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Common Stains and Their Reactions

Common Stains and Their Reactions

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Published by Godwin Halfon

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Published by: Godwin Halfon on Mar 12, 2013
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Common Stains and Their Reactions

Stain Haematoxylin Eosin IronHaematoxylin Van Gieson Verhoeff's Elastin Tartrazine Silver Impregnation Methyl Green Nuclear Fast Red Gomori's Trichrome Heidenhain's Azan Osmium tetroxide Alcian Blue Periodic acidSchiff's reagent (PAS) PTAH Masson's Trichrome Luxol Fast Blue Aldehyde Fuchsin Light Green Gallocyanin Romanowsky(e.g. Leishman's stain) Aldehyde Pararosanilin Eosin ▪ Stains cytoplasm pink to red; red blood cells are also bright red. ▪ Common counterstain to hematoxylin. ▪ Stain intensity varies with the formula as well as the fixative. Hematoxylin ▪ Stains nuclei blue to dark-blue. ▪ Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue. ▪ Stains myelin weakly but is not noticeable if combined with eosin stain. Periodic acid-Schiff (PAS) ▪ Stains glycogen, mucin, fungus, basement membrane and other substances. ▪ Stain used to detect fungal organisms and cytoplasmic accumulation of glycogen. ▪ Stains lysosomes granules red-purple, can be used in recognition of macrophages. Alcian Blue ▪ Stains mucopolysaccharides or glycosaminoglycans ▪ cationic dye (positively charged molecule) for the demonstration of glycosaminoglycans. Nucleus blue blue/black black dark green red purple/red red blue blue/black dark blue blue Cytoplasm pink brown/ yellow yellow light green pink purple purple/red red pink Collagen pale pink red yellow grey/brown light green pink green deep blue brown pink red green/blue light green RBCs bright red yellow yellow green pink red red brown blue red variable nucleic acids, Nissl granules - dark blue acidophils - redbasophils blueazurophilic - purple elastic fibres - purple Other mucins - light blue colloid - pink muscle - red muscle: yellow/browncartilage pink elastic fibres - black reticular fibres - black

keratin - redmuscle purple/red muscle - red myelin, lipids - black mucins, - blue mucins, glycogen, glycocalyx - magenta muscle bands - blue cartilage, mucins - blue or green; muscle - red myelin - blue~ elastic fibres, mast cells deep purple

PhosphoTungstic Acid Hematoxylin (PTAH) ▪ Stains nucleus and cytoplasm detail and connective tissue fibers. and analine blue) are utilized. ▪ A synthetic dye in the thiazins family.▪ binds anionic (negative) sites on the polysaccharide. ▪ Historic stain used to show CNS reactive astrocytes now used immunochemistry for glial fibrillary acidic protein (GFAP). Stains brain and spinal cord parenchymal tissue dusky pink to red. ▪ This is a combination of Verhoeff’s elastic stain which is a hematoxylin stain containing ferric chloride and Wright’s iodine solution and Van Gieson stain which contains acid fuchsin. ▪ Although called a trichrome. Toulidine Blue ▪ Stains nucleus blue and cytoplasm light blue. Verhoeff-Van Gieson ▪ Verhoeff-Van Gieson or elastic-Van Gieson (EVG) stain. skeletal and smooth muscles red. picric acid. and nuclei blue to black. and hematoxylin. acid fuchsin. . four dyes (hematoxylin. collagen and mucin blue. collagen pale red. Masson’s Trichrome Stain ▪ ▪ ▪ ▪ Stains nuclei deep blue. other tissue elements yellow. and striated muscle blue. fibrin blue. ▪ can be combined with H&E and VG staining methods. ▪ Stains elastic fibers blue-black to black. Biebrich scarlet. Used to evaluate fibrosis Striations in skeletal muscles also shows up much better in Masson’s trichrome than in hematoxylin and eosin stain. ▪ Stains collagen pink.

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