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Sharif Matouq Ahmed Hala Tel: 02-6610061 Saudi National ID: 1001654456 Mobile: 0501057777 E-mail: Sharif.Hala@gmail.


Career Objective
- To work in a challenging and a professional medical research position which offers interesting prospects of growth, progress and advancements.

- To utilize my time management, problem solving, creative thinking and versatile lab skills along with my determination and the ability to work independently in a modern research environment. - To utilize my interpersonal and distinguished communication skills, international networks in contributing to cutting edge research and international collaborations to study complex genetic traits in relation to disease. - To use my background in molecular biology, medical genetics, stem cell and musculoskeletal science to develop new methods and protocols. - To excel in the fields of biomedical research and to significantly contribute towards the growth of a dynamic organization, which offers challenging opportunities for proficient accomplishments.

• University of Oxford, UK – MSc - By Research April2010 - Jan2013 Masters degree in Science by research in Stem Cell Epigenetics & Musculoskeletal Sciences. • University of Aberdeen, UK - MSc Aug2008 - Jan2010

Masters degree in Medical Molecular Genetics: a one-year degree, which includes a six months research project. • University of Aberdeen, UK - BSc May2003 - Jun2008

Bachelor degree in Molecular Biology with Honors. : A four-year degree and a one-year honor research/work year. • University of Wisconsin Superior, Wisconsin, USA - TOFEL intensive course at SESLI office for one year. - Introduction to Biology and Environmental science: a one-year course was directed toward understanding environmental issues and organismal behavioral due to cultural changes. Oct2000 - Jul2002

Research Experience
• More than 40 months of hands-on lab research experience as follows: M Sc by Research (Jan2010-Nov2012) • The use of femtosecond near infrared laser microscopy for targeted transfection of different somatic mammalian cells as well as adipose derived stem cells with non-viral vectors containing transcription factors (Oct4, Sox2 and Klf4) individually into induced pluripotent stem cell generation. • Generation of the induced pluripotent stem cell using non-viral methods (Originally generated by S. Yamanka the NOBEL prize winner 2012 on the generation of HiPSCs) by electroporation of three plasmids containing embryonic reprogramming factors (Oct4, Sox2 and Klf4) and detection plasmid as green fluorescent protein. • Differentiation of human induced pluripotent stem cells into mesodermal linage into chondrocyte to form cartilage for future clinical trials. The project aimed to employ this novel embryonic stem cell-like resource to promote chondrogenic differentiation using a new pluripotent and possibly autologous source. M Sc Project (Feb 2009-Jan2010) Institute of M edical Sciences, University of Aberdeen • A genome-wide scan (GWAS) of 3500 osteoporotic females investigation: An association study of NADSYN1 gene was investigated using different single nucleotide polymorphisms to determine the effect of vitamin D serum levels and different mutations within the NADSYN1. H onor degree research placement (August 2007- June 2008) Institute of M edical Sciences, University of Aberdeen • The effect of drug mixtures against Candida species such as: Caspofungin. Drugs concentrations were compared against growth, cell number and other parameters to determine the effect on fungal species.

- Abstract accepted by the British Society for Matrix Biology (BSMB): (2011) Directed Differentiation of H uman Induced Pluripotent Stem Cells into Chondrocytes S Hala¹, C Yapp1, A Kramm1, L Carpenter2, S Watt2, A Carr1, J Dunford1, U Oppermann1, U Tirlapur1 - Poster and oral presentation at the Annual Symposium of Stem Cell – Oxford 2011 H uman Induced Pluripotent Stem Cells differentiation into Cartilage under H ypoxia S Hala¹, C Yapp1, A Kramm1, L Carpenter2, S Watt2, A Carr1, J Dunford1, U Oppermann1, U Tirlapur1 - Effect of Hypoxia on the Transcriptional Profile of hESCs-Derived Cortical Precursors ¹El-Sherbini YM, Hala SH, George J, H Ye, ¹ZF Cui (in preparation for submission to J Molecular Neurology) - Comparison between human induced pluripotent stem cells and embryonic stem cells differentiation into motor neurons under different conditions ¹El-Sherbini YM, Hala SH, George J, H Ye, ¹ZF Cui (Manuscript is in the write phase)

- Invited Speaker at the Dunn School of Pathology for research on Chondrogenic differentiation of Human Induced Pluripotent Stem Cells (2012) July - Several presentations at the Botnar Research Centre, Institute of Musculoskeletal Science, Oxford University including the Annual research development and discussion (2010-2012) - Single nucleotide polymorphisms to determine the effect of vitamin D serum levels and different mutations within the NADSYN1, University of Aberdeen (August 2009) The Annual Postgraduate Presentation Projects - Bone Society for Matrix Biology Annual meeting, University of Bristol (Jul 2011) – Presented poster and accepted abstract

• Statistical analysis of culture and genetic studies including: - T-test and pedigrees formation family studies and bioinformatics - Able to utilize statistical programs such as Microsoft Excel and Prizm for data blotting and analysis • Several Lab Skills including: - Culture of prokaryotic and eukaryotic cells including stem cells, reprogramming somatic cells into induced pluripotent stem cells (embryonic stem cell-like) and differentiation - Electroporation & opto-poration transfection of DNA into tissue/cells - Advanced molecular biology techniques such as protein purification, identification and quantification as well as cloning in bacteria and yeasts along with wide range of DNA analysis and genotyping protocols. • Advanced equipm ent used include: - Advanced confocal/multiphoton microscope: such as Bicton Dickson Pathway, Olympus BX40 and Zeiss LSM710 - Real time quantitative PCR systems such as: ViiA 7TM Real-Time PCR system - Fluorescent readers such as : FLUOstar Optima - Spectrophotometers such as: Spectra MAX - Fluorescent activated cell sorting. (FACS) Protein purification such as: Chromatography and HPLC DNA sequencers such as Ion Personal Genome Machine® (PGM™) Sequencer Hypoxic chambers Electroporation equipment such as the Neon® system

• Expert user of Microsoft Office, endnote, refworks, iWORK as well several MAC/Linux system programs • Language: Arabic (Mother tongue) and English (fluent in listening, speaking and writing)

Seminars and Lectures
Attended several seminars, lectures and work groups in several places on biomedicine, anatomy, genetic diseases, and several other genetic and biochemical research findings.

- High School Student of the Year (1998) - Prize for excellence of research from United Kingdom Cultural Buru (2011) - One of the 49 Saudis students honored by the Saudi Ambassador Prince Muhammed Bin Nawaf AlAbdulaziz for excellence in British and Irish universities (2012)

In addition to the attached ones, further references are available upon request.