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Funding Application for Joint Applied Research Projects


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1. Importance and Relevance of the Technical and/or Scientific Content Identified problem: The role of nutrition for human health is currently one of the priorities of policies and strategies to ensure the quality of life. The idea of food-health relationship, summarized by „we are what we eat”, is not new. Hippocrates postulated that „food must be our cure and the remedies be our food”. New in approaching the concept of healthy eating is the interest for the relation food quality – group of consumers. In addition, in case of foods of animal origin there is a concern for the impact of animal nutrition on food safety and food quality and thus it has been created the dough for the development of the concept Fork to Farm "which is the Activity 2.2., Theme 2 of FP7 KBBE programme”. In such a world in which the interest in healthy eating with more natural products is increasing, the presence and the role of hen egg and chicken meat in the daily basket is a challenge full of opportunities but which requires some changes in the production field of meat poultry and eggs. There was a significant concern to amend certain nutritional qualities in order to respond to the requests of those consumers that take into account the human medical advices or that already have a condition that imposes certain restrictions on cooking. Among the nutrients in eggs and meat, important for human health, there are included the polyunsaturated fatty acids. From these, omega-3 fatty acids are essential for normal growth and development and they can play an important role in the prevention and treatment of cardiovascular diseases, arterial hypertension, diabetes, arthritis and other inflammatory and autoimmune disorders, cancer, etc. Currently, there are many products on the market which are enriched in omega-3 fatty acids. The nutritional path chosen is that of enrichment of the feed diet of laying hens or chickens in omega-3 polyunsaturated fatty acids. To achieve this, there are included in poultry diets, especially, seeds and oils from oleaginous plants. There are situations when the food diets are optimized by including fish oil (tuna, herring, etc.). In both cases, altogether with the addition of feed raw materials rich in fatty acids with double bonds, it must be ensured the necessary amount of antioxidant substances which can eliminate the unpleasant effects of autooxidation of the lipids in feed and in consequence of the lipids in eggs and meat. Antioxidant means "against oxidation." Antioxidants work to protect lipids from peroxidation by radicals. Antioxidants are effective because they are willing to give up their own electrons to free radicals. When a free radical gains the electron from an antioxidant it no longer needs to attack the cell and the chain reaction of oxidation is broken. After donating an electron an antioxidant becomes a free radical by definition. Antioxidants in this state are not harmful because they have the ability to accommodate the change in electrons without becoming reactive. The human body has an elaborate antioxidant defense system. Antioxidants are manufactured within the body and can also be extracted from the food humans eat such as fruits, vegetables, seeds, nuts, meats, eggs 2

and oil. The most used antioxidants in feeds for poultry are: ethoxyquin (1,2-dihydro-6-ethoxy2,2,4-trimethylquinoline), BHA (butylate of hydroxyanisol) and BHT (butylate of

hydroxytoluene), vitamin E. Using these synthetic antioxidants presents also disadvantages for the health of animals and of consumers of chicken meat, chicken meat products and eggs. For instance, BHT, the most used additive from the poultry feeds, prevents antibodies response when poultry is exposed to the action of certain viruses, as it is the virus of Newcastle disease. This effect of BHT in doses of 100-150 ppm in feeds suggest a possible explanation for the losses occurred sometimes at vaccinations performed with live vaccines. Antioxidant effect of vitamin E becomes significant only at high rates of inclusion in feed (200-300 mg/kg), this source being very expensive, which raises the cost price of feed. Supplementary, synthetic antioxidants increase the incidence of cancer, alergies and degenerative diseases of consumers. Solution: At present, when man returns his face to nature, an alternative for synthetic antioxidants is constituted by the polyphenolic extracts from plants. Polyphenols from plants are considered non-nutritive substances, the interest for these compounds being due to the possible beneficial effects on human and animals health. For example, quercitine (a flavonoid) and ellagic acid presented anticancer activity in studies conducted on experimental animals, epigalocatechin gallate inhibits proliferation of tissues of cancerous tumors on the rats skin. The purpose of the project is to value local by-products of vegetal nature, as result of local food industry and food supplements industry, instead of the expensive synthetic compounds and harmful to health consumers of egg and meat poultry, for prevention of polyunsaturated acids peroxidation in the process of obtaining certain healthy food (chicken eggs and breast), enriched in omega-3 polyunsaturated fatty acids and consequently, with antioxidant effect on meat proteins. 1.1. The concept and objectives of the project

1.1.1. Project concept Phyto-additives used in animals feed are plants or products derived from plants which are embedded in animal diets with the purpose of improving animals productivity, or to increase the quality of foods obtained from these animals. There is a very large variety of vegetal compounds from both the point of view of their origin and technological processes (drying, extraction, etc), through which they pass before being used in animals feed. The reason for which, in last decade, the feed phyto-addtitives domain developed is banning at E.U. level, the use of antibiotics in animals feed, as a result of identifying the risk of occurence of bacteria resistance to the action of antibiotics increasingly used in animals feed. The use of phyto-additives with antioxidant role in the case of recipes rich in omega-3 fatty


acids in poultry is relatively new, because there are still studied both their mode of action and the aspects related to the application (local sources, by-products). Adding these vegetal materials in poultry diets raises both from the practical and technological point of view, and from the legal one, three major problems: “what for?”, “in what form?”, “how much?”. Vegetal materials targeted for their antioxidant potential will not be used with curative purpose, but with the goal to ensure and improve maintaining the quality of feed enriched in omega-3 fatty acids, and food (eggs, chicken meat) obtained from poultry fed the mentioned type of feed. Starting from the expression “Feed safety is part of food safety”, the producers, distributors and merchants on the feed market share the safety responsibility of food of animal origin with the players on the food market. Actually, the legislation from the domain of feed production tends to become part of the legislation of producing and marketing food. In this context, but also because of the potential economic benefits, project concept is related to using vegetal by-products from food industry as sources of antioxidants to ensure stability in time to the feed enriched in omega-3 fatty acids for poultry. The major problem raised by the use of vegetal by-products is related to the maintenance of antioxidant properties of the plants of origin. For this purpose, within the project, from the broad range of plants with antioxidant properties (rosemary, oregano, marjoram, seabuckthorn, sage, thyme, grapes, tomatoes, apples, carrots, lucerne, etc.) will be evaluated the compounds with antioxidant action which are found in by-products. Plant antioxidant activity is given by certain specific active compounds, which will be evaluated for each vegetal material under consideration. In the first phase of this project, will be evaluated the by-products resulted from technological processes (in SC Hofigal SA) for obtaining natural food supplements (for example, lucerne leaves contain carotenoids (lutein, zeaxhanthin, astaxhanthin); apple fruits contain flavonoids (rutoside, sinensetin, 3'-hydroxy-5,6,7,4'-tetramethoxyflavone), caffeic acid derivatives (5-caffeoyl quinic acid, rosmarinic acid, chlorogenic acid), catechin, epicatechin, ascorbic acid; grapes contain flavonoids (kaempferol-3-O-gluco-4p" sides, quercetin-3-O-glucosides), procyanidolic oligomers (proanthocyanidins), catechin, epicatechin, epicatechin gallate, resveratrol). Following selection of vegetal materials by tracking criteria related to the local specific and content in substances with antioxidant potential, these must be evaluated from the point of view of antioxidant capacity, pursuing a series of parameters – primary products of lipidic peroxidation: extractions of total phenolics; total phenolic content; flavonoid content; quenching effects of alcoholic extracts on reactive oxygen species, the ability of polyphenols to reduce ion Fe3 +, the ability of polyphenols to chelate transitional metal ions, the determination of the protective effect of substances with antioxidant potential of PUFA in feed: determination of compounds mayors


(peroxides and conjugates diene) and secondary lipid peroxidation in animal feed (TBARS). After selection of optimal vegetal materials, using above mentioned criteria, will be set, according to a calculation algorithm, the inclusion rate in hens and broilers feed. The resulted feed will be subject to the same type of determinations, so that its complete characterization will be obtained. In addition, determinations concerning the maintenance of feed quality for a month period will be established. To study the effects of inclusion vegetal materials with antioxidant capacity in feeds enriched in omega-3 for poultry, tests on animals are necessary to evaluate antioxidant status of poultry and oxidative processes at the level of lipids and proteins: extraction of total lipids; evaluation of conjugated dienes; evaluation of peroxide value; evaluation of thiobarbituric acid reactive substances (TBARS); evaluation of myoglobin concentration and relative concentration of metmyoglobin; evaluation of soluble proteins; evaluation of oxidized proteins. Due to the complexity of the characterization process from the point of view of antioxidant capacity of vegetal materials, feeds and animal products, within the project will be followed the development of a set of chemical methods to determine antioxidant capacity from samples of animal origin (serum, liver, brain, kidney) simple, sensible, selective and well-suited for the types of analyzes. In addition, the bioproductive parameters will be followed and based on chemical and physical analyses the nutritional quality of hen eggs and broiler meat obtained during the experiments will be evaluated. Microbiology and mycotoxicology methods will be used for the quality control of feed, eggs and meat. Heavy metals and microbiological load of manure birds it will be determined. After collating the obtained results a technical documentation will be developed, feed recipes resulted after unfolding the project will be subject to 2 patent applications. 1.1.2. Project objectives Technical and scientific objectives    Nutritional solutions for poultry, innovative and integrative by inclusion of vegetal

by-products resulted from food industry Ensuring of natural alternatives, of vegetal nature, to the synthetic compounds,

expensive, which are used in poultry feed to prevent polyunsaturated acids oxidation Obtaining of functional foods, eggs and chicken meat enriched in omega-3 fatty acids, by procedures and technologies “friendly” to the animal, consumers and environment safety, ERA (European Reserch Area) priority  Creating of a partnership between research facilities and an economic entity, in

order to solve certain problems with social and economic implications, and creating the 5

to determine the effects of by-products (selected at the end of phase 2) used as antioxidant feed additives in laying hens feed. considered waste products. Hofigal Export Import S. “in vivo”. S . University of Bucharest – Faculty of Chemistry (P4). profitable for human health. Experimenting. Dissemination and exploiting of the obtained results. based on scientific research to obtain certain foods.C. University of Agronomical Sciences and Veterinary Medicine Bucharest –Faculty of Veterinary Medicine Veterinary Medicine Veterinary Medicine (P2). 2. enriched in ω3FA. Physical and chemical characterization of by-products (selected at the end of phase 1 to be physically and chemically characterized from the perspective of the antioxidative potential and facility of conditioning for embedding in feed) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed. Multidisciplinary studies regarding methodologies. During these 5 phases of the project. a series of vertical activities will be developed: (i) the coherent 6 PN-II-PT-PCCA-2011-3 . over the 5 stages. The project will be developed in 5 phases: 1. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants in the feed of laying hens and broiler chickens. to determine the effects of by-products (selected at the end of phase 2) used as antioxidant additives in broilers feed. Experimenting. To achieve these objectives a consortium has been created consisting of: the project’s coordinator (CO) . 3.National Research Development Institute for Animal Biology and Nutrition (IBNA). and direct implications on health system by reducing direct and indirect costs associated to people with predisposition for cardiovascular diseases The project objectives matches with the programme PN-II-PT-PCCA-2011-3 objetives for domaine 5. as an alternative to expensive synthetic products constitutes a way to value vegetal materials without economic potential.Faculty of Pharmacy (P3).  Promotion of a healthy lifestyle with role in occurrence of coronary disorders. University of Medicine and Pharmacy Bucharest. from the perspective of the costs of ensuring public health Economic objectives  Using food industry by-products with local specific as sources of antioxidants for poultry feed. “in vivo”. 4. As a result. by increasing the impact of the knowledge.A (P1). 5. we propose that by the interdisciplinary applied research activities to fathom the knowledge of the subject research and to find a solving practical solution.mechanisms to implement innovative solutions resulted from the project   Increase of competitiveness and international visibility of the research team from the project consortium Ensuring the production of sustainable food.

