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2012 JOURNAL OF BIOMEDICAL SCIENCES
Vol. 1 No. 4:1 doi: 10.3823/1009

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Relationship between cigarette smoking and novel risk factors for cardiovascular disease

B Raghu1 and P Venkatesan2
1    epartment of Biochemistry, D International Medical School, Shah Alam, Malaysia. 2 Department of BioStatistics,Tuberculosis Research Center, Chennai-600031, India. .Correspondence:

 vasuraghu@hotmail.com
Dr. B.Raghu, X – 6, 5th Main Road, Anna Nagar, Chennai 600040. India

Abstract 
Background & Objectives: The aim of the present study was to assess the association between cigarette smoking and the alteration of plasma concentration of cardiovascular markers.

Methods: Twenty male cigarette smokers and 20 healthy age matched male non
smokers were included in the study. Plasma levels of fasting cholesterol, triglycerides, High Density Lipoprotein(HDL), Low Density Lipoprotein(LDL), Very Low Density Lipoprotein(VLDL) , C- reactive protein and homocysteine were estimated. Results Both serum total homocysteine and C-reactive protein levels were significantly increased in cigarette smokers than in non-smokers(p<0.001). Fasting serum levels of total cholesterol, triglycerides , LDL, VLDL and total cholesterol / HDL ratio were observed to be significantly higher in smokers than non-smokers(p<0.001). However,fasting HDL concentration was significantly decreased in smokers than non-smokers(p<0.001). In conclusion, our study identifies a strong ,positive relationship between cigarette smoking and elevated levels of all three novel risk factors for cardiovascular disease.

Interpretation & Conclusion: These findings suggest that inflammation and
hyperhomocysteinemia may be important mechanisms by which smoking promotes atherosclerotic disease

This article is available from: www.jbiomeds.com

Key words: Smoking, Homocysteine, Lipid profile, C-reactive protein.

Introduction
Smoking remains the single most common cause of preventable deaths. Each year, over 430,000 people die as a result of a smoking related disease(1,2).Cigarette smokers have a higher risk of coronary artery disease than non-smokers. Several possible explanations have been offered for this association, including altered blood coagulation (3), impaired integrity of the arterial wall (4), changes in blood lipid and lipoproteins concentrations (5), elevated plasma levels of homocysteine (6,7,8) and elevated plasma C-Reactive protein levels(9).Cigarette smoking is known to be associated with a raised plasma homocysteine level (10-13). Cigarette smoking © Copyright iMedPub

also leads to increase in the concentration of serum lipids (1417) Smoking has been associated with increased CRP levels, possibly caused by the tissue damaging effects of tobacco smoke (18,19). In contrast to the information on cigarette smoking, few studies have examined the relationship between cigarette smoking and novel risk factors for cardiovascular disease in a general population (20,21). To understand further the health risks associated with cigar smoking, we examined the association between cigarette smoking and the alteration of plasma concentration of cardiovascular markers.

1

001 2 © Copyright iMedPub .smokers Smokers p-value * Not Significant 20 20 Table 2.80 ± 13. Subject  n Total Cholesterol  Triglyce-rides  (mg%) (mg%)  HDL (mg%) LDL (mg%) VLDL (mg%) Total Cholesterol/ HDL Ratio Non -smokers Smokers p-value 20 20 163. serum concentration of total homocysteine was determined by the fluorescence polarization immunoassay(Abbott Axsym. 4:1 doi: 10.75 ± 5.60 29.24 6.25 ± 5.81 40.001 50.48) were included in the study.28 ± 9 .72) and twenty male cigarette smokers aged between 26-58 years (mean± SD: 36.001 120. The results are expressed as Mean ± SD for all the parameters and the statistical significance of differences among groups was examined by using t-test. The logistic regression analysis also showed significant changes in all the parameters in smokers when compared to non-smokers.35 ± 6. Effect of Cigarette Smoking on Lipid Profile.68 <0. BN-100).25 ± 7. VLDL and total cholesterol/HDL ratio were observed to be significantly higher in smokers (p<0. However. Fasting serum levels of total cholesterol.001 3.15 <0.23 <0.80 171.France).001) than non-smokers.001 24. Table 1. LDL .25 ± 7. LDL. VLDL-cholesterol was calculated from measured triglycerides divided by 5. Effect of cigarette smoking on serum lipids and lipoproteins are reported in Table 2.3823/1009 Our Site: http://www.06 < 0.08 ± 1. diabetes .iMedPub Journals 2012 JOURNAL OF BIOMEDICAL SCIENCES Vol. and triglyceride concentrations were quantified enzymatically with an autoanayzer(Maxmat PL analyzer. Age ( Years) 35.14 ± 0.41 ± 38.48 NS* Homocyteine ( µmol/L) 9.94 241.75 ± 26.07 ± 2. Serum was used to analyze total cholesterol.85 10.cholesterol were calculated by using the Friedewald equation(22). samples were centrifuged.35 ± 6. Effect of Cigarette Smoking on Homocyteine and C-Reactive Protein(values are mean ± SD). All the subjects were nonalcoholic and no history of cardiovascular disease.30 ± 2.39 27.001 C-Reactive Protein (mg %) 5.72 36.001 89. 1 No.50 < 0. HDL cholesterol.03 <0.001 Subject  n Non .75 ± 2.80 ± 35.001). Ten ml of fasting blood was collected by venipuncture into evacuated tubes that were free of anticoagulant to clot.30 ± 0.89 148.com/ Material and Methods  Twenty non smoking healthy male subjects aged between 27-48 years(mean ± SD:35.64 <0. Results As shown in Table 1 .other systemic and metabolic diseases and also they were from similar socioeconomic status. fasting HDL concentration was significantly decreased in smokers than non-smokers (p<0.35 ± 10.81 ± 1.001).imedpub. The logistic regression analysis was carried out to find the significance of each risk factor after adjusting for other factors.30 ± 12. triglycerides .12 <0.USA) and serum C-Reactive Protein (CRP) concentration was determined by means of nephelometry method( Dade-Behring. both serum total homocysteine and C-reactive protein levels were significantly increased in cigarette smokers than in non-smokers(p<0.

