necessary,
- Incubate anaerobically for 18 hours at 35-37°C. expose the strip to a strong light (10 seconds with a 1000 W
lamp) to decolorize any excess reagents in the tubes PYRA to
NOTE 2 : In the case of suspected pneumococci, it is advisable LAP.
to prepare 2 plates in order to obtain sufficient growth.
Reincubation is necessary :
Preparation of the strip - if the profile cannot be found in the API 20 STREP Analytical
Profile Index
• Prepare an incubation box (tray and lid) and distribute about 5 - if the profile is given with the following note :
ml of distilled water or demineralized water [or any water
IDENTIFICATION NOT VALID
without additives or chemicals which may release gases (e.g.,
BEFORE 24 HOURS OF INCUBATION
Cl2, CO 2, etc.)] into the honeycombed wells of the tray to create
After 24 hours, reread the reactions ESC, ____ ADH, and RIB
___ to
a humid atmosphere.
GLYG . Do not reread the enzymatic reactions (HIP, PYRA,
______
• Record the strain reference on the elongated flap of the tray. _GAL, _GUR, _GAL, PAL, LAP) and VP. Record the reactions on
• Remove the strip from its packaging and place it in the tray. the result sheet.

Preparation of the inoculum


• Open an ampule of Suspension Medium (2 ml) as indicated in
the paragraph "Warnings and Precautions" (ampule with no
dropper cap) or use any tube containing 2 ml of distilled water
without additives.
Identification
Identification can be obtained :
• using the Analytical Profile Index : the pattern of the reactions
obtained must be coded into a numerical profile.
On the result sheet, the tests are separated into groups of 3 5 240 550 / 5 240 770 Streptococcus mutans
and a number 1, 2 or 4 is indicated for each. By adding the
numbers corresponding to positive reactions within each NOTE : The hemolytic reaction constitutes the 21st test ; beta-
group, a 7-digit profile number is obtained. hemolysis is considered as positive with a numerical value of 4.
All other hemolytic reactions are considered as negative with a
To obtain information on any profile not listed in the codebook, numerical value of 0. Nevertheless, this test may be of
call the Voice Response System at 800-645-7056. discriminant value for the identification of certain species.
• using the identification software.

QUALITY CONTROL
The media, strips, and reagents are systematically quality controlled at various stages of their manufacture. For those who wish to
perform their own quality control tests with the strip, it is recommended that the following stock cultures be used, to obtain the results
below : Table 1

VP HIP ESC PYRA _GAL _GUR _ GAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG
1. – – – – – + – + + + + – – + + – – – + +
2. + V + + + V + – V + + + + – + + + + + –
3. + + + V V + – V + + + – + + + + + + – –
4. V V + V V – – – – – – – + – – + – + + –

1. Streptococcus equi ssp zooepidemicus ATCC 700400 3. Streptococcus uberis ATCC 700407
2. Enterococcus gallinarum ATCC 700425 4. Aerococcus viridans ATCC 700406
ATCC : American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110-2209, USA.
• Inoculum adjusted to between 4.5 and 5.5 McF.
• Profiles obtained after : - 4 hours of incubation for tests VP to LAP
- 24 hours of incubation for tests ____
ADH to _____
GLYG
• Strains cultured on Columbia sheep blood agar

DISPOSAL OF USED MATERIAL LIMITATIONS


After use, all ampules, swabs, pipettes, strips and incubation The API 20 STREP system is intended uniquely for the
boxes should be autoclaved, incinerated or immersed in a identification of those species included in the database (see
disinfectant for decontamination prior to disposal. Identification Table (Table 3) at the end of this package insert). It
cannot be used to identify any other microorganisms or to
exclude their presence.

