PENGARUH FRAKSI ETANOLIK HERBA PEGAGAN

(Centella asiatica Urb.) TERHADAP PENINGKATAN TITER ANTIBODI
PADA MENCIT JANTAN GALUR BALB/C YANG DIINDUKSI VAKSIN
HEPATITIS B

Usulan penelitian untuk skripsi

Diajukan oleh

Pramita Yuli Pratiwi
7124/FA

Kepada

FAKULTAS FARMASI
UNIVERSITAS GADJAH MADA
YOGYAKARTA
Maret 2008

.............................................................. 8 B.......... Tujuan Penelitian .......................................................................... Perumusan Masalah ........... 6 A................................................... Kegunaan Pegagan ........... v JUDUL................................................................................................ 2 A............................ Morfologi Tumbuhan .......... 13 ...................................................................................................... 5 TINJAUAN PUSTAKA ................................................................................. 4 C............................................. Pentingnya Penelitian diusulkan ........................................ iii DAFTAR GAMBAR ........................................ Sistematika Pegagan......... 11 E.... i HALAMAN PERSETUJUAN....DAFTAR ISI Halaman HALAMAN JUDUL ................................................................................. ...................................................... 6 1.................................................................................................................................. 2 B...... Latar Belakang Masalah ................................) ................................................... 9 D............... 7 4............................................................. 6 3........... Penyarian ...... ii DAFTAR ISI .. 4 D........................................................................ 6 2......................................... Kandungan Pegagan ................................................................................................................................................................................................ 1 PENDAHULUAN ....................... 1 INTISARI .................. Hepatitis B...................... Engerix B®......................... Pegagan (Centella asiatica Urb........................................................ Vaksinasi ..................................................... 8 C.............

.......................... 24 DAFTAR PUSTAKA......... Pemberian vaksin hepatitis B ....................................... 20 4............... 22 C........................................................... 23 JADUAL PENELITIAN .................... Rencana Penelitian ....... Pengkondisian hewan uji dengan larutan uji ........................................................................... 20 5... Determinasi tanaman pegagan ................. 20 6................................. 17 I.. ELISA (Enzym Linked Immunosorbant Assay).. ........................................................................................................................................... 19 2............ 15 H............................................................................F................................................. 20 7............... Antigen............... 25 ................ Pengukuran titer antibodi dengan metode ELISA ............... 21 B........................ 22 FASILITAS YANG DIPERLUKAN ................................................ Analisis data.................. Pengumpulan serum hewan uji ... 19 A.............. Definisi Variabel Operasional................................ 14 G....................................................................................................................................... 17 KETERANGAN EMPIRIK.......................................................................................................... Pembuatan fraksi etanolik herba pegagan ............. 19 3............................................................................. Sistem Imun......................... Sampel dan Teknik Sampling.............. Antibodi... 18 RENCANA PENELITIAN .... 19 1...................................

...DAFTAR GAMBAR Halaman Gambar 1.......... 15 ............................... 12 Gambar 2.................. Sistem imun ................................ Vaksinasi .......................................................................

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