Transformation Advantages and Disadvantages Advantages: any DNA can be used, if DNA isn't useful can be used for

building material Disadvantages: recipient must be competent, DNA must be similar enough to undergo homologous recombination, transferred DNA must escape DNases in environment

Generalized Transduction

Occurs when a bacteriophage accidentally packages host DNA into the bacteriophage capsid Can then enter other cells via bacteriophage

Generalized Transduction Advantages and Disadvantages

Advantages: any piece of DNA can be acquired, DNA is protected by phage head, recipient does not require a special program Disadvantages: bacteriophages have limited host range, homologous recombination is required

Specialized Transduction

Errors in excision by prophage results in part of the bacterial chromosome being incorporated into the phage Gene is part of the phage genome as long as the phage is still functional

Specialized Transduction Advantages and Disadvantages

Advantages: DNA is protected from DNases, no special program required by recipient, homologous recombination is not required Disadvantages: Gene transfer is limited, bacteriophage must be able to infect both host and recipient, high risk (phage may kill it)

ConjugationAdvantages: DNA is protected from DNases, everything needed is provided by plasmid, homologous recombination is not required, highly promiscuous, Conjugation has advantages over other forms of genetic transfer including minimal disruption of the target's cellular envelope and the ability to transfer relatively large amounts of genetic material Disadvantages: plasmid replication is somewhat host restricted (may not be able to replicate in recipient), gene transfer is limited to those associated with plasmid (can accumulate transposons)

Sign up to vote on this title
UsefulNot useful