Complementation test

:
Spy 13-28 /CyO males were crossed with heterozygous females containing deletions on one of the chromosomes. Resulted straight winged progeny were tested for fertility.

Recombination mapping:
Recombination frequency with exu To perform genetic mapping, 0-1 day old spy 13-28 males were crossed with exu’ /Cyo virgin females. The straight winged virgin females from the F1 progeny was crossed with Cyo/Gla males. All the CyO males were collected and were crossed with E2/ CyO virgin females. The straight winged male progenies were collected from the cross and were checked for fertility. Recombination frequency was calculated. Recombination frequency with BSC404 To perform genetic mapping, 0-1 day old spy 13-28 males were crossed with BSC404 /Cyo virgin females. The straight winged virgin females from the F1 progeny was crossed with Cyo/Gla males. All the CyO males collected and were crossed with E2/ CyO virgin females. The straight winged male progenies were collected from the cross and were checked for fertility. Recombination frequency was calculated.