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GRAM NEGATIVE BACILLI Enterobacteriaceae Citrobacter Edwardsiella Enterobacter Aeromonas Escherichia Klebsiella Morganella Proteus Providencia Salmonella Serratia

Burkholderia Shigella Yersinia Pseudomonas Vibrio Stenotrophomonas Bartonella Campylobacter Helicobacter Legionella Brucella Bordetella Francisella Streptobacillus Pasteurella (except aerogenes=cb)

GRAM NEGATIVE COCCI Neisseria Moraxella

NO GROWTH ON BLOOD AGAR Neisseria gonorrhoeae Haemophilus influenzae

GRAM NEGATIVE COCCOBACILLUS Pasteurella aerogenes Haemophilus GRAM POSITIVE BACILLI Listeria Corynebacterium Gardnerella (gram variable) Lactobacillus GRAM POSITIVE COCCI Staphylococcus Micrococcus Stomatococcus Streptococcus Viridans streptococci Enterococci GROWTH ON HE (Hektoen enteric) Citrobacter—colorless/yellow w/blk centers Edwardsiella—colorless with black centers Proteus—colorless with black centers Salmonella—colorless with black centers Enterobacter—yellow Escherichia coli—yellow Hafnia—colorless Klebsiella—yellow Morganella—colorless Providencia—colorless Serratia—colorless Shigella—colorless Yersinia—Salmon (if it ferments lactose)

GROWTH ON MacCONKEYS Pseudomonas—NLF Burkholderia—NLF Yersinia—NLF/colorless to peach Stenotrophomonas—NLF Edwardsiella—NLF Hafnia—NLF Morganella—NLF Proteus—NLF, may swarm Providencia—NLF Salmonella—NLF Shigella—NLF Serratia—late LF/may be red Aeromonas—NLF & LF Citrobacter—NLF & late LF, light pink Vibrio—NLF some LF Klebsiella—LF mucoid Enterobacter—LF Escherichia coli—LF pink

OXIDASE POSITIVE ORGANISMS Neisseria Moraxella Pseudomonas Vibrio alcaligenes Burkholderia Pasteurella Campylobacter Haemophilus (variable) Bacillus (only gram pos) Helicobacter Brucella CATALASE POSITIVE Staphylococcus Neisseria Enterobacteriaceae (all) Haemophilus Listeria Francisella

GLUCOSE ONLY FERMENTERS Yersinia Escherichia (variable) Salmonella Shigella H2S PRODUCTION Proteus Edwardsiella Citrobacter Salmonella INDOLE POSITIVE Citrobacter (some) Escherichia coli Klebsiella oxytoca METHYL RED POSITIVE Citrobacter (some) Escherichia coli Klebsiella oxytoca PYR POSITIVE Enterococci Streptococcus pyogenes UREASE POSITIVE Providencia Morganella Proteus—really fast Klebsiella—Yersinia—Enterobacter—slow X+V FACTOR DEPENDENT Haemophilus influenzae Haemophilus aegyptius Haemophilus haemolyticus X FACTOR DEPENDENT Haemophilus ducreyi Haemophilus aphrophilus V FACTOR DEPENDENT Haemophilus segnis Haemophilus parahaemolyticus Heamophilus parainfluenzae Haemophilus paraphrophilus CAMP TEST POSITIVE Streptococcus agalactiae Listeria LACTOSE FERMENTERS/COLIFORMS Escherichia Citrobacter Enterobacter Klebsiella CITRATE POSITIVE Citrobacter Enterobacter Enterobacter VOGES-PROSKAUER POSITIVE Enterobacter Klebsiella ANAEROBIC GNR NONSPOREFORMING Bacteroides Prevotella Porphyromonas Fusobacterium Bilophilia ANAEROBIC GPR NONSPOREFORMING *Lactobacillus *Actinomyces Bifodobacterium Propionibacterium Eubacterium Mobiluncus ANAEROBIC GPR SPOREFORMING Clostridia perfringens Clostridia tetani Clostridia botulinum Clostridia difficile AEROBIC GPR SPOREFORMING Bacillus subtilis Bacillus anthracis Bacillus cereus AEROBIC GPR NONSPOREFORMING Mycobacteria Listeria Corynibacterium .