RANGE OF EXPECTED VALUES


The API 20 STREP test produces color reactions which make
positive and negative identification possible. Absolute values are
not obtained.
INTERPRETATION OF REACTIONS

Table 2
TESTS SUBSTRATES REACTIONS/ENZYMES RESULTS

NEGATIVE POSITIVE

Potassium hydroxide(1 drop) +VP2


or alpha-naphthol (1drop) / wait 10
min

VP Pyruvate Acetoin production Colorless Pink-Red (3)

NIN (2 drops) / wait 10 min

HIP Hippurate Hydrolysis Colorless/Pale blue Dark blue/Violet

4 hrs. 24 hrs. 4 hrs. 24 hrs.

ESC Esculin _-glucosidase Colorless Colorless Black Black


Pale yellow Pale yellow Grey
Light grey

ZYME A (1 DROP) + ZYME B (1 DROP) / 10 MIN (PYRA to LAP) (1)


if necessary, decolorize with intense light

PYRA Pyrrolidonyl-2-naphthylamide Pyrrolidonyl arylamidase Colorless or very pale orange Orange

_GAL 6-Bromo-2-naphthyl
_-D-galactopyranoside _-galactosidase Colorless Violet

_GUR Naphthol AS-BI


_-D-glucuronate _-glucuronidase Colorless Blue

_GAL 2-naphthyl- _-D-


galactopyranoside _-galactosidase Colorless or very pale violet Violet

PAL 2-naphthyl phosphate Alkaline phosphatase Colorless or very pale violet Violet

LAP L-leucine-2-naphthylamide Leucine arylamidase Colorless Orange

ADH
____ Arginine Arginine dihydrolase Yellow Red

4 hrs. 24 hrs. 4 hrs. 24 hrs.

RIB
___ Ribose Acidification Red Orange/Red Orange/Yellow Yellow
ARA
____ L-Arabinose Acidification Red Orange/Red Orange/Yellow Yellow
MAN
____ Mannitol Acidification Red Orange/Red Orange/Yellow Yellow
SOR
____ Sorbitol Acidification Red Orange/Red Orange/Yellow Yellow
LAC
____ Lactose Acidification Red Orange/Red Orange/Yellow Yellow
TRE
____ Trehalose Acidification Red Orange/Red Orange/Yellow Yellow
INU
___ Inulin Acidification Red Orange/Red Orange/Yellow Yellow
RAF
____ Raffinose Acidification Red Orange/Red Orange/Yellow Yellow
AMD
____ Starch (2) Acidification Red Orange/Red Orange/Yellow Yellow

GLYG
_____ Glycogen Acidification Red or Orange Bright yellow

(1) During a second reading after 24 hours of incubation, a deposit may be noticed in the tubes where the ZYME A and ZYME B reagents have been added.
This phenomenon is normal and should not be taken into consideration.

(2) The acidification of starch is frequently weaker than that of other sugars.

(3) A pale pink color obtained after 10 minutes should be considered negative.
0
api 20 Strep 07625 B - 08/97

RECOMMENDED METHODOLOGY

- Cocci
- Gram +
- Catalase −

Blood agar

Columbia blood agar

24:00 02 37°C

> 4 McF

Suspension Medium 2 ml

VP ADH

ADH

GP Medium

RIB GLYG

RIB GLYG

4:00 37°C

24:00 37°C VP : Potassium hydroxide +


VP2 or alpha naphthol
HIP : NIN
api 20 STREP PYRA LAP : ZYME A + ZYME B

+ - + - + -
IDENTIFICATION TABLE
Table 3
% of positive reactions after 4/24 hrs. at 35-37°C

API 20 STREP V6.0 VP HIP ESC PYRA AGAL BGUR BGAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG HEM

Aerococcus viridans 1 13 50 96 54 33 16 37 1 5 1 83 33 85 70 83 99 33 41 70 33 1

Aerococcus viridans 2 15 70 50 76 10 20 25 1 5 5 25 1 35 2 70 89 1 5 24 1 5

Aerococcus viridans 3 22 88 99 40 85 48 14 14 1 1 8 2 82 5 91 99 37 99 14 1 1

Alloiococcus otitis 0 25 0 100 0 3 100 1 90 0 0 0 0 0 0 20 0 0 0 0 0

Enterococcus avium 99 60 99 94 15 0 24 1 99 0 99 40 100 95 95 99 1 40 15 0 1

Enterococcus durans 100 43 100 97 32 2 76 0 91 100 99 15 2 0 84 76 0 0 56 0 18

Enterococcus faecalis 99 46 99 97 1 0 21 4 99 94 98 0 98 92 92 100 0 0 96 2 1

Enterococcus faecium 94 43 99 95 42 1 90 3 97 93 85 70 78 18 84 98 26 9 73 3 1

Enterococcus gallinarum 99 99 100 100 95 45 99 0 99 99 99 100 99 1 100 100 99 99 83 20 0

