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CALCULATION FORMULA FATTY ACID METHYL ESTER (FAME) YIELD

WC_nDecane = WnDecane_onSampleTest x WC_nDecaneMolecule Where: WC_nDecane = Weight of Carbon (C) in n-Decane sample test (mg) WnDecane_onSampleTest = Weight of n-Decane sample test (mg) WC_nDecaneMolecule = Weight of C in n-Decane Molecule (%) WC_ME = AME / AnDecane x WC_nDecane Where: WC_ME AME AnDecane = Weight of C of Methyl Ester in the sample test (mg) = Peak area of Methyl Ester by GC = Peak area of n-Decane by GC

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WME = WC_ME / WC_MEmolecul x MW_ ME Where: WME = Weight of Methyl Ester in the sample test (mg) WC_MEmolecul = Weight of C in 1 molecule Methyl Ester (mg/mol) MW_ ME = Molecular Weight of Methyl Ester (mg/mol) WME_insampletest Where: WME_insampletest Wsampletest = WME / Wsampletest x 100 % = Weight of Methyl Ester in the sample test (%) = Weight of sample test for GC (mg)

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