This action might not be possible to undo. Are you sure you want to continue?
Biochemistry Cumulative Exam
Consider this one a "freebie." The 50 questions on this exam came straight out of Zubay's Biochemistry textbook. Any self-respecting biochemist should be able to answer them (with some thought). Therefore, at least 75% of the questions must be answered correctly to pass this exam. No books, notes, or references of any kind may be used on this exam; it has been designed to test what you know. (If you do not pass it, then at least you will know what areas you need to review.) Good luck, K. Redding
# answered correctly: # answered incorrectly: Grade:
_________ _________ _________
D. negligible positive negative dependent on ∆ PV is ∆E 6. 7. this reaction will occur spontaneously. this reaction is so strongly favorable that it could be coupled to drive an otherwise unfavorable cell reaction. endoplasmic reticulum.1. 5. 3. the total entropy of the the total entropy of the in any reaction that occurs spontaneously in any reaction that occurs spontaneously universe remains constant. B. by an internal network of filaments called A. In most biochemical reactions. D. C. Golgi apparatus. E. depending on the actual concentrations of reactants and products. Thus. Liquid and solid water have the same number of hydrogen bonds per molecule of water Liquid water has more hydrogen bonds per molecule than does solid water Solid water has more hydrogen bonds per molecule than does liquid water Hydrogen bonding is a major factor in the structure of gaseous water None is true Page 1 . According to the Second Law of Thermodymnamics. the three-dimensional integrity of the cell is maintained. in part.2 kcal/mole. B. C. D. ribosomes. mitochondria. D. cytoskeleton. In plant and animal cells. C. C. chloroplasts. the the the the cell wall. Of the following. the total entropy change must be greater than 0. C. universe is always decreasing. the nucleus. B. B. C. D. this reaction will only occur spontaneously if it is coupled to another reaction that has a more favorable G°. Which of the following is true? A. this reaction may or may not occur spontaneously. A. D. viruses yeast B. C. G° for the conversion of glycerol-3-phosphate to glycerol + Pi is -2. Each of the following subcellular structures contains DNA EXCEPT A. the total entropy change must be less than 0. 2. B. A. bacteria plant cells 4. you can conclude that A. which are prokaryotes? A. B. D.
B. E. B.47 4.45 x 10-5 M? A. Cys. B. C. In parallel and antiparallel sheets consecutive R-groups on each peptide strand alternate sides of the sheet. D. and a tripeptide.53 9. B. a and b E.4-dinitrofluorobenzene urea 2-mercaptoethanol phenylisothiocyanate cyanogen bromide Page 2 . C. Because of the free rotation around the alpha carbon.54 none of the above 9. 12. A reagent commonly employed to cleave disulfide bonds in proteins is A. Chymotrypsin treatment of the peptide produced a dipeptide that contained sulfur and has a UV absorbance. Phe. Lys. D. Which is true regarding the orientation of the R-groups in beta structures? A. Val-Ala-Lys-Phe-Cys Cys-Lys-Phe-Ala-Val Cys-Ala-Lys-Phe-Val Cys-Phe-Lys-Ala-Val Val-Phe-Lys-Ala-Cys 10. E. C. C. Exposure of the peptide to trypsin produced a dipeptide and a tripeptide. the R-groups can seek the least crowded region and are not restricted to a specific side of the sheet. A peptide was found to have a molecular mass of about 650 and upon hydrolysis produced Ala. and Val in a 1:1:1:1:1 ratio. Deduce the sequence of the peptide. B. E. D. C. Which amino acid has an R-group pKa closest to physiological pH? A.47 5. 3. In the parallel beta sheet structures the amino acid R-groups are all on the same side of the sheet. What is the pH of an aqueous solution that has a [OH-] of 3. D.8. In the antiparallel beta sheet structure the R-groups on alternate strands are on the same side of the sheet. D. 2. E. A. lysine histidine glutamate arginine aspartate 11. The peptide upon treatment with Sanger's reagent produced DNP-Cys and exposure to carboxypeptidase produced valine.