with low costs. climate etc. feed or animal products. (iii) the theoretical applied approach concept through implementation and results valuing. The possibility of providing animal products with high nutritive value. The scientific barrier surpassed by the project consists of obtaining certain functional foods (eggs and chicken meat) cheap and safe.. by the idea of the project. This way. The scientific barrier overcome through the project refers to the elaboration of a protocol of characterizing by-products from the point of view of antioxidant substances and capacity. (ii) the verification and validation of the theoretical concept through design and results of experimental studies. based on which to be possible to decide using them or not. The vegetal materials used as phyto-additives present the disadvantage of a variable chemical composition depending on the collection area. Through the idea of using food by-products is overcome. The barrier overcome by solutions proposed in the project is substitution of synthetic antioxidants with by-products of local food industry in order to obtain certain functional foods (eggs and chicken meat) enriched in omega-3. and for their consumers. Manufacturers and their search for added-value. scientific.3. The growth of the functional foods sector not only represents significant benefits to the health sector. and consumers are encouraged to adopt a healthy lifestyle. but also offers opportunities for processing and manufacturing companies. and economic barriers It is known for a long time that there is a relationship between the food we eat and our health.enunciation of the theoretical concept. and the levels of vegetal materials considered as natural sources of antioxidants. their certification being complicated. the 7 PN-II-PT-PCCA-2011-3 . higher margin products provided key impetus for the growth of functional products. The concept of by-product is related to using a vegetal material from which were extracted a series of compounds considered important in food industry. and from identification of mechanisms by which food modulate metabolism and health. using optimized feed rations with impact on eggs and carcasses quality.1. and with beneficial effect on health constitutes a great advantage for both eggs and chicken meat producers. in a great measure. is surpassed the technical barrier related to the status of “waste” of food industry by-products. Valuing these vegetal materials leads to economic advantages for producers of food. By characterizing vegetal materials and highlighting their antioxidant potential. Technical. by nutritional means. 1. animal breeders are stimulated to invest in the industry producing functional foods. The current concept of functional food resulted from gradual acknowledgment of the fact that healthy diets result from consumption of food with rich content in nutrients.

under the conditions of a world which is changing. healthier and high quality food. Promoting consumer wellbeing amid health ensuring led to the development of certain strategies of obtaining and promoting safer. found also at international level in the requirements of FP7 (“Farm to Fork”) program. 1.2 patent applications applied at OSIM (a feed formula for hens and a feed formula for broilers) wherein the beneficiary is the project’s coordinator. Characterization of vegetal by-products. for laying hens and broilers. their chemical composition being relatively constant (cultured raw materials. leaves.4. obtained in the same conditions). Another solution identified and followed in the present project is using by-products from food industry as natural antioxidants sources. The disadvantage presented by this category is related to the variability of the biologically active plant compounds depending on the soil. and tree bark). antioxidant capacity. according to the used part of plant (for example. economic interest leads to the identification of some natural antioxidants sources with beneficial effect on animals health and with low cost. On one side. Using certain local vegetal materials as antioxidants sources in feeds enriched in omega-3 for poultry. with deliverables . strategic segment of economy. As results will be expected :  the development of a nutritional strategy to optimize feed recipes. Originality and innovative nature Perspective concerns of food industry have in view obtaining foods by “friendly” procedures and technologies. By using certain cultured plants. At present. from the point of view of: compounds with antioxidant action. and nutritive potential. with deliverables . grown in controlled conditions. seeds. contributes to the development of knowledge in a domain without broad bibliographical support. Within vegetal materials. enriched in ω3FA.1. roots. In this category are nutritional methods of influencing the quality of food products of animal origin.scientific barrier related to their characterization. the content of active substances from products may vary a lot.2 licence certifications for byproducts of vegetal origin used as antioxidants in poultry feed wherein the beneficiary is P1 of the 8 PN-II-PT-PCCA-2011-3 . harvesting season and geographical origin. these conditions are met by the plants harvested from the spontaneous flora. and from the economic point of view. on eggs and meat enriched in ω3FA. feeds obtained by their inclusion and of products resultated after tests on animals. is sustained the increasing competitiveness of Romanian food products industry. are valued vegetal materials considered waste. climate. it is eliminated the variability of biological compounds. polution etc.  the decreases of unwanted effects of polyunsaturated fatty acids oxidation from hens and broilers rations. on European market.

added to the diets of poultry. with deliverables – teaching support courses.  producing local functional food (egg. . safe with deliverables – brochures for information and awareness of the consumer wherein the beneficiaries are manufacturers of food industry and non-governmental consumer organizations  a framework methodology for evaluation of the vegetal by-products potential. at national and international level wherein the beneficiaries are young researchers of: CO. P2. P3 and P4  a stimulation of partnerships between research units and private businesses. .USAMV Bucharest and seminars at the Faculty of Pharmacy from UMF Bucharest SWOT Analysis: Viability and risks of the project.the development of a competence center. 4 articles for journals with impact factor and min 6 communication. products added fodders. participation at national exhibitions (INDAGRA.incomplete answers.  the increase of the visibility of competence and expertise of the research teams. AGRARIA) addressed to the business field wherein the beneficiaries are – manufacturers from feed and poultry industry and the business environment. 1.the quality of the work team achieved in previous research projects. young PhD students.the approach of new themes in order to develop them in European programs.special opportunities for the formation of . .the need to re-formulate the experimental Weaknesses . chicken meat) relatively cheap. Opportunities .the rise of the degree of visibility of our approach. roundtable communications. as additives with antioxidant activity.the novelty of the team might come that some of the hypothesis will not confirm. Threats . . research institutes by publishing scientific .the impossibility to certificate the byresearch in mainstream journals. Lumea Satului. partners in consortium with deliverables min. Recolta). Strengths .  a partnership between research facilities and an economic entity with deliverables – a business plan wherein the beneficiary is P1. AGRALIM.the problem of antioxidant capacity evaluation from a multitude of samples.C.project .logistic support for project’s achievement. HOFIGAL EXPORT IMPORT S.2. scientific or nonscientific popularization articles (revues as – Fermierul. wherein the beneficiaries are students of Scientific Society at the Faculty of Veterinary Medicine . with deliverables workshops.A .S. . State of the art 9 PN-II-PT-PCCA-2011-3 .

generally recognised as safe additives. At present.According to the traditional concept of nutrition. Among these can be mentioned: oils rich in ALA (canola. used in producing bakery products. Synthetic antioxidants 10 PN-II-PT-PCCA-2011-3 . More than half the calories found in eggs come from the fat in the yolk.5 billion dolars. Eggs can make a valuable contribution to a healthy. healthful diet rather than on single nutrients or functional components. diet not only helps to achieve optimal development and health. it is important to educate the consumer on the importance of a balanced.4 billion dolars). they would be open to functional food components if added to or present in foods they already eat and like. However epidemiological. mayonnaises. or DHA from algae led to the enrichment of eggs in omega-3 fatty acids). the lipidic peroxidation process attracts the necessity of using synthetic antioxidants in feeds. the “big four” European markets being UK (2. flax seeds. eggs (including in poultry feed of fish flour. In its turn. Among the different products delivering essential nutrients to the body. and soy). Natural products. Japan is the world's largest market at 11. flax oil. margarine and salads dressings. experimental and clinical studies have shown that certain types of food and specific food components can affect a variety of body functions and provide health benefits. were used by nutritionists to alter the diet of laying hens and reduce cholesterol content of eggs. According to an Euromitor survey. France (1. while unmotivated consumers were skeptical of information on diet and health.2 billion dolars). milk. Therefore. People on a low-cholesterol diet may need to reduce egg consumption. farmed fish. 1995). the primary role of the diet is to provide adequate quantities of nutrients to meet metabolic requirements and maintain optimal health. Diet plays an important role in maintaining health. including manufacture of bread). there are many products on the market which are enriched in n-3 fatty acids. but it may promote better health and play an important role in disease prevention by reducing the risk of certain chronic diseases. The question of cholesterol in eggs – a previous concern that resulted in lower consumption – is an important subject.7 billion dolars. However.4 billion dolars). balanced diet in that they provide high quality protein and a number of vitamins and minerals. an egg has arguably a special place. bakery products (flaxseed flour and capsulated fish oils (Andersen. and Italy (1.5 billion dollars while the European market is less developed with an estimated market of 7. A survey conducted for the International Food Information Council found that. infants formulas. then US is the second one market with around 10. meat and products from poultry.6 billion dolars). perilla. Germany (2. Adding oils rich in polyunsaturated fatty acids (PUFA) to poultry diet has the effect of increasing the occurence of the lipidic peroxidation process. Health professionals can help consumers develop a positive attitude toward functional foods.

colorants. nutritional additives . Plants polyphenols are considered non-nutritive substances.2. but in the case vitamin E should fulfill the role of antioxidant must be added a quantity of approximately 200-300 ppm. antioxidants added in feed are of synthetic nature.vitamins. in Romania the most frequently used being vitamin E. Tagetes Patulus) on the stability of feeds enriched in omega-3 fatty acids destined to poultry (Criste et al. 2008a. 2008b). food enzymes. in Romania. aminoacids. 2008d). zootechnical additives . it was studied the effect of plants (Marigold (Calendula Officinalis). State of the art at national level At present. sensorial additives . 2008a) studied the antioxidant effect of vitamin E using different inclusion rates in hen feed and found that the feed supplemented with 250 ppm vitamin E increased the shelf life of the feeds. Panaite et al. and on zootechnical performances of animals. Antioxidant capacity of feed enriched in oregano and cranberries was studied in view of 11 PN-II-PT-PCCA-2011-3 . antioxidants. contain numerous bioactive components. Studies about the effect of Selenium on stability of feeds for pig and chicken.1. 2008. 2007. fatty acids.protect fatty acids. as well as other compounds from feeds destined to poultry. In compliance with the amendment of European Parliament with respect to the ordinance 1831/2003. To cover the necessary vitamin E for laying hens and broilers. Nettle (Urtica Dioica). 2008c. but have also negative influence on their health and on the health of consumers of chicken meat and eggs.and prebiotics as stabilizers of intestinal flora. Panaite et al. stabilizers. were conducted under the direction of teams of scientists from Romania (Panaite et al. namely: influence the taste and flavor of feed. must be added in their feed a commercial vitamin premix which has to contain approximately 30 ppm.pro. 1. additives used at ensiling. the interest in these compounds being due to the possible beneficial effects on human and animals health. A team of researchers from Romania (Panaite et al. published an article regarding the adjustment of quality control of feed enriched in omega-3 and vitamin E. Because animal organism can store vitamin E. five categories of food additives are favored: technological additives . Ropota et al. and coccidiostatics and hystomonostats. Plants improve use of feeds and have favorable effects on poultry (laying hens and broilers).preservatives. as it can happen in case of using vitamin E as antioxidant in feeds with fatty substances. mineral components. Panaite et al. An alternative to the synthetic antioxidants could be polyphenolic extracts from plants. In Romania. stimulte animal organism and have a positive effect on the color of egg yolk and fowls. when administered in excess emerge also its harmful effects. aromatic substances. Use of plants in poultry feed is economic and natural. emulsifiers.

