This action might not be possible to undo. Are you sure you want to continue?
Description: Human Insulin ELISA Qty/Pk: 96-well strip plate Background Information: Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas. Key Applications: ELISA Application Notes: 2 hour assay, room temperature Sample ≤ 20 µL serum, plasma, or tissue culture medium Species Reactivity: Human
Entrez Gene Summary: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. UniProt Summary: FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. SIZE: 110 amino acids; 11981 Da SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds. SUBCELLULAR LOCATION: Secreted. DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730]. SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family. Linearity of Dilution: 96–112% Precision, %:
Inter-assay: 9.1–11.4 Intra-assay: 4.6–7.0
Accuracy: 76–103% Detection Methods:
• • •
INS proinsulin insulin
Standard Curve Range: 2–200 µU/mL