Gardnerella vaginalis 0 95 0 0 0 1 53 0 99 0 46 6 1 0 1 0 0 0 73 53 0

Gemella haemolysans 25 0 0 70 0 0 1 84 40 1 1 0 20 10 5 2 0 0 10 5 1

Gemella morbillorum 3 0 0 35 0 0 10 35 86 4 5 0 1 0 1 11 3 1 16 5 0

Lactococcus lactis ssp cremoris 98 15 41 1 13 0 41 4 89 0 27 0 17 0 96 30 0 20 25 0 0

Lactococcus lactis ssp lactis 90 40 99 35 3 0 35 3 96 95 95 15 45 1 72 87 4 5 90 3 1

Leuconostoc spp 91 1 60 5 55 0 65 2 70 10 37 35 29 4 35 65 0 42 11 0 0

Listeria spp 97 79 98 0 0 0 0 0 85 0 6 0 0 0 49 92 1 1 72 0 26

Abiotrophia adiacens 0 0 10 80 0 25 0 0 99 0 0 0 0 0 0 0 0 0 0 0 0

Abiotrophia defectiva 25 0 15 99 100 0 100 0 92 0 0 0 0 0 99 100 5 93 99 0 0

Streptococcus acidominimus 1 95 4 13 1 66 30 60 96 18 17 0 42 10 70 65 0 0 10 0 0

Streptococcus agalactiae 100 99 1 1 4 79 1 96 99 99 98 0 1 1 50 87 0 1 35 4 75

Streptococcus anginosus 100 0 100 0 44 0 1 99 100 100 0 0 33 0 99 88 0 44 97 0 37

Streptococcus bovis I 97 2 100 2 71 4 14 0 97 0 2 13 86 0 100 90 63 90 100 90 0

Streptococcus bovis II 1 95 4 97 1 86 1 17 0 100 1 0 14 0 0 93 30 61 99 73 65 0

Streptococcus bovis II 2 86 4 100 13 85 88 94 0 100 13 0 1 0 0 99 99 13 72 40 13 0

Streptococcus canis 0 1 25 4 95 1 80 100 100 100 100 0 0 0 99 1 0 1 99 0 100

Streptococcus constellatus 100 1 27 0 0 0 5 99 100 100 0 0 0 0 10 72 0 0 12 0 61

Streptococcus dys.ssp dysgalactiae 0 0 1 1 1 99 0 100 99 100 99 0 1 50 86 100 0 1 99 30 2

Streptococcus dys.ssp equisimilis 0 1 25 1 1 99 1 99 100 97 97 1 1 1 45 99 0 1 98 40 94

Streptococcus equi ssp equi 1 0 1 0 0 100 0 100 100 100 0 0 0 0 0 1 0 0 100 100 100

Streptococcus equi ssp zooepidemicus 0 1 15 0 0 100 1 99 100 99 85 0 0 99 100 0 0 0 99 99 99

Streptococcus equinus 100 0 95 0 28 0 1 1 100 0 0 0 30 0 25 7 25 15 17 10 0

Streptococcus group L 0 75 1 0 0 100 1 100 100 100 100 0 0 0 75 100 0 0 100 98 94