Hemoglobin is an allosteric protein. urease urease. Hemoglobin has quaternary structure. B.500).9. hemoglobin. 482. catalase. all of the substrate to be converted to product. gives half of the maximum reaction rate. 15. wt. hemoglobin hemoglobin.700). D. catalase (pI = 5. Which of the following would allow you to determine the isoelectric point of a protein? A. 37.500) were applied in a pH 6. wt. The Km is: A. C. 64. Hemoglobin is composed. catalase. urease cannot be determined from the information given 17. lactoglobin. E. With respect to a relaxed muscle. What was their order of elution? A. C. C. protein protein protein protein protein quaternary structure solubility as a function of pH size shape tertiary structure 16.100) and hemoglobin (pI 6. Hemoglobin is an oxygen transport protein. in part. is produced when the enzyme is saturated with the Page 3 . B. The time for The time for The [S] that The [S] that The [P] that substrate. E. urease. C. wt. D. B. Copper ions are important in hemoglobin's ability to bind oxygen. B. catalase. E. Which of the following does not describe hemoglobin? A. E.2. wt. mol.6. gives the maximum reaction rate.1. C. B. hemoglobin. mol. which of the following is true? A. D. D. mol. 247. lactoglobin. mol. A mixture of urease (pI = 5. lactoglobin (pI 5. D. E. of an iron containing heme group. the number of actin-myosin is shortened the number of actin-myosin is stretched the number of actin-myosin is shortened the number of actin-myosin is stretched none of the above cross-bridges is maximized and the muscle cross bridges is maximized and the muscle cross bridges is minimized and the muscle cross bridges is minimized and the muscle 14. half of the substrate to be converted to product.13. lactoglobin lactoglobin.5 buffer to a DEAE-cellulose chromatography column and eluted with the same buffer. catalase.
B. D. Phosphorylation. D. Co2+ D.18. which of the following processes is not an important method for regulating the activity of enzymes? A. C. Mn2+ E. D. Fe3+ B. Under physiological conditions. D. C. An holoenzyme is A. This is because the GDP that is produced is rephosphorylated to GTP at the expense of ATP by which of the following enzymes? A. C. adenylate kinase ATPase GTPase nucleoside diphosphate kinase phosphorylase a Page 4 . The use of nucleotides such as GTP in a metabolic reaction is energetically equivalent to the use of ATP. The conversion of glucose to maltose A. E. C. Adenyl addition. Partial proteolysis. Zn2+ 20. Which of the following will probably not affect the rate at which an intermediate in a metabolic pathway is utilized by the appropriate enzyme? A. Mg2+ C. an equilibrium a hydrolysis reaction an dehydration process an epimerization a mirror image 23. Temperature changes. B. E. E. Some non-serine proteases contain A. C. E. 21. E. formation of a multienzyme complex involving the enzymes of the pathway increasing availability of the substrate for the first enzyme in the pathway a decrease in the concentration of the product of the last enzyme in the pathway addition of water to the organism's growth medium increase the temperature from 30° C to 37° C 22. Disulfide reduction. a coenzyme an enzyme with its cofactor an enzyme lacking its cofactor an allosteric enzyme a cofactor 19. B. B. D. B.
C. NADP+ C. D. E. ATP E. C. B. Electrons from complex I are transferred directly to which of the following electron transport components? A. C. D. C. Which of the following is the hydrophobic electron carrier which is mobile within the membrane? A. D. The electrons transferred in the light reactions of photosynthesis end up in which molecule? A. fluorescence resonance energy transfer donation of the excited electron return to the lower energy level with loss of energy as heat 29. which is the desired pathway for de-excitation? A. E. B. ubiquinone complex II cytochrome a cytochrome b cytochrome c 27. NAD+ B. citrate fumarate oxaloacetate pyruvate succinate 25. E. If an antenna chlorophyll absorbs energy from a photon.24. E. The ultimate acceptor of the electrons removed by oxidative decarboxylation in the TCA cycle is A. D. B. B. B. O2 26. Which of the following intermediates is a substrate for the only membrane-associated enzymatic activity in the TCA cycle? A. complex Ia complex II complex III cytochrome c ubiquinone 28. FAD D. NADPH FADH2 water oxygen carbon monoxide Page 5 . C. D.
C. E.4) β(1. the cells will preferentially transport and utilize the D-glucose until its concentration is depleted and then will start to import and catabolize the lactose. C. C. The conversion of acetoacetate to acetone A. This phenomenon is called "diauxie. E. Which of the following is true concerning the peptide portion of peptidoglycans? A. might be non-enzymatic is facilitated by a resonance stabilized carbanion changes one ketone body into another occurs in the mitochondria all of the above 35. D.4) α(1." What is a likely reason for this phenomenon? A. B. coli are placed in a solution with both D-glucose and lactose. E. C.6) heteropolymer heteropolymer homopolymer homopolymer homopolymer 32. B. B. D. contain contain contain contain c and d only amide bonds analogous to those in proteins ester linkages amide bonds involving the epsilon amino group of lysine amide bonds involving the gamma carboxyl group of glutamate 31. C. α(1. The biological role of cholesterol is which of the following? A. B.4) β(1. Which of the following describes the most abundant energy storage polysaccharide in nature? A. D.4) β(1. to to to to to help reseal arteries which have developed leaks make membranes more fluid transfer light to P700 in photosynthesis transfer electrons to chytochrome c cause membranes to fluoresce 33.30. D-glucose is a competitive inhibitor of the lactose transport system the lactose transport system is not produced until the glucose is consumed carbohydrates cannot be transported until glucose catabolism produces ATP in the cells the cell membrane contains an antiport system which pumps lactose out while transporting D-glucose into the cell 34. D. D. adipose tissue intestine liver pancreas skin Page 6 . Dietary fats (triglycerides) are imported into the body by which tissues? A. D. E. When cells of E. C. B. E. B.