Tang et al. Smet et al. 2000. Radwan et al. Plantago major. Echinaceae herba. At the national level.2. rosemary.2. grape seeds and oregano. and in the muscle tissue post-mortem (in chicken chicks.0% savory. in pigs.donor activity. protocatechic. proantocyanidines and resveratrol. in chicken chicks. Most natural antioxidants are fat-soluble phenols. Phoeniculus sp.5% Curcuma longa were used. caffeic and rosmarinic) to the phenolic diterpenes (carnosol. Ahn et al. Rojas and Brewer 2007. rosemary. 2008). or 0. In another study. green tea. phenolic acids. This antioxidant activity is due most time to the phenolic acids (gallic. antioxidative status and immune 12 PN-II-PT-PCCA-2011-3 .. and kaempferol) and to the volatile oils (eugenol. carvacrol.their use in experiments on pig (Papuc et al.. to the flavonoids (quercetin. Some plant pigments (antocyanine and antocyanidine) may chelate metals and give away H towards the oxygen radicals. in chicken chicks. 2008a. Brenes et al.1. sage. thus being extremely efficient antioxidants. may improve layer performance. 2009). The results have shown that the supplemental plants as natural antioxidants used during the laying period. 2008c) were implemented in poultry industry feed recipes designed to obtain eggs enriched in omega-3 fatty acids using feed plants and oils rich in polyunsaturated fatty acids. Artemisia absinthium. The literature has several studies showing the antioxidant capacity of some plant products used in poultry feeding. determined the antioxidant effect of savory. 2008). Sasse et al. Alia et al. particularly when 1. The savory and rosemary also had a positive effect on the oxidative stability of the stored eggs.. 2007. Melissae folium) from the local market were analyzed for total antioxidant and scavenging capacities for including in poultry feed (Stef et al. 2008. Chelidonium majus. rosmanol and rosmadial). . epictechins.. naringenin. a total of eleven medicinal herbs (Rhamnus frangula. Hasty et al. tymol and menthol). 2005 studied the effect of Ligustrum lucidum and Schisandra chinensis on egg production.. The plant extract are generally used for their aromatic properties. 2003. but also often have an intense H.. 2003) and in vivo in plasma (in rats. 2010). Many of these extracts and their purified components have in vitro antioxidant activity (Blazovics et al. catechin. oregano.. 2009). 2008b. Epilobium montanum. Taraxacum officinale. Crataegus monogyna. Malva silvestris. all of them contributing to the antioxidant activity. such as phenols from the rosemary. 2002.. Maa et al. carnosic acid. thus slowing oxidizing by two mechanisms. oregano and şi Curcuma longa on the productive and reproductive performance of local hen breeds and determined the oxidative stability of the yolk from eggs stored at room temperature (16oC ± 2). Criste et al. The tea and the extracts from grape seeds and peel contain catechins. State of the art at international level A large number of herbs and aromatic plants contain compounds that can be extracted and used in foods to prevent oxidation (Lee and Shibamoto 2002. following undertaken studies (Criste et al.

Ligustrum lucidum and Schisandra chinensis reduced significantly the heart. Polat et al. 2006). grapes seeds. The natural tocopherols and the rosemary showed the best effects at an inclusion level of 200 mg·kg–1. investigated the effect of the antioxidants from the linen oil and plant extrscts (rosemary.05). green tea. Racanicci et al. It was concluded that green tea powder inclusion in the diet for layers can reduce the cholesterol content and TBA value of the egg yolk. n − 3 PUFA (flax seed and marine algae) and rosemary may be used to hens fed diet without affecting the sensory properties of eggs. Vossen et al. flax seed). α-TOC status. grape seed.status of the layers under heat stress. 2011 studied the effects of the watery mate (Ilex paraguariensis) extract given to broiler chicks. reproductive performance and egg quality during storage and its content of cholesterol it was studied by Zeinab et al. in addition to improving the productive and reproductive performance of both hens and roosters. implying its potential effect on egg quality parameters. oxidative stability. tomatoes) on the antioxidant status of the plasma in chicken chicks fed on feeds rich in polyunsaturated fatty acids. (2010) investigate the effect of dietary antioxidants and storage on fatty acid profile. In onther work (Parpinello et al. The results show that 1% Ligustrum lucidum and Schisandra chinensis has beneficial effects on egg production under heat stress (p<0.. Hayat et al. The oxidative status and lipid oxidation of broiler plasma was not affected by the dietary natural antioxidants. and vitamin E concentration of n3 fatty acid-enriched eggs.. liver. The study also used a diet with synthetic antioxidants with or without alpha-tocopherol acetate (200 mg/kg feed). The effect of adding green tea and its aqueous extract as natural antioxidants to local laying hen diets on productive. it was report the first sensory characterization of eggs and products containing eggs obtained from hens diet based on grape and algae plus vitamin E and rosemary extract. These studies demonstrate that the level and type of antioxidants and duration of egg storage significantly affected the fatty acid profile. rosemary. (2010).. The extracts were added as such or in combinations ion the diets with 4% linen seeds oil. volatile rosemary oil and alpha-tocopherol acetate (vitamin E) on serum parameters using a soybean-corn based diet. but the activity of the antioxidant enzymes changed slightly. The results suggested that vegetable lipids (palm butter.. green tea. Smet et al. 2008 monitored the influence of the supplementary natural antioxidants (natural extracts of tocopherols. and oxidative stability of chicken eggs. especially during storage. 2011 worked on broilers too (800 day-old male Ross-308 chicks) monitoring the effects of the supplementary rosemary. The concluding was that Rosmarinus officinalis and its volatile oil increased serum SOD activity and affected positively the oxidizing mechanisms. grapes and tomato seeds) on lipid an protein oxidation in the broiler meat. serum end yolk malondialdehyde. The extract was added to the drinking water during the final 14 days before 13 PN-II-PT-PCCA-2011-3 . 2011.

There has been a tremendous increase in the research related to reactive oxygen species (ROSs). The determination of the active compounds which are antioxidants is also important. Ho. 2011. Conventional methods for the determination of antioxidant capacity can be roughly classified into two types: assays based on hydrogen atom transfer (HAT) reactions and assays based on electron transfer (ET) (Huang. 1995) due to postharvest deteriorative reactions. flavor.5:48. 2008. colour. but it almost disappeared after 7 days. The phytogenic additives vary a lot in their in vivo effects on animals. after refrigeration for up to seven days. to antioxidants and to oxidative stress and the number of papers dedicated to these subjects has been increasing exponentially in the last two decades. 2007. Free Radical Research. This variation is due to their different composition and to the biological effects of these feed additives. The best result was noticed during the first day of storage. The oxidative stability of the meat preparations made from the control and experimental groups was checked. which many times and mixtures. oxygen radical absorbance capacity (ORAC) and total radical trapping antioxidant parameter (TRAP). 2005).slaughter. with more than 50% reduction in TBARS. working on day-old male Arbor Acre chicks. The mixture increased the antioxidant potential of the broiler breast meat during refrigeration. texture and safety of foods. This is why the determination of the active compounds allows the efficient use of this type of phytoadditives.C).) and B) some journals dedicated exclusively to this topic (Free Radical Biology and Medicine. So much work has been done in this area. where it is a major causes of chemical spoilage (Colbert et al. Oxidation can affect foods. The data on the way in which the marketed phytogenic additives work is rather vague. 2007.5:3. Defense mechanisms against the effects of excessive oxidation are provided by the action of various antioxidants and the need to measure antioxidant activity is well documented. Antioxidants and Redox Signaling. Guteridge. 2008. Japanese honeysuckle and goldthread (48.0). Donga et al. increased the antioxidant potential of the broiler breast meat with polysavone (natural alfalfa extract). that it is worth mentioning: A) some relevant books published in the field (Halliwell. HAT-based assays include inhibition of induced low-density lipoprotein auto-oxidation. when the oxidative rancidness was very high in all samples. ET-based assays measure the capacity of an 14 PN-II-PT-PCCA-2011-3 . the result was significant after 3 days too. The evaluation of the biological effects is difficult if the composition of the tested substance is not stated or variable. Yu. Shahidi. who used a mixture of blackberry leaves. It is estimated that half of the world’s fruit and vegetable crops are lost (Martinez et al. 1991) resulting in rancidity and/or deterioration of the nutritional quality. These results are in agreement with those of Jang et al.

Accelerated stability tests. The usual substrate for the determination of the conjugated dienes includes any substrate containing polyunsaturated fatty acids. The MDA is reacted with thiobarbituric acid (TBA) to form a pink pigment that is measured spectrophotometrically at 532-535 nm. with oxidation being initiated by the adition of copper ions. The TBARS procedure is widely used even though the reaction is not very specific and reaction conditions have a significant effect on the colour development. The electron spin resonance (ESR) spin trapping assay could be used for antioxidants determination.) on plant biophenols.antioxidant in the reduction of an oxidant which changes color when reduced. possible addition of iodine across unsaturated double bonds leading to low results. e. AAPH or DDPH or the application of heat.g. Measurement of free radicals. butanones. End-point detection has been based on measurement of fluorescence inhibition. pentanones. Diene conjugation resulting from lipid oxidation (Heinonen et al. ketones (e. conductivity or diene conjugation. Methods have been developed for measuring the antioxidant activity as the ability to scavenge free radicals generated in aqueous and lipophilic phases. 1999..g. iron ions. hydrocarbons and saturated and unsaturated aldehydes such as hexanal. Stability tests for edible oils commonly involve accelerated deterioration tests. In the following we shall shortly describe the main methods for measuring antioxidant activity related to lipid oxidation. Measurement of hexanal and related cend-products. o-diphenols contribute over 50% to the stability of virgin olive oil. octanones). There is intense interest in identifying natural antioxidants for use in foods and there has been considerable focus (Bonilla et al. ET-based assays include the total phenols assay by Folin-Ciocalteu reagent (FCR). chemiluminescence 15 PN-II-PT-PCCA-2011-3 . Guteridge. 1998) is now commonly used as an end-point for determining the antioxidant activity of a sample. Decomposition of the primary products of lipid oxidation generates a complex mixture (Halliwell. 2007) including epoxides. The TBAS assay is now the most commonly used method to detect lipid oxidation.2-diphenyl-1-picryl hydrazyl radical) method. Following oxidation the end-point is determined by measuring parameters such as peroxide value. Peroxide value (PV) Limitations involving this procedure are well recognized and include poor sensitivity and selectivity. assay using a Cu(II) complex as an oxidant (CUPRAC) and DPPH (2. None of the methods mentioned above has general applicability. This procedure measure the MDA formed as the split product of an endoperoxide of unsaturated fatty acids resulting from oxidation of a lipid substrate. These accelerated tests are specific to the analysis of oxidation in food with results usually expressed as an induction time. trolox equivalence antioxidant capacity (TEAC) ferric ion reducing antioxidant power (FRAP). Diene conjugation. oxidation of iodide by dissolved oxygen and variations in reactivity of different peroxides. Thiobarbituric acid reactive substances (TBARS) assay.