There are approximately 4000 chemicals in cigarettes. Tracy and colleagues(26) reported that pack-years of smoking but not current cigarette smoking was positively associated with log C-reactive protein levels among 400 health elderly participants in the cardiovascular health study. deadly.3823/1009 Our Site: http://www. such as C-reactive protein and fibrinogen .iMedPub Journals 2012 JOURNAL OF BIOMEDICAL SCIENCES Vol.com/ Discussion Few studies have demonstrated that elevated levels of C-reactive protein. Serum homocysteine level has also been related to increased risk for cardiovascular disease(24. This results provide further insight into the role that cigarette smoking may play in the development of atherosclerosis.positive relationship between cigarette smoking and elevated levels of all three novel risk factors for cardiovascular disease. reducing risks for diseases caused by smoking and improving health in general. The effects of smoking on human health are serious and in many cases. 4:1 doi: 10. Our study reports that all the three novel risk factors for cardiovascular disease. have been implicated in the pathogenesis of atherosclerosis(23). hundreds of which are toxic. The ingredients in cigarettes affect everything from the internal functioning of organs to the efficiency of the body’s immune system. These findings suggest that inflammation and hyperhomocysteinemia may be important mechanisms by which smoking promotes atherosclerotic disease. C-reactive protein and homocysteine were elevated in cigarette smokers when compared with non-smokers. © Copyright iMedPub 3 . serum lipids .25). Quitting smoking has immediate as well as long-term benefits. lipids and homocysteine are positively associated with risk for coronary heart disease and stroke. In conclusion. our study identifies a strong . In addition. Increased levels of inflammatory mediators .imedpub. 1 No.