Lysine Serine Histidine Aspartate None of the above 41. coli the last common intermediate for the synthesis of phosphatidylethanolamine and phosphatidylglygerol is A. B. HMG-CoA is synthesized A. C. diacylglycerol phosphatidylserine B. the pathway is connected to another pathway responsible for the synthesis of A. B. C. B. Which of the following amino acids does not play an important role in the active site of serine proteases? A. oxidation of cystine residues. During the formation of glycocholate. B. E. CoASH. The The The The The reduction of cysteine residues. CDP-diacylglycerol phosphatidic acid 37. C. In animals. What reaction intermediate would you expect in this process? A. and ATP form cholyl-CoA. in on in on on the the the the the cytosol and mitochondrial matrix endoplasmic reticulum and plasma membrane Golgi apparatus primarily endoplasmic reticulum and outer mitochondrial membrane plasma membrane 39. D. In E. pyrimidine nucleotides coenzyme A flavonoids Purine nucleotides heme 40. E.36. AMP. D. Page 7 . E. cholic acid. E. The protein thioredoxin is involved in which of the following processes? A. Although only one amino acid is synthesized by the histidine pathway. B. reduction of cystine residues. D. C. D. deamination of cystine residues. C. D. C. and pyrophosphate. cholyl cholyl cholyl cholyl phosphate adenylate pyrophosphate group bound to a protein by a thioester linkage 38. D. phosphorylation of cysteine residues.
a graph of velocity versus substrate concentration for enzyme G will have the shape of a parabola. which are commonly first-order.3-bisphosphate to glycerate-3-phosphate glyceraldehyde-3-phosphate to dihydroxyacetone phosphate 47. If enzyme "G" is first-order and enzyme "N" is fourthorder. Which of the following conversions is catalyzed by the enzyme 3-phosphoglycerate kinase? A. Lipid components of membranes do not readily move from one side of a bilayer to the other. D. 5 46. Regulated enzymes frequently exhibit a higher-order response to substrate concentration than non-regulated enzymes. glycerate-2-phosphate to phosphoenol pyruvate glycerate-3-phosphate to glycerate-2-phosphate glycerate-1. false 48. C. C. A. an electron acceptor. a water soluble substrate. A β-D-aldopentofuranose has how many chiral carbons? A. B. E. enzyme G will require energy from ATP to produce a product. B. 45. enzyme N will be more sensitive to changes in substrate concentration than enzyme G. 44. true B. C. B. magnesium copper cobalt chromium zinc 43. but not enzyme N. D. E. B. 3 D. D. D. a hydride ion acceptor.42. Phosphatidylinositol A. enzyme G is said to be cooperative. It It It It It is is is is is an oxidizing agent. Vitamin B12 contains which of the following metals? A. 4 E. a reducing agent. B. only functions as an intermediate in the biosynthesis of of phosphatidylinositol-4 phosphate in eukaryotes does not occur in the membranes of eukaryotes constitutes about 5 % of the phospholipids in prokaryotes may be converted to two phosphatidylinositol derivatives that yield cellular second messengers in animal cells Page 8 . A. 0 B. Which of the following processes does not describe the action of NAD+? A. 2 C. D. C. C.
guanine. consists of A. How many molecules of δ-aminolevulinate are required to make one molecule of porphyrin? A. ribose. B. D. and three phosphoryl groups GMP plus two phosphoryl groups guanine. D. and three phosphoryl groups Page 9 . B. dGTP. The nucleotide. C. 2-deoxyribose. E. and three phosphoryl groups guanosine.49. 2 4 6 8 none of these 50. C. 2-deoxyribose.
7/16/99 No. on Q-Bank Test Correct Answer 1 1 1 2 2 2 3 3 4 4 5 5 6 6 7 7 8 8 9 10 12 13 14 15 15 16 16 17 17 18 18 19 20 21 21 22 23 23 24 9 10 11 11 12 13 14 19 22 25 26 29 3 4 29 3 4 24 2 21 2 21 2 22 2 20 2 26 2 24 16 11 17 18 14 17 15 19 14 18 33 4 4 3 34 4 4 27 3 25 6 12 16 11 8 10 8 28 11 24 6 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 B C B B A A C D D B C C D D B B C B E B D C D E E A E B A E C B B E B B B A D A D C C A D C A D D C Page 1 . in No.Answer Sheet for Test “Cume 7/99”.
This action might not be possible to undo. Are you sure you want to continue?