Streptococcus intermedius 100 0 87 0 0 0 44 99 100 100 0 0 0 0 99 99 3 3 99 0 40

Streptococcus mitis 1 1 0 3 1 21 0 25 35 99 19 14 1 0 1 94 7 3 26 67 5 0

Streptococcus mitis 2 0 0 3 0 31 0 35 50 100 99 1 0 1 0 100 1 1 31 84 0 0

Streptococcus mutans 99 0 99 1 64 0 1 1 100 18 0 0 99 90 90 100 81 81 1 0 1

Streptococcus oralis 0 0 1 1 50 0 46 72 100 5 1 0 1 0 99 32 1 72 96 0 0

Streptococcus pneumoniae 0 0 39 60 70 3 79 3 100 57 3 0 0 0 99 98 64 87 84 10 1

Streptococcus porcinus 100 5 99 1 19 99 1 97 97 100 98 0 88 88 83 99 0 0 50 0 100

Streptococcus pyogenes 0 1 5 98 0 15 0 100 100 99 0 0 8 1 99 98 0 1 61 22 98

Streptococcus salivarius ssp salivarius 85 0 98 1 8 0 70 20 100 0 0 0 5 1 86 67 34 88 74 1 1

Streptococcus sanguis 0 1 42 0 63 0 1 5 100 90 0 0 1 48 83 98 33 55 67 0 0

Streptococcus suis I 0 1 82 53 80 94 76 1 100 91 0 0 7 0 94 100 75 0 100 89 0

Streptococcus suis II 0 1 70 41 91 91 52 3 100 95 0 0 3 1 99 98 63 93 99 96 2

Streptococcus uberis 99 98 100 35 10 86 5 30 100 98 99 0 99 98 99 100 89 10 50 20 0


BIBLIOGRAPHY

1. APPELBAUM P.C., CHAURUSHIYA P.S., JACOBS M.R., 6. HUMAN R.P. and TILLOTSON G.S.
DUFFETT A. Identification of Gardnerella vaginalis with the API 20 Strep
Evaluation of the Rapid Strep System for Species System.
Identification of Streptococci. (1985) J. Clin. Microbiol., 21, 985-986.
(1984) J. Clin. Microbiol., 19, 588-591. 7. KLOOSTERMAN R.E., CULLEN K.D.
2. BALL L.C., COLMAN G. Comparison of Two Commercial Systems for the Rapid
A Comparison of Conventional Methods and API Galleries Identification of Streptococci.
for the Identification of Streptococci. (1984) ASM ST. LOUIS C198.
(1982) International Meeting on Streptococci and 8. MacGOWAN A.P., MARSHALL R.J., REEVES D.S.
Streptococcal Diseases, LUND SWEDEN, 41-42. Evaluation of API 20 STREP System for Identifying Listeria
3. BANNISTER M.F., BENSON C.E. and SWEENEY C.R. Species.
Rapid Species Identification of Group C Streptococci (1989) J. Clin. Path., 42, 548-550.
Isolated from Horses. 9. RUOFF K.L., KUNZ L.J.
(1985) J. Clin. Microbiol., 21, 524-526. Use of the Rapid STREP System for Identification of
4. COLMAN G., BALL L.C. Viridans Streptococcal Species.
Identification of Streptococci in a Medical Laboratory. (1983) J. Clin. Microbiol., 18, 1138-1140.
(1984) J. Appl. Bact., 57, 1-14. 10. TILLOTSON G.S.
5. FACKLAM R.R., RHODEN D.L., SMITH P.B. An Evaluation of the API 20 Strep System.
Evaluation of the Rapid Strep System for the Identification (1982) J. Clin. Path., 468-471.
of Clinical Isolates of Streptococcus Species.
(1984) J. Clin. Microbiol., 20, 894-898.
Fabricant / Manufacturer bioMérieux SA

bioMérieux SA bioMérieux, Inc.


au capital de 77 421 420 F 595 Anglum Road Hazelwood,
673 620 399 RCS LYON MO 63042-2320 / USA
69280 Marcy-l’Etoile / France tel. (1) 314-731-8500 / fax (1) 314-731-8800
tel. (33) 4 78 87 20 00 / fax (33) 4 78 87 20 90 Imprimé en / Printed in USA