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TA

TA

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Published by Nofrianto

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Published by: Nofrianto on Feb 14, 2013
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07/30/2014

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bilas tube sebeleumnya dg 0.5 – 1 ml acetone Masukan sampel ke tabung sentrifuge bilas mortar dg acetone dingin dan masukan bilasan ke tabung centrifuge + 2 ml hexane kocok centrifuge shg emulsi pecah + 2ml air Pigment layer + hexane Air + acetone + Na2SO4 anhidrat tutup rapat dan diamkan 5’ Pindahkan ke tube lain.Sampel 0.5 ml hexane.5 – 1g daun bayam yang dicincang Masukan sampel ke mortar + 2 ml acetone giling sampai halus Jika banyak acetone yg menguap + 0. Column chromatography KLT Spectro UV / Vis .

Sampel ( 20 g mesocarp buah palm ) Sampel dibungkus dg kertas saring dan dimasukan ke reflux flask 500 ml + 300ml hexane Extraction ( soxhlet) T 60 C Selama 6 jam sampai warna sampel memudar o saring Saring dg kertas saring Whatman No.00001 g sampel dlm 25 ml Iso octane Penentuan β . 1 Larutkan 0.carotene Ukur absorban dg spectro 25 UV / Vis dg lambda 446 .

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