Chest 2009. Microbiology.   6. CRC Press. Jessop W J. Metz C N. Biotechnology. Bartscherer D. Bernstein S L. Hanganu S C. Peter H W. Pawlicki K. CRP levels are elevated in smokers but unrelated to the number of cigarettes and are decreased by long-term smoking cessation in male smokers. Join Now! http://medicalia.imedpub. Stampter M J. Gordan D O.org Where Doctors exchange clinical experiences. Didilescu A C. 306: 103-109. Zielinska-Danch W. Puiy L. Europian Heart Journal 2005. Goldenberg R L. Effects of tobacco smoke and its constituents on lipid and carbohydrate metabolism. Scott C W. Cliver S P. N Engl J Med 1997. Boushey C J. Ridker P M.iMedPub Journals 2012 JOURNAL OF BIOMEDICAL SCIENCES Vol. Swierszcz J. practice guidelines will also be considered for publication. Mackenize T D. 17: 2167-2176.  10.  12. 1980. review their cases and share clinical knowledge. Etai K. 4:1 doi: 10.pregnancy. Association between cigarette 2 smoking and inflammatory markers for cardiovascular disease.   5. Acheson R M. Milewich T. The influence of tobacco smoke on homocysteine level in plasma of healthy males.  19. Psaty B M. Bhagwat V R. 19: 471-474. Fardellone C. Hinc S.reactive protein with cardiovascular disease risk factors and subclinical disease in healthy elderly subjects.jbiomeds. N Engl J Med 1994. 21: 106-110.Dubiel J S.  14. Sztefko K. Stratul S I.ning. Med Hypotheses 2000. ✓ From time to time invited articles. Wardas W. 336: 973-979. Krzysiek J. A pilot study to examine the effects of smoking cessation on serum markers of inflammation in women at risk for cardiovascular disease. Galie N. 65: 486-490. Reichert V. 8: 390-403.cholesterol concentrations seem to be linked with aortic valve stenosis and its progression. Kohn N. 55: 289-293. Tiwari S. and the risk of cardiovascular disease in apparently health man. Total plasma homocyteine and cardiovascular risk profile. Nygard O. aspirin. 50: 285-290. Inflammation.imedpub. Przegl Lek 2007.   2. Goya W. Nakamura M. Cowan J L. Fowkes F G R. Prev Med 2005.   6. 20: 344-353. McGill HC.   9. Kato K. Hyperhomocysteinemia in active and passive smokers and the levels of folate and vitamin B6 in plasma. A quantitative assessment of 2 plasma homocysteine as risk factor for vascular disease. 2   2. Szołtysek-Bołdys I. 136: 212-214. as well as decrease in plasma HDL. Effects of short term cigratte smoking on insulin resistance and lipid profile in asymptamatic adults. Tracy R P. Relationship of plasma homocysteine with lipid profile parameters in ischemic heart disease. European Heart Journal 1999. Hon J. Indian Journal of clinical Biochemistry 2006. Potential mechanisms for the augmentation of atherosclerosis and atherosclerotic disease by cigarette smoking.com 4 © Copyright iMedPub . Tamura T.  25. Sobczak A. Schrier R W. Tonstad S. Jacobsen D. chronic inflammation and ageing may reflect acute-phase induction of pyridoxal phospahate activity. 64: 679-684. McCarty M F. Price J F. JAMA 1990.   3. Clin Chem 1972. Pagar K. 26: 1765-1773. Topping D L . Przegl Lek 2009.  16.com ✓ JBS publishes peer reviewed articles of contemporary research in the broad eld of biomedical sciences.  18. C-reactive protein as a predictor of disease in smokers and former smokers: a review.   7. increase in plasma lipoprotein(a) and triglycerides. Gupta V. Prev Med. Am J Cardiol 2008. Rev port cardiol 2000. Pneumologia 2009.   4. The human costs of tobacco use.  11. systematic reviews. Clin Chem Lab Med 2004. Xue X. Agarwal C G. 63: 16341641. Relation of C-reactive 2 protein to extent and severity of coronary narrowing in patients with stable angina pectoris or abnormal exercise tests. Int J Clin Pract 2009. Bartecci C E.   3.   0. Cushman M. Reis H P. The influence of smoking on plasma homocysteine and cysteine levels in passive and active smokers. Molecular biology and Genetics. meta-analysis. Sobczak A. 2   1. Clin chim Acta 2001. Fredrickson D S. Refsum H. Biochemistry of cellular regulation. Przegl Lek 2008. 41: 651-656. Marszall M L. Publish with iMedPub Follow us: http://www. Beresford S A. Relationship between smoking and cardiovascular risk factors in the development of peripheral arterial disease. Medicalia. 165-183. 24: 3312-3318. Smoking. 330: 907-912.com/ References    1.   8. editorials and review of selected topics will be published. Yadav A S.  15. Vol 2. 86: 205-207.Omenn G S. Fram O B. Levy R I. evidencebased articles. Jr.  17. Influence of smoking on homocysteinemia at baseline and after methionine load. The role of smoking in changing essential parameters in body homeostasis.   4. 42: 408-414. Increased homocysteine associated with smoking. Talwar A. 58: 89-94. Czarnowski W.3823/1009 Our Site: http://www. 1979. Okayama A. Klos M. 274: 1049-1057. You can also access lots of medical publications for free. 1 No. Scope of this journal includes: Biochemistry. Br Med J 1961. Ohsawa M. Effects of smoking on serum concentrations of total homocysteine and B vitamins in mid. ✓ The editorial board of JBS shall strive to maintain highest standards of quality and ethics in its publication. Estimation of the concentration of low-density lipoprotein cholesterol in plasma. 18: 499-502. Arterioscler thromb vasc Biol 1997. Friedewald W T. Secondary research including narrative reviews. Greabu M. Aoun G. Waters D D. JAMA 1995. Rathod I M. Azar R R. Coble Y O. Onoda T.com/ Submit your manuscript here: http://www. Tobacco smoking and serum lipids in old men. Azinheira J. Biomedical sciences. Lifetime smoking exposure affects the 2 association of C. 274: 1526-1533. The Hordal and homocysteine study JAMA 1995. 66: 159-165. Totan A. Reis R P. Indian J Physiol Pharmacol 2006. Mowbray P I. Makarowski R. 28: 1108-1111.Vollset S E. The worldwide smoking epidemic: council reports. Folan P.  13.

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