M. whose inventors are Durand D. Comparison between our product that we aim to develop and other products available worldwide There are lots of scientific papers regarding this subject. [Fr]. Date: Aug. 265702. have an application patent on a cost –eficient additive derived from a plant material originating from olive (Olea europaea L. No. vegetal byproducts. 1. Hvidovre (DK) and T. Another application patent “Composition and methods for enhancing the antioxidant status of animals” Pub. March 2011 The patents and projects related to the idea of the project refer to the use of natural sources in animal feeding. Vitamin C. Gladine C. taurine. but not so many patents on animal nutrition area. 2005). [Fr]. 16 PN-II-PT-PCCA-2011-3 . The information shows that the project is not in conflict with any of the ideas already presented in the literature. etc.3. 2010. G. Flavonoids in fruits and vegetables : their impact on food quality.:US 2008/0187620 A1.absorbance. Bronby Strand (DK). whose authors are K. K. ROSs form with the CL reagents excited species that generate CL radiations.) effective as addition to feed. The most widely used CL reagent to trap oxidants and convert weak emissions into intense. et al. nutrition and human health (FLAVO). Date: Jan 28. Elgaard. prolonged. No. in “Natural antioxidative additive for feed and products use for humans”. [Fr]. 2008. Topeka (US) . Picaud T. Date: 2008-03-26. used as a substitute for traditional growth promoters and as a substitute for synthetic antioxidants. Bauchart D. K. Medina B. Recoquillay F. Pub. Several national and international research projects approached topics in the domain of this project: New Phytogenic Product Line for Pig Production (PHYTOGENIC ADDITIVES QLK5CT-2002-70928). when breeding and producing livestock. Carthagen IN (US) and R.2. they have invented a compositions suitable for enhancing the antioxidant status of animals containing methionine. [Fr]. The application patent „Plant-based natural antioxidant additive intended for animal nutrition” Pub. whose autors are B. and vitamin E. Gruffat D. Pub.: US 2010/0022636 A1. and stable light emission is luminol (Prior. The CLM methods are generally more sensitive than the conventional methods used for ROSs and for antioxidant determination. Yamka. In principle. Plants and their extracts and other natural alternatives to antimicrobials in feeds (REPLACE FOOD-CT-2004-506487). [Fr] have invented an antioxidant additive herbal extract comprising extracts of more thant 10 herbs used preferably in ruminat’s feed. As for patents. None of these patents or projects analyses Romanian local sources. [Fr]. which have shown carcinogenic characteristics. Pub. [Fr].7. Nielsen. Quality and Safety of Feeds and Food for Europe (QSAFFE – FP 7) collaborative project No. Friesen.

( 2008) highlights the high antioxidant activity of polyphenols from Sea buckthorn fruits (Hippophae rhamnoides). Macroscopic examination to determ the morphological and anatomic characteristics.. CO’s team has contributions concerning the enrichment of laying hens diet in ω: 3-PUFA (Criste et al. 2008). Tamas et al (2007). zearalenone in samples by ELISA . microscopic examination. has a paper regarding antioxidants of plant origin. 2010).1.Pharmacognostic analysis” comprises qualitative and quantitative tests in order to verify or to establish the identity. ash (gravimetric method). The main goal of this studies were to evaluate the potential of a layer diet with different levels of linseeds or linseed oil to produce ω: 3-PUFA-enriched (alpha-LNA and DHA) eggs and to monitor layer performance. Panaite et al. fatty acids (gas chromatography method).4. Microbiological investigations are represented by determination of total number of germs. fumonisin. cholesterol (gas chromatography method). assay of active principles. Physical and chemical methods (according to Regulation (EC) no. Criste et al. An adequate methodology must be used for to analyse a vegetal raw material and organic matter. Scientific/technical contribution: The contribution of the project research teams at state of the art is diverse. Haugh unit. soluble-substances. (2010) have contributions to determination of some plants active components.2. There were good results when were used 5% linseed in layer diets.. yolk color intensity and freshness egg. crude protein and crude fiber (Kjeldahl method). deoxynivalenol. swelling index..enzyme-linked immunoassay method. chromatographic analysis. Quality parameters: loss on drying. Aslo a common collective of authors (CO and P2) has published a paper on the effect of some mineral and phytogenic additives.( 2008) has a study regarding relation between dietary vitamin E and yolk cholesterol. (2011) have characterized some plants extracts with antioxidant traits. whites pH and yolk pH (electrometric method). purity and quality of a herbal drug.. ochratoxin A. Alfatoxins B1. Gird et al. Identity parameters: macroscopic examination. total number of fungus on poultry feed and feces and by determinations of Salmonella spp on eggs and feces. Papuc et al. Popa et al. total number of Escherichia coli culture. qualitative chemical analysis. Qualitative chemical analysis for confirming the presence of a chemical compound (which may be 17 PN-II-PT-PCCA-2011-3 . total number of coliforms. The paper has common collective of authors from CO and P1. trace elements (atomic absorption spectrometry). 2009. The physico-chemical analysis laboratory is accredited ISO 17025. . Toxine T2. Mycotoxicological investigations highlight the concentration of mycotoxins as Afla − aflatoxins. total ash and ash insoluble in hydrochloric acid. Purity parameters: foreign matter. rich in polyphenols (Papuc at al. egg thickness. (2009) and Tudor et a. 152 / 2009) allow the determinations of the dry matter (gravimetric method). used in fodder premixes to obtain consume egg with new qualities of functional food.

. Alia. spectrophotometric determination of flavonoid content. G. SauraCalixto. Horcajo. Evaluation of antioxidant activity of plant polyphenols: spectrophotometric determination of total phenolic content (TPC) ( based on the reaction of FolinCiocalteu reagent).. I.1999. (iii) Optimization of the feeding factors to produce consumer eggs enriched in omega 3 polyunsaturated fatty acid. and Medina. A. Szentmihalyi. These were: (i) Development of Natural Alternatives to Anti-microbials for the Control of Pig Health and Promotion of Performance (FEED FOR PIG HEALTH – FOOD-CT-2004-506144). Spectrophotometric methods. M. F. Acta Biologica Szegediensis. C. (2008b. Centeno. HPLC methods). evaluation of peroxide value (PV).. Assay procedure for chemiluminescence emission kinetics and for quenching effects of alcoholic extracts on reactive oxygen species: the annihilation of hydroxyl radical. F. Nutriton Research 23. evaluation of myoglobin concentration and relative concentration of metmyoglobin. evaluation of soluble proteins. K.. J. Effect of grape pomace concentrate and vitamin E on digestibility of 18 PN-II-PT-PCCA-2011-3 .The researchers from the coordinator’s team were involved in several projects investigating then use of plants in animal feeding. Swelling index expressed a high content of mucilage in an herbal matter (Titrimetric methods. L.. Brenes. Arija.. Blazovics. (iii) Interdisciplinary research on the use of Morus sp. Goya. new functional food on the Romanian market (CEEX 22/ 2005). Extraction of phenolic compounds from red grape marc for use as food lipid antioxidants. S.. 1251–1267. 1–4.not the main active principle). Bravo.. evaluation of oxidized proteins 1.project director Criste Rodica Diana.. evaluation of thiobarbituric acid reactive substances (TBARS).. 66(2). A. following the investigations by Criste et al.. Viveros. Preliminary results . 2008. Superoxide anion scavenging activity. 47. Bibliography: 1.. C. 2008c) feed formulations have been implemented in the poultry industry. A. using oleaginous seeds high in polyunsaturated fatty acids. the annihilation of singlet oxygen.. A. Bonilla. Evaluation of lipid peroxidation in meat: evaluation of conjugated dienes.. Sa´yago -Ayerdi. 2003. Merida. Food Chem.2.. I.. Gon˜ i. the annihilation of hydrogen peroxide. in poultry production. GC. 209-215. 4. the annihilation of hypochlorite anion. for the production omega 3 fatty acid-rich consumer eggs. Mayen. L. Qualitative chromatography: TLC. M. Lugasi. 2003. At the national level. Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. 3.. 2. M. Reducing power of the natural polyphenols of sempervivum tectorum in vitro and in vivo. (ii) Optimization of the compound feed formulation in terms of vitamin E/selenium/fatty acids ratio to improve broiler carcass quality (AGRAL 132/ 2002) – project director Criste Rodica Diana . peroxyl radical scavenging activity.5. HPLC.

2008. T. D. I.. G. Donga. T.. F. vol. Maa. 9 – 11 May 2009. 1995. 2011.. D. British Poultry Science.. Pasha.M. 52(3).. pag. 50(17). 53. 2005.. Effects of moderate (5%) levels of linseed in layer diets.. folium. 1248-1250. J. 87. Food Sci. Jang. Oxford University Press..I. supplement 1. 13. Determination of antioxidant potential of volatile extracts isolated from various herbs and spices. B. Lia. Poult Sci. A.Criste.. K. M. Dua. Hasty. 80. J. R.. Martinez. M.. Van Heuten. D.. Comparative Chemical Study of Passiflorae incarnatae flores. 18. 14.. I. Trends Food Sci. Poultry Science. and Hopia.. Atena. Gîrd et all. Cherian. 11-21 7. D. “The Chemistry Behind Antioxidant Capacity Assays”... Oxford. 2002. F. L.. antioxidant status and immunity of laying hens during heat stress. J. Archives of Animal Nutrition..W. 2009.A. R.R. “Free Radical in Biology and Medicine”. W. J. & Xua Q. Lee.F.. Improvement of egg qality by using diets optimised in terms of polyunsaturated fatty acids (PUFA). K. 2009. Lee. C. Gaob. Shin. Criste. and Jo.. 2008. 12. Q. Agric.... 2382-2389. Stoica... 14th Panhellenic Pharmaceutical Congress.L. A. See. Food Chem.J. 2010. Huang. Oxidative stability and lipid components of eggs from flax-fed hens: Effect of dietary antioxidants and storage. 17.. Khattak.polyphenols and antioxidant activity in chickens. B. Antioxidant Activity of an Ultrafiltration Permeate from Acid Whey. Effect of vitamin E on improving fresh pork quality in Berkshire and Hampshire-sired pigs. J Agric Food Chem. N. J. Technol. Lee. Lehtonen. 195-200. 25-31.. 8. 6(6). Z. T. 1285–1292.. 15. 59(6). Z. Poultry Science. 10. Antioxidant Activity of Berry and Fruit Wines and Liquors. 9. Colbert.. 302-309. 19 PN-II-PT-PCCA-2011-3 ... X. & Zhanga. E. 46 (1)... Effect of Ligustrum lucidum and Schisandra chinensis on the egg production.. D. 4th ed.. A. 1841-1856. D. 11. Tonga. J. 6.C. X. H. J. M. S. J.. D. D. Ropota. 4947–52. J. Journal Animal Science. E. Shibamoto. Antioxidative Potential of Raw Breast Meat from Broiler Chicks Fed a Dietary Medicinal Herb Extract Mix.. M. J. B. 3230–3237. 56(5). Shana. 439-447... 87. Archiva Zootehnica 12:3.. Pricop. Halliwell. 109. Rachieru.... Lee.. 5. 2005.. Hayat. Sua.V. J. P. and Ionita.64. 1991.D.. 2007. 16.L. 1998.. 2002. caulis and herba. Effects of dietary polysavone (Alfalfa extract) and chlortetracycline supplementation on antioxidation and meat quality in broiler chickens. Ou. Prior. and Whitaker.. Liu. and Decker. Larick. K..M.. 89 . H.B. Guteridge. Heinonen. A. R. Food Chem. and Jabbar. C. Panaite. The biochemistry and control of enzymatic browning. 2009. 307–316. M. Agric. D. Panaite.M. 11-21.. Chena. Ciurescu. M.G. World’s Poultry Science Journal. Songa. T.

