P. 1
Act 02 Culture_Media_Preparation.pdf

Act 02 Culture_Media_Preparation.pdf

|Views: 7|Likes:
Published by Bryan Janier
Industrial Microbiology
Industrial Microbiology

More info:

Categories:Types, School Work
Published by: Bryan Janier on Jun 08, 2013
Copyright:Attribution Non-commercial


Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less





Activity No.

2 Culture Media Preparation Culture media are composed of nutrients used for the growth and identification of microorganisms. They may be classified as follows : I. according to the physical state : 1.1 solid media – 1.5% to 2.0% agar is added as a solidifying agent 1.2 semi-solid media – with 0.5% to 1.0% agar 1.3 liquid – no agar is added II. according to use : 2.1 2.2 simple media enriched media for the cultivation of non-fastidious microorganisms. e.g. nutrient agar contain additional nutrient sources for the growth of fastidious microorganisms e.g. blood agar plate, chocolate agar plate, brain heart infusion media contain nutrients that allow a particular organisms that might not be present in sufficient numbers in the specimen to be isolated and identified e.g. Selenite F – for the enrichment of Salmonella typhi in a fecal specimen contain ingredients that inhibits the growth of certain bacteria while permitting the growth of others e.g. mannitol salt agar for the growth of salttolerant staphylococci allow colonies of one kind of organism to be distinguished from another. The media usually have constituents that cause observable changes in them when a particular biochemical reaction occurs. e.g. MacConkey agar allows the differentiation of lactose fermenters (red colonies) from nonlactose fermenters (colorless colonies)


enrichment media



selective media



differential media


Culture media which can not be heated are sterilized through filtration. The most popular among these at present is the membrane filter such as the cellulose nitrate filter.

e. 7. 1. Brock's Microbiology. cooled. E. paper strips or bacterial spores. 4. References: Alcamo. 3rd ed. For the preparation of chocolate agar plates. 4th edition.1 to 18. Incubate at 37oC for 16 to 18 hours.2 u filters are used to remove viruses from the filtrate. READ : Madigan. e. butt and slant-slant. Microbiology : Principles and Applications.06 kgs/cm2) to steam. 3. nutrient agar 6. These may be in the form of tapes. 6. this blood agar is further heated to a chocolate brown color.g. This temperature is reached by applying a pressure of 15 lbs/in2 (1. Test for sterility 16 to 18 hrs. Prentice Hall. Parker. whole non-hemolyzed blood is added after cooling the medium to 50oC. J. 4. Section 4.Membranes with pore diameters of 0. 5. while 0.3 . 1996. 5%-10% sterile. PLATED MEDIA Autoclave at 121oC for 15 to 20 mins. Weigh. Bigger volumes of materials require longer exposure time. Martinko. 3 grams 5 grams 15 grams 1000 grams 2. Most culture media which are heat stable are sterilized using steam under pressure i. N. Jersey. measure the different components e.45 u are usually used to remove bacteria and bigger contaminants. Fundamentals of Microbiology. 7. Generally. then poured into sterile plates. 8. Black. Test for sterility. Form into slant. 1994. autoclaving. nutrient agar beef extract peptone agar distilled water Adjust the pH. Autoclave at 121oC for 15-20 minutes. The following outlines the steps in the preparation of culture media which are heat stable. exposure to a temperature of 121oC (250oF) for 15 to 20 minutes is sufficient to effect sterilization. Indicators for sterilization are used. *For blood agar plates. 6.8 Dissolved in a water bath. 5.3 Sections 19. 2000. Benjamin Cummings.g. Cool to around 50oC* Pour into sterile petri dishes Allow to solidify TUBED MEDIA Distribute media into test tubes.

You're Reading a Free Preview

/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->