( Lab. no.

8 )
Osmotic fragility
Osmotic fragility
of RBCs
of RBCs
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Types of Solutions:
Hypotonic Sol.
Isotonic Sol.
Hypertonic Sol.
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Principle
Principle
:
:
The responses of RBCs to
solutions of different osmotic
activities are examined after one
hour of adding fresh blood to
these solutions.
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Procedure
Procedure
:
:
CAdd (1–2) drops of fresh blood to solutions of NaCl
with concen.:(0.1%, 0.3%, 0.45%, 0.7%, 0.9%, and 10%).
CAdd (1–2) drops of fresh blood to solutions of Glucose
(1%, 5%, and 10%).
CAfter one hour examine the response of RBCs to the
different solutions of NaCl and Glucose.
CMicroscopic examinations may be performed.
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NaCl
Glucose
0.1% 0.3% 0.5% 0.7% 0.9% 10%

1% 5% 10%
NaCl
Glucose
0.1% 0.3% 0.5% 0.7% 0.9% 10%

1% 5% 10%
After ONE hour
12
3 9
6
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0.1-0.33% NaCl

1.0% Glucose
very hypotonic sol.


O True solution.
O No supernatant.
O Transparent.
O Complete hemolysis.
0.33%-0.48% NaCl
hypotonic solution
O Mixture at the
beginning is turbid.
OSupernatant
(yellowish or
reddish clear and
transparent).
OPartial haemolysis
0.5%-0.7%-0.9%-10% NaCl
5%-10% Glucose
Slightly hypotonic
Isotonic and
Hypertonic solution
O Mixture at the
beginning is turbid.
OSupernatant colorless.
ONo haemolysis
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