Racanicci. ISSN 1454-7368). Meluzzi. 21. R.. Iasi. Nicorescu. Effect of using 250 ppm supplemental vitamin E in omega-3 PUFA-enriched diets for layers. Criste. R. 2005. published on line 23 februarie 2011. Ed. 2011.. pp. and Fayek. T...64. D. 2011. 23-30. 392-394.. Raducanu. Antioxidant activity of polyphenols from Sea buckthorn fruits (Hippophae rhamnoides). A. Papuc. M.. Wu.. D..M.. Bulgaria.. 39(1). R. L. Biol Environ Sci. The effect of some mineral and phytogenic additives. N... 2008.. Cismileanu.. 30. 61(10). SERBIA 23.D.. T.6] 24. European Food Research and Technology. Novi Sad. 232. J. [ISI 0. Parpinello. 26.. Food Chem. 59(4). Serum Biochemical Profile of Broiler Chickens Fed Diets Containing Rosemary and Rosemary Volatile Oil. E. 2008. X. 47-52. Serdaru. Versari. and Skibsted. 28..17). Total Antioxidant Activity.P. U. Polat. Food Prop. N.M. upon lipid peroxidation process. 2006. Nicorescu.. Panaite. I International Congress on "FOOD TECHNOLOGY. 91-95.S. Ion Ionescu de la Brad. 51. QUALITY. 5(13). 2010.H. Criste. Prior. Journal of Animal Science. [ISI 0. A. and Eren. Lucrari stiintifice seria Zootehnie. 2008. 13-15 Noiembrie. J. ISSN 0034-7752. C. 598-603.Academia Agricola din Sofia (ISSN 0514-7441). Qota. Revista de Chimie Bucureşti. C. AND SAFETY". Buissa.. Untea. 22... Menten. Data on yolk vitamin e and cholesterol from layers fed dietary oil extract of tagetes patulus.. 2007. C. Ciurescu. M.. M.. Diaconescu. Panaite. R. 3. Durdun. 2011. and Criste. A. Radwan. vol. Panaite. A. Rachieru. N. 53. 920-924. Hassan. W.. Rachieru.C. R. Schaich.19. rich in polyphenols.. F. Ionescu. C. Mate (Ilex paraguariensis) as dietary additive for broilers: performance and oxidative stability of meat. Agric. Sirri.. 29. (B+. J.. T. D. L. V.6] 25. pag. S. R.. J. G. Studies on the effect of the dietary selenium source for fattening pigs (60-110 kg) on pig performance. 27. Yesilbag. 2008.. Alencar. 2008. Ciurescu. C. V.... T. D.. 655-661.. “Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements”.. Effect of Natural Antioxidant on Oxidative Stability of Eggs and Productive and Reproductive Performance of 20 PN-II-PT-PCCA-2011-3 . „Sensory evaluation of egg products and eggs laid from hens fed diets with different fatty acid composition and supplemented with antioxidants” Food Research International.. World’s Poultry Science Journal (ISI 1. A. R.. 4290-4302. Raducanu.D. Popa et al. A. F..A. F. Comparative data on the use of different selenium sources in the layer diet. D. 20. and Criste.. Tallarico.. M. C. H.. Int. C. K. supplement 1.. Panaite. Revista de Chimie. C.. ISSN 0034-7752.M. si Criste. 115. Bercaru. R..L.. Crivineanu. Phenols and Flavonoids Content of Several Plant Extracts. Papuc.

. Ropota. Brewer.. Kerry. S.. Shahidi. Washington. R. J. 2011. Haak. Panaite. refrigerated beef and pork. S282–8.46.... 134-150. and Helmy S. Buckley. 41(1).. L.. 3(12)... Smet. Raes. Physiol. Tudor et all. 20-23 August. Ionescu.Timisoara.. Effect of adding green tea and its aqueous extract as natural antioxidants to laying hen diet on productive. G.. Editors. A. Rojas. S. Vossen.. J Food Sci. 2007. 37. Rojas. S. 2008. 1121-1149. P. 42. Sci. Smet.. Poult.. and De Smet. F. K. K. Adaptation of the quality control of the polyunsaturated fatty acids and vitamin E-enriched feeds to the European StandardsLucrari Stiintifice zootehnice si biotehnologii. used in fodder premixes to obtain consume egg with new qualities of functional food. International Journal of Poultry Science. 2008a... Effect of natural and synthetic antioxidants on oxidative stability of cooked. 31. Colindres. K. P. 2007. 33. Editor. M. “Antioxidant Measurement and Applications”. Effect of natural antioxidants on oxidative stability of frozen. Egypt. Hassan.. Brewer. Tamas. Romania 39. Lipid and protein oxidation of broiler meat as influenced by dietary natural antioxidant supplementation. 541-547. Effect of natural antioxidants on oxidative stability of cooked. 2007.. American Chemical Society. ACS Symposium Series 956. Urticaceae. Sasse. R. Nutr.. K. 2008. Brewer. T.. Ntawubizi. L. Smet. M. E. Arnouts. 87. E. reproductive performance and egg quality during storage and its content of cholesterol. Raes. G. Tang. De Smet. Arnouts. M. liver and heart. M. Criste... J. 36. C. K. 2000. 2010. Mazilu. Huyghebaert.. M. T. J Food Qual. 2009. 2008. Wiley-Interscience. Lipid and Protein Oxidation of Broiler Meat as Influenced by Dietary Natural Antioxidant Supplementation. 1682-1688.. Poult Sci. Poult Science. A.. Raes. Anim. Acta Medica Marisiensis. 30 (4). 2008. 1682–1688. R. J Food Sci. G. 38. Abd El-Salam A. 87. F. Zeinab Abdo... A. Ho. Contribution to phytobiological study of Urtica dioica L.T.. 35.. Haak.. 198-205. A. Huyghebaert. 41. S. 43. 7 (2). M. S. Anim. 72. P.D. 285–290. K.. Antioxidants of plant origin. 56 (1). Criste.. Arnouts. M. 34.. S30–5.173–88. Panaite.. frozen pork patties. Sheehan..V. D. S.... T. L. 74(1). Rachieru... Z.. Yu.. vacuum-packaged beef and pork. S..S. Meat Science 56. 2010.Laying Hens.. D. Dietary tea catechnins and iron-induced lipid oxidation in chicken meat. Huyghebaert. Sinaia. S. 95. “Wheat Antioxidants”. De Smet. 32. 40.S. Effect of dietary antioxidant supplementation on the oxidative status of plasma in broilers.. A.. Morrissey. First Conference with International Participation. 21 PN-II-PT-PCCA-2011-3 . 43 .

. P1. P2. P1. P4 19 27 5 CO. P3. “in vivo”. P3.. P4 9 18 4 CO. P2. “in vivo”. P2. enriched in ω3FA Experimenting. P4 4 8 3 CO. Multidisciplinary studies regarding methodologies. P1. n-1) (2 . P3. to determine the effects of by-products (BYPROD’S) used as antioxidant additives in broilers feed Dissemination and exploiting of the obtained results Involved partners Start End month month (1 . to determine the effects of by-products (BYPROD’S) used as antioxidant feed additives in laying hens feed Experimenting. P1.. P1.Table: Phase list Phase Phase title no. P2. P3.. n) 1 CO. P3. P4 1 3 2 CO. P4 28 34 22 PN-II-PT-PCCA-2011-3 . P2. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants in the feed of laying hens and broiler chickens Physical and chemical characterization of by-products (BYPROD) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed.

in poultry nutrition. Study of technical and economic feasibility on the opportunity of valuing vegetal byproducts from food industry. on lipid peroxidation of PUFA from poultry feed. with compliance with the legal regulations in the domain : P1 – Will perform the technical and economic feasibility study. Methodologies and modalities of characterization and assessment of the antioxidant 23 PN-II-PT-PCCA-2011-3 . CO – Documentation on the norms and regulations concerning the use of phyto-additives in animals feed Task 1.Obtaining food (egg. which attains the estimated nutritional needs and food preferences of healthy and dynamic consumers.3.Setting the methodologic framework for evaluation of the vegetal by-products potential.Table: Phase description (for each Phase-max 2 pages) Phase no. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants chickens Involved partners Personmonths Start month End month CO 6 May 2012 July 2012 P1 16 P2 2 P3 12 P4 1 Total 37 in the feed of laying hens and broiler Objectives . safe.1. on the antioxidant status of poultry and on the oxidation processes of lipids and proteins from the poultry meat. 1 Phase title Multidisciplinary studies regarding methodologies. in the context of regulations in the domain of feed additives Description of work (possibily broken down into tasks) and role of participants Task 1. with antioxidant potential. nutritionally rich. chicken meat) relatively cheap. .The technical and economical analysis on the opportunity of practical application/ valuing byproducts resulted of food industry and food supplements industry of the partner economic entity (P1) as feed raw materials for poultry. P3 – Study on the component chemical substances which give the antioxidant characteristic of vegetal materials Task 1. Screening of vegetal by-products with antioxidant capacities: CO – Updating published data on the nutritional solutions of feed enrichment for laying hens and broilers with omega-3 polyunsaturated fatty acids. as additives with antioxidant activity. and the field regulation) . organs and eggs. P2 – Study on the products of vegetal/botanical origin used as antioxidants in animal nutrition. added to the rations of poultry (laying hens and broilers) fed with rations enriched with omega-3 polyunsaturated fatty acids (ω3FA) .Evaluating the effects of using in poultry feed by-products of vegetal origin.2.

P1 – Summarizing conclusions of studies from Tasks 1.Financial private contribution (%) at the project – 10. study of feasibility – month 2. experimental protocols – month 3.Number of enterprise participant – 1 participant – month 1-3 . liver. Task 1. CO.9% from the total budget of the phase – month 1-3 . feed raw materials and fodder. Deliverables (brief description and month of delivery) Results unlisted in annex 6 List of regulations in the domain of using phyto-additives in animals nutrition – month 1.4.3 and setting the list with by-products (titled as BYPROD) which will be characterized in phase 2. kidney and food of animal origin (meat. eggs).2 and 1. P3 –Development of a working methodology to determine components with antioxidant character in vegetal by-products and different types of feed.Value of the financial private contribution at the project – 9828 lei – month 1-3 facility of 24 PN-II-PT-PCCA-2011-3 . P4 – Working protocol to determine the antioxidant capacity from samples of brain. Analysis of by-products resulting from production activity of P1 that would arise from raw materials with antioxidant potential: CO. list of feed raw materials used to enrich poultry rations in ω3 FA.capacity in vegetal by-products. feed and biological samples : P2 – Working protocol to determine the antioxidant capacity in vegetal materials. P1 – Setting the terms of conditioning and modalities to include BYPROD in poultry forages. list of minimum 6 vegetal BYPROD proposed to be physically and chemically characterized from the perspective of the antioxidative potential and conditioning for embedding in feed– month 3 Results listed in annex 6 . Working protocol to determine the antioxidant effect of by-products on serum and poultry muscles.

weekly. .Valuing of nutritional quality of BYPROD . primary products of lipidic peroxidation (lipidic peroxides. Evaluation of antioxidant effect of BYPROD incorporated in feeds enriched in ω3FA: CO –From the 18 feeds prepared at the level of laboratory and stored at room temperature (15 0C).Development of a nutritional strategy to optimize feed recipes. P3 – Qualitative determination (identification) of active compounds as antioxidants. samples will be drawn out for comparative determination of fat degradation indexes (peroxide index. at the laboratory level.Phase no.Determining. conjugated dienes) and secondary products of lipidic peroxidation (compounds which react with 25 PN-II-PT-PCCA-2011-3 .Elaboration of the set of 9 feed recipes for hens and 9 feed recipes for broilers this way: FC recipe. for laying hens and broilers. on the same type and number of feed samples as CO.Determination of antioxidant capacity (AO) of the by-products and determination of microbiological load . respectively 9 feeds for chicken broilers (structured on phases of growth and finishing) Task 2. chemical composition and the potential as antioxidant agents for BYPROD proposed for study: P1 Conditioning of some experimental lots of BYPROD in order to characterize and include in batches of experimental feed CO – Determination of maintenance of the quality in time (during 1 month). Description of work (possibily broken down into tasks) and role of participants Task 2. FAOS recipe and 6 experimental feed recipes optimized against FC recipe (enriched in ω3FA ) by adding at each variant one from the 6 assortments of BYPROD.3. CO+P1 – Setting of a consensual level of BYPROD (chosen at the end of the preceding phase) inclusion in poultry feed. Fabrication. P2.1. enriched in ω3FA Involved partners Personmonths Start month End month CO 11 August 2012 P1 17 P2 4 P3 24 P4 0 Total 56 December 2012 Objectives . for a month period. of the 9 types of feeds for hens. P2 . CO. Kreiss reaction).2. enriched in ω3FA. Determination of the active compounds.Valuing of by-products (BYPROD) potential. Elaboration of feed recipes for hens/broilers and their preparation at the laboratory level: CO. Task 2. selected to be used as antioxidant agents in poultry feed. Phase title 2 Physical and chemical characterization of by-products (BYPROD) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed.

tiobarbituric acid -TBARS). broilers (FB_ω3FA) which will constitute control recipe in ”in vivo” conducted experiments. in feeds. 7. respectively.3. P3. audit certificate/2012. Task 2.2 and 2. classifying BYPROD. CO+P1+P2 summarizing results from tasks 2. broilers. Deliverables (brief description and month of delivery) Results unlisted in annex 6 Analysis bulletins – months 5. Will be selected the first two BYPROD (titled as BYPROD’S_1 respectively BYPROD’S_2) for embedding in feeds tested in experiments. Determinations concerning microbiological load.Qualitative determination concentration of active antioxidant components in those 18 feed samples collected by CO.Financial private contribution (%) at the project – 20.month 8 Results listed in annex 6 . Task 2. the first two will be selected by-products (titled as BYPROD’S) to be used as feed additives – month 8. progress report –month 8. in time.5 Annual meeting of consortium CO-presentation of web page. Selection of BYPROD which will be studied by ”in vivo” experimentation on hens and. 6.Number of enterprise participant – 1 participant – month 4-8 . classifying BYPROD according to their manifested antioxidant capacity. arranged based on antioxidant capacity (AO).4. respectively.2% from the total budget of the phase – month 4-8 . feed recipes enriched in ω3FA for hens (FL_ω3FA).Value of the financial private contribution at the project – 35363 lei – month 4-8 26 PN-II-PT-PCCA-2011-3 .

. brain. health status of hens.Ensuring of natural alternatives. diveded in four lots. kidney and liver samples. to the expensive synthetic compounds which are used at present for prevention of polyunsaturated fatty acids oxidation from laying hens feed .Phase no. partners in consortium Description of work (possibily broken down into tasks) and role of participants The experiments on animals will be hosted by CO. Organizing and conducting 3 experiments on laying hens: CO – will organize 3 experiments. nutritional and mycotoxicologic quality control of raw materials and feed. each of 8 weeks period. Phase title 3 Experimenting. will collect manure samples after 4. will follow bioproductive parameters (consumption. The experiments conducted on animals are conducted with the agreement of the commission for professional ethics of the institute. “in vivo”. egg production. scientifically founded and experimented.1. BYPROD’S_2. weight evolution).2. CO – Fabrication of 4 feed variants / experiment in pilot station from IBNA. starting with the second feeding week. of vegetal origin. eggs (18 eggs/lot) for physical and chemical analysis. to determine the effects of by-products (BYPROD’S) used as antioxidant feed additives in laying hens feed Involved partners Personmonths Start month End month CO 15 P1 21 P2 4 P3 30 P4 4 Total 74 January 2013 October 2013 Objectives . on eggs enriched in ω3FA . P3 – Determines the content of active antioxidant substances in fabricated feeds Task 3. respectively 8 feeding weeks. Task 3. carried out in their own experimental warehouses. 3.Diminishing of unwanted effects of polyunsaturated fatty acids oxidation from hens rations.Increasing the visibility of competence and expertise of research teams. Task 3. respectively the mixture 27 PN-II-PT-PCCA-2011-3 .Validation of some innovative solutions. 26 weeks age. of valuing some renewable natural by-products from local sources . Verifying the efficiency of BYPROD’S_1. Fabrication of feeds for laying hens and their quality control: P1 – Ensuring conditioned BYPROD’S (dried and ground) to include in feed. at hens slaughtering (6 hens/ lot) from the end of the experiment will collect serum.Increasing of antioxidant status of hens and evaluation of oxidative processes at the level of lipids and proteins from poultry meat and organs. Each experiment will use 120 hens. will collect biweekly. P2 –Achieves microbiological control and determination of antioxidant capacity from raw materials and feed fabricated in each experiment.

CO and P1.: Elaboration of documentation for patent application -feed recipe with the best results during the phase.5. 15. Task 3. glutathion reductase and of the reduced glutathion level from blood. yolk color intensity and frehness after 2.communication. Dissemination of results with relevance for scientific community and which are not subject of documentation for patenting: CO.Accepted for publish or published articles in the mainstream journals – month 14 . in group with P1. Haugh unit. cholesterol. determining of primary and secondary products of lipidic peroxidation from meat. P4 – determining the antioxidant capacity from samples of brain.6. of the data obtained in tests on preserving the quality of feed batches produced at the laboratory level. P3article within ISI quotation revue. evaluation of metmyoglobin and myoglobin level from poultry muscle.Registrated patent application – month 18 . organs and eggs. Financial private contribution (%) at the project – 16. 14. P2 . 12. analysis bulletinsmonths 9. Task 3.3% from the total budget of the phase – month 9-18. liver and egg yolk.Number of enterprise participant – 1 – month 9-18.4 Evaluating antioxidant status of hens and oxidative processes at the level of lipids and proteins P2 – determining the activity of antioxidant enzymes catalase. glutathion peroxidase. Task 3. progress report -month 18 Results listed in annex 6 . fatty acids profile. Task 3. determining the proteins solubility and electrophoretic profile of proteins from poultry muscle. classification of improved diets according to the obtained results in the 3 experiments – month 17. 10.(BYPROD’S_1+ BYPROD’S_2) used in feed rations of laying hens : CO will determine in egg samples (3 eggs/sample) physical parameters (weights. liver and kidneys. 13. peroxidase. Deliverables (brief description and month of delivery) Results unlisted in annex 6 Measuring bioproductive effects of experimented feeds -months 12. Value of the financial private contribution at the project – 663049 lei – month 9-18 . identification of occured problems and of modalities of overcoming and solving them. determines microbiological load from manure. 14. P4– elaboration of one article for journals with impact factor. 16. P2 – determining the antioxidant capacity from samples of serum. egg shell thickness. Evaluation of free radicals scavenging activity of egg yolk. vitamins and minerals) . pH.National and international mobilities – month 14 x persons 4 28 PN-II-PT-PCCA-2011-3 . 16.7 Annual meeting of consortium CO+P1+P2+P3+P4 Evaluation of project progress. respectively 4 storing weeks) and chemical parameters specific for yolk (fat.

sensorial parameters. aminoacids. brain.Determination of BYPROD’S effect on feeds and broilers meat stability . ash. will follow bioproductive parameters.2. Task 4. meat (breast and pulp chicken). fatty acids. Organization and development of 3 experiments on laying hens: CO – Will organize 3 experiments on broilers ongoing in own experimental warehouses. on 240 broilers 7 days of age. protein. 4. Fabrication of feeds for broilers and control of their quality: CO: Fabrication of the 4 variants of feeds/ experiment in the pilot station from CO-IBNA. Task 4. P2 –Accomplishes microbiological control and determination of antioxidant capacity from the raw materials and feeds fabricated in each experiment.Using some natural bioresources of local origin to diminish unwanted effects of fatty acids oxidation from broilers rations . Each experiment will be carried for 42 days. 3. Verification of efficiency of BYPROD’S_1.Phase no. nutritional and mycotoxicologic quality control of raw materials and feeds. Phase title Involved partners Personmonths Start month End month 4 Experimenting. P2 – activity of antioxidant 29 PN-II-PT-PCCA-2011-3 . will collect manure samples after 4. to determine the effects of by-products (BYPROD’S) used as antioxidant additives in broilers feed CO P1 P2 P3 P4 Total 13 21 4 18 4 60 November 2013 July 2014 Objectives .1.Obtaining safer. kidney and liver samples. The experiments conducted on animals are conducted with the agreement of the commission for professional ethics of the institute. divided in 4 lots. nutritional value (dry substance.Assuring processed BYPROD’s (dried and milled) in order to include them in feed. “in vivo”. P1. respectively 8 feeding weeks. cholesterol. at the broilers slaughtering (12 broilers/lot) at the end of the experiment will collect serum. respectively the mixture (BYPROD’S_1+ BYPROD’S_2) used in broilers feed rations: CO: will determine in meat samples (breast and pulp chicken) physical. BYPROD’S_2. P3 – Determines the content of active antioxidant substances in fabricated feeds. healthier and high quality food .Stimulation of partnerships between research units and private businesses in solving some complex problems Description of work (possibily broken down into tasks) and role of participants The experiments on animals will be hosted by CO. fat.Increasing antioxidant status of broilers and evaluation of oxidative processes at the level of lipids and proteins from chickens meat and organs . vitamins and minerals). health status of hens.

P2 + P4– dissemination at the university level by a student scientific society at the Faculty of Veterinary Medicine USAMV Bucuresti. unprotected by patent. liver and meat samples. according to their relevance for different categories of beneficiaries Deliverables (brief description and month of delivery) Results unlisted in annex 6 Optimized feed recipes . audit certificate /2013 month 20. articles and communications at professional events –months 24. analysis bulletins. Value of the financial private contribution at the project – 108048 lei – month 19-27 . evaluation of the level of metmyogobin and myoglobin from poultry muscles.National and international mobilities – month 24. Results listed in annex 6 . experimental observations and conclusions resulting in microtests –month 26. 26 . liver and kidney. solubility of proteins and electrophoretic profile of proteins from chicken musculature.seminars at the Faculty of Pharmacy from UMF Bucuresti Task 4.Accepted for publish or published articles in the mainstream journals – month 24. 26 x persons 4 30 PN-II-PT-PCCA-2011-3 . classification of the recipes based on bioproductive effects and maintaining the feed quality and meat quality – month 26. P3.enzymes catalase.month 27.4. P4 – determination of antioxidant capacity from brain. Task 4. by participation at exhibitions organized by the patronage from food industry. determines microbiological load from manure.5. 1 seminar at the Faculty of Pharmacy – month 24 . Financial private contribution (%) at the project – 16. 25.Number of enterprise participant – 1 – month 19-27. peroxidase. P1: dissemination of the results from the previous phase. experimental report regarding scientific data. 25. report on the project progress . glutathion reductase and of the level of reduced glutathion will be determined from blood. Dissemination of the results on used working methodologies: CO: elaboration of articles together with P1. scientific society at the Faculty of Veterinary Medicine – month 23.month 19. 26.29% from the total budget of the phase – month 19-27. data base– months 12-17. P3. P2. Annual meeting of consortium CO+P1+P2+P3+P4 Analysis of the obtained results and of valuing modalities. 25. glutathion peroxidase.

Task 5.3 Dissemination at the level of the business field and government organizations CO: disseminates project results on the project web page. Elaboration of the documentation for patent application of a new feed recipe. Deliverables (brief description and month of delivery) Results unlisted in annex 6 Business plan -month 32.Prevention of accumulation of by-products resulted from food industry and food supplements industry Description of work (possibily broken down into tasks) and role of participants Task 5. roundtable – months 30. through presentation at the annual meetings with farmers organized in spring at IBNA.1. Lumea Satului. workshop. of valuing byproducts from food industry in animal nutrition . AGRALIM.Phase no. presenting results at symposiums in the field. Avicultorul.4. 32. P3 – article in ISI journal. of the degree of implementation and elaboration of the final report. P1: organizes a workshop.2. Economic analysis of the impact of project at P1 P1 – Elaborating a business plan Task 5. founded and scientifically experimented.month 31. enriched in omega-3 fatty acids. article in ISI journal together with P2. Poster at the national exhibitions INDAGRA. CO+P1 elaborates a brochure to promote the results of the project at the level of the business field and government organizations. Task 5. 31. brochure for information and awareness of consumers. AGRARIA. Dissemination of the results in the scientific field: CO – presenting the results at the international symposium IBNA. P1 – Elaboration of documentation Task 5. participation at national exhibitions addressed to the 31 PN-II-PT-PCCA-2011-3 . P4 article in ISI journal. Phase title Involved partners Personmonths Start month End month 5 Dissemination and exploiting of the obtained results CO 12 P1 12 P2 2 P3 6 P4 2 Total 34 August 2014 February 2015 Objectives -Summarizing and correlation of obtained results in all the phases of the project -Dissemination of the results which are not subject to patenting -Dissemination of the results at the level of national exhibitions dedicated to the economic field -Analysis of the economic results obtained by the economic agent –business plan -Integration of some innovative solutions. publish 3 articles in the journals The Farmer. Final meeting of consortium: Appreciation of the obtained results. for broilers: CO.5.

Financial private contribution (%) at the project – 16. article etc Phase no. 30 .. 5 application application Phase 5 – month 29 according to Annex 6 – results indicators of the Programme (patent.Number of enterprise participant – 1 – month 28-34 . audit certificate/2014. n) Phase 1 – month 1-3 Number of enterprise Phase 2 – month 4-8 P1 .National and international mobilities – month 29. audit on the project-months 34 Results listed in annex 6 .Accepted for publish or published articles in the mainstream journals – month 29. technology.Registrated patent application – month 29 .42% from the total budget of the phase – month 28-34 . 1 2 3 4 5 6 * of Phase delivery date (1 .. Type Deliverable* 32 PN-II-PT-PCCA-2011-3 .Hofigal participant – Phase 3 – month 9-18 1-5 1 partcipant Phase 4 – month 19-27 Phase 5 – month 28-34 Phase 1 – month 1-3 Financial private Phase 2 – month 4-8 Financial private contribution (%) at Phase 3 – month 9-18 1-5 contribution (%) the project Phase 4 – month 19-27 Phase 5 – month 28-34 Phase 1 – month 1-3 Value of the financial Phase 2 – month 4-8 Value of the financial private contribution Phase 3 – month 9-18 1-5 private contribution at the project Phase 4 – month 19-27 Phase 5 – month 28-34 Accepted for publish Accepted for publish or published articles Phase 3 – month 14 or published articles in in the main Phase 4 – month 24-26 3-5 the main international international Phase 5 – month 29-30 scientific flow scientific flow National and National and Phase 3 – month 14 international international Phase 4 – month 24-26 3-5 mobilities mobilities Phase 5 – month 29-30 Registrated patent Registrated patent Phase 3 – month 18 3.business field. final report.Value of the financial private contribution at the project – 39291 lei – month 28-34 . 30 x persons 4 Table: Deliverables List Deliverable Deliverable Name No.months 32.

reviewing to verify consistency and submitting reports and other deliverables (including financial statements and related certifications) to the Contracting Authority. Management structure and procedures The Project Coordinator will act as overall leader of the project. Management and distribution of financial support amongst the partners. The Management Committee will be responsible for all strategic and administrative decisions of the project. which will be responsible for daily management and administration of the project work. and will be responsible of the financial administration and Project meeting administration. The Management Committee makes decisions upon consensus.1. Coordination of knowledge management and other innovation-related activities. implementation and oversight an appropriate Intellectual Property Strategy. Agreement. To assist the coordinator in approving publication of scientific articles and presentation of results at national and international meetings. however. Active involvement in the promotion of the project. ensuring the transfer of knowledge to the end users. in the unlikely event of no agreement being reached. To agree the scientific details of the work program. Obtaining audit certificates by each of the contractors. each member will have one vote for decision making. Overseeing the efficient distribution of funds to the consortium members as per the agreed budget. Analyzing the performance indicators. Submitting financial statements and receiving in trust for the consortium all payments from the Contracting Authority and distributing them among the contractors according to their decisions. The Project Coordinator will oversee The Management Committee (consisting in Project Manager and Project Responsible of the Parties) and The Administrative and Financial Service (department of Coordinator Institution).Implementation 2. Project Coordinator main duties will include: Chairing the Management Committee. 33 PN-II-PT-PCCA-2011-3 . Transmitting documents and information connected with the Project to any other Parties concerned. being the intermediary between the Parties and the Contracting Authority and will perform all tasks assigned to it as described in this Consortium Agreement. The committee will meet at least twice a year throughout the project. Monitoring compliance by the Parties with their obligations. Collecting. The Administrative and Financial Service will consolidate the project reports provided by Project Responsible of the Parties and the Project Coordinator. will be composed by the Project Manager and the Project Responsible of the Parties. taking into account the evolution of the context in which the project is carried out. Chaired by the Project Coordinator. A meeting of this committee has already taken place and was responsible for the drafting of this application. The responsibilities of the Management Committee will be: The overall direction of all activities. with the coordinator having the casting vote.

is the National Institute in the field of animal science in Romania.3 Nutrition and Health). IBNA has one of the best research infrastructures in the whole Romanian agriculture research: facilities for animal nutrition physiology. experimental farms (main farm animal species). The team is currently involved in several projects relevant for this project proposal activities and has experience in FP/6/7 and Eureka projects.Contracting Authority CO Administrative financial service Management Committee P2 Project development Multidisciplinary studies Fundamental research Experimental reasearch Identification and characterization of sub-products which will be included as antioxidant feed additives P1 CO P3 In vivo experiments. new functional food on Romanian market (CEEX national programme/ 22/2005). Some recent projects include: Eggshells processing and eggs with functional food properties production (EUREKA Technological Area. In 2011. having direct responsabilities concerning: the development of nutritional strategies. the institute has a good logistics for knowledge transfer. 8. The determination of sub-products’ effects Dissemination of the obtained results Scientific papers.2. Also. arable land & facilities for producing experimental forages. the institute has 30 projects (25 national and 5 international ones) from which 17 as coordinator. la puii broiler (AGRAL national programme 132/ 2002) Role IBNA is project’s coordinator. patents P4 2. The Institute is involved in all phases of the project. Optimizarea structurii de nutreţ combinat sub raport vitamina E / seleniu / acizi graşi în vederea îmbunătăţirii calităţii carcaselor. Optimization of nutritional factors to obtain enriched egg consumption in polyunsaturated ω: 3 fatty acids . IBNA has complex researchdevelopment activities in the field of the biology and nutrition of all species and categories of farm animals (from research to extension). an experimental feed mill. Individual participants Coordinator of the project (CO): NATIONAL RESEARCH & DEVELOPMENT INSTITUTE FOR ANIMAL BIOLOGY AND NUTRITION (IBNA BALOTESTI). the study of the new 34 PN-II-PT-PCCA-2011-3 .

56 years. She is the project manager. Research Director in Hofigal. Dr Viorica Tamas (PhD). provides and produces finished products at the pilot station. 32 years. 56 years. dietary supplements. researcher for the improvement of existing technologies. 29 years.(USAMVB) . major participation in other ten projects) and work experience in FP6. Valentin Razvan Nicorescu (PhD). USAMVB associates educational activities with research activities in the following fields: processing of animal productions. Tatiana Panaite (PhD). with antioxidant potential.C. nutritional solutions for functional foods. USAMVB is an approved state university in agriculture field. Faculty of veterinary medicine. Key persons: Natalita Bordei. and phytotherapeutic galenical products in Romania. Partener 2 (P2): UNIVERSITY OF AGRONOMICAL SCIENCES AND VETERINARY MEDICINE BUCHAREST. veterinarian. Key persons: Rodica Diana Criste (PhD in animal science). gemoderivative. is a senior researcher and Professor in Biochemistry Department. In order to attain the quality objectives. eggs and poultry manure. 34 years. head of Department of Chemistry and Physiology of Nutrition in IBNA Balotesti. on lipid peroxidation of PUFA from poultry feed. Processed by-products will be subject to quality control and analysis using modern means. Andreea Cozea (PhD). 33 years. responsability of project management within IBNA. author and co-author of over 65 patents and inventor certificates. development of nutritional solutions to limit the polluting risks. coordinates the technical.A (HOFIGAL) is one of the famous brand names when it comes to homeopathic. senior resercher (I). veterinarian and biologist. EUREKA projects. achieves microbiological control in feed. senior researcher (III). chemist. the determination of the nutritional quality of the by-products. will provide the necessary quantities of plant byproducts during all stages of the project. cosmetics. Her scientific fields of interest are: poultry & pig nutrition. Partner 1 (P1) : S. She has experience in coordinating national research projects (8 projects as project manager. Untea Arabela (PhD student). chemist. and teas. works in the field of those vegetal extracts that can be 35 PN-II-PT-PCCA-2011-3 . administrative and financial activities of the project. Key persons: Camelia Puia Papuc (PhD). Role: USAMVB will participate in all phases: evaluating the effects of using in poultry feed by-products of vegetal origin. Role: HOFIGAL SA. made in the form of pharmaceutical products. eggs and broiler meat that were studied. feed. Mircea (PhD student). specialist in Eugenia biotechnology . 32 years. FP7. Researches in the last 5 years are focused on antioxidant activity of plant polyphenols. 55 years. quality check of feeds and animal products. USAMVB. senior researcher. effective for this purpose. spectrometric determinations.HOFIGAL EXPORT – IMPORT S.solutions by organizing experiments on broilers and laying hens which are developed in their halls. senior researcher (III) on poultry nutrition physiology. food safety and security.

(Optimization of the nutritional factors for the production of ω : 3 polyunsaturated fatty acidsenriched egg. UMF is a higher-education institution. Faculty of Pharmacy . The team of the present project contains 6 academics involved in several projects. 41 years. UB is one of the biggest university in Romania. which is currently running. in medical domain and related fields. Dr Valentina Popa (PhD). Partener 4 (P4) BUCHAREST UNIVERSITY. 39 years. The team designated for developing this project was involved in several national projects recently finalized. 36 PN-II-PT-PCCA-2011-3 . senior lecturer and the head of the Department of Pharmacognosy Phytochemistry Phytotherapy. 2. senior lecturer at the Department of Pharmacognosy Phytochemistry Phytotherapy. Their very fruitful collaboration from these projects is one of the reasons why these partners got together again for this project proposal. Consortium as a whole The project is vast and ambitious and its accomplishment is guaranteed by the professional and technological capacity of the consortium. Partener 3 (P3) UNIVERSITY OF MEDICINE AND PHARMACY „CAROL DAVILA“Bucharest. P1 and P2 have already been partners before. scientific explanation (based of chemical composition) of pharmacologic activity of herbal products. as new functional food for the Romanian market). Key persons: Dr Andrei Florin Danet (PhD) (65 years) analytical chemistry scientist. 43 years. The project coordinator (CO). in FITOMIN national project (The reduction of the level of mineral trace elements in pig manure by the replacement of chemical premixes with phytogenic additives and enzymatic preparations. senior researcher in analytical biochemistry. Faculty of Chemistry . way of promoting the sustainable agriculture).(UB). concerning the following: introduction/evaluation of new herbal raw materials in therapy. ensures project promotion and dissemination of the project’ results. it will contribute to different dissemination activity. member in 8 national projects. 45 years. biochemist. and with P2 in SUPLIBOR national project (Transdisciplinary research concerning on the essentiality and the additional levels of boron in broiler meat and piglets) project. Role: UB will perform determination of antioxidant capacity of the brain and liver poultry samples. member in 6 national projects. CO has also been partner with P1 in OMEGANUTRIOSAN national project. Ligia Elena Duţu (PhD). Key persons: Cerasela Elena Gîrd (PhD).(UMF). 20 years work experience in the field of pharmaceutic education. classified as a university with advanced research activity and education. Maria Lidia Popescu (PhD). He published more than 100 papers in different branches of analytical chemistry. Role: UMF is involved in activities which aims: qualitative chemical analysis for vegetal raw materials and feed in order to identify antioxidants. yolk egg samples.used in veterinary medicine.3. He was involved in 6 European projects.

Phase 1 Task CO P1 P2 P3 P4 Task CO P1 P2 P3 P4 1. The table below shows the interconnection between partners throughout the project.6 3.2 1. environmentally controlled experimental houses fitted with new (2008.3.1 1. having responsibilities particularly in the tasks concerning the by-products which resulted from the processing of the plants grown in its own greenhouses. P4 – UB consists of teaching staff from the Faculty of Chemistry.3 4. laboratories for chemical and biochemical investigations (some of which are ISO 17025 accredited).CO – IBNA.3 5. This department has the proper analytical equipment to assay the by-products (processed by P1) and the active antioxidants from the compound feeds (manufactured by CO). Hofigal is involved in all stages. 3. properly endowed with research and analytical equipment and instruments. The Biochemistry Department of USAMVB has all the specific and necessary equipment and instruments to evaluate the effects of using in poultry feed by-products of vegetal origin. most of whom are PhD or PhD students. with antioxidant potential.4 and 5.4                                 37 PN-II-PT-PCCA-2011-3 .2 2. eggs and poultry droppings. has all the adequate infrastructure needed for this kind of projects: pilot mill for compound feeds manufacture.3 1.4 3. P3 – UMF has a team consisting of teaching staff from the Department of Pharmacognosy Phytochemistry Phytotherapy of UMF.2.4                    5. P2 – USAMVB has a team of teaching staff involved in research focused on the antioxidant activity of plant polyphenols.2 5. is involved in all phases of the project and in all its tasks.5 Phase 5 5.2 3. except 1. All the partners participate in the dissemination activities and in the consortium meetings. 5 Phase 3 3. The working team is multidisciplinary and it consists of specialists in animal nutrition. and these dried and grounded samples will be send it to CO which will use it in the formulation of the compound feeds for poultry.5                      Phase 4 4.1 2. P2 and P3.2 4. 5 3.3 3.1 3.3 2. it also performs the microbiological control in feed.7         4. on lipid peroxidation of PUFA from poultry feed.4 Phase 2 2.1 4.1 5.4 2. who have the necessary expertise to determine the antioxidant capacity in the biological samples collected by CO during the trials on layers and broilers. P1-Hofigal has a strong research department. 2009) cage systems for poultry and good logistics for knowledge transfer. These by-products are transferred as analytical samples to CO.

4. CO performs activities which involves: identification and characterization of vegetable byproducts. thermostat. centrifuge. laboratory fridges.To achieve multidisciplinary studies and dissemination of results. 2 HPLC systems for vitamins and amino acids determinations. laboratory mill. P2 will involve the project team consisting of 6 researchers and will use the existent equipment: ovens. 3 workers. P2 . pH-meter.To achieve multidisciplinary studies and dissemination of results. vertical freezer. in the pilot station for compound feeds.To achieve multidisciplinary studies and dissemination of results. 3. UV lamps.To achieve multidisciplinary studies framed in the first phase of the project and dissemination of results. system of video-documentation in gel. nutritional and mycotoxicological quality control of raw material. automatic potentiometric titrator. These will be manufactured within the coordinating institution. egg shell analyzer. Resources to be committed CO . To achieve the objectives. electrophoresis device in vertical gel. P2 will carry out activities involving the determination of by-products and feed antioxidant capacity used in experiments and determination of microbiological load. microcentrifuge. egg shell thickness gauge. 3GB DDR3. and it will be used experimental and digestibility cages for poultry. 3 and 4. automatic soxhlet extractor. P3 . FOSS systems. 3 and 4. water bath. P2 will involve 3 key persons and the existent computerized systems. P1 must acquired PHOTOCHEM analyzer within the project and the interpretation of data analyzer will be made by purchasing a system desktop Serioux Explorer V8 Intel Pentium Dual Core E5700 3 GHz. rotary evaporator. In phases 2. pH-meter. coling heating thermostat. For carrying out these activities will be involved 10 researchers. P1 will realize the conditioning and the assurance of the experimental groups of BYPROD'S. To conduct these activities all 7 key persons will contribute. analytical balances. In phases 2. rotary-evaporator. In phases 2. CO will use: 6 key persons and computerized systems. turbidimeter. spectrophotometer. In phases 3 and 4 will be produced batches feed necessary for laying hens and poultry experiments. determination in egg and meat samples of physical and chemical parameters. chemiluminometer. P1 . electrophoresis device in horizontal gel. P1 will involve 7 key persons and the existent computerized systems. It will use existing equipment in the chemistry laboratory: GC for fatty acids determinations. 500GB 32MB. homogenizer. 2 technicians. analytic balance. deepfreezer. 4 laboratory assistants. complete ELISA line. calcination oven. nise. magnetic agitators. athomic absorption spectrophotometer. P2 will involve 6 38 PN-II-PT-PCCA-2011-3 . blender.4. 4 technicians. freezers.2. For rapid analysis of antioxidants and free radicals. centrifuge. spectrophotometer UV-Vis-NIR.There will be involved 4.

For phytochemical analysis P3 needs to purchase: hot air oven with accessories. fluorimeter. In phases 3 and 4. In phases 2. autoclaves hood with laminary flow. pharmaceutical sieves with accessories. which will be characterized in phase 2.5. from Hofigal. vacuum-pump. moisture balance.In this phase is aimed to create the technical and logistic framework. liver and meat samples. electric sand-bath. centrifuges. conditioned by P1. Also it will be setting the list with 6 by-products (titled as BYPROD). electronic balance. spectrofotometer UV-VIS. ultrasonic bath with accessories. glass filter holder assembly with accessories. 2. enriched in ω3FA From the 6 BYPRODs chosen at the end of the first phase.key persons and the existent computerized. electrochemical equipment for potentiometric. antioxidant capacity. we plan to have applied interdisciplinary research activities during 5 phases: Phase 1 Multidisciplinary studies regarding methodologies. organs and eggs. A team of 2 researchers will collaborate and will use the equipment: spectrophotometers with optical fibers. P4 will determine antioxidant capacity of brain. P3 will involve a team of 6 persons and will use existing lab equipment: chromatographic system. supercritical fluid chromatography and extraction system. active 39 PN-II-PT-PCCA-2011-3 . will be made determinations concerning their nutritional value. TLC plate heater with accessories. binocular microscop with digital photo camera. setting methodologies and modalities to determine antioxidant effect of by-products on feed. P4 will involve 2 key persons and the existent computerized systems. Phase 2 Physical and chemical characterization of by-products (BYPROD) of vegetal origin and valuing their efficiency as feed additives with antioxidant role in laying hens and broilers feed. a chemiluminometer. analytical balance. To achieve the experimental determinations and to elaborate the conslusions. where will be carried out the project activities concerning: nutritional integrative solutions of optimizing feed recipes for laying hens and broilers by valuing some natural vegetal by-products of local source from the perspective of diminishing unwanted effects of oxidation of fatty acids from poultry rations. rotary-evaporator. Deliverables (listed in annex 6): the value of the private (HOFIGAL) financial contribution within the project is 9828 lei. pH-meter. Methodology and associated work plan: In order to accomplish these general objectives. amperometric and voltammetric determinations. poultry organisms and evaluating oxidative processes at the level of lipids and proteins from poultry meat. P4 -To achieve multidisciplinary studies and dissemination of results. flask heaters with accessories. analytic and precision balance. 3 and 4. working techniques and economic analyzes of evaluation of effects of using some by-products of vegetal origin as antioxidants in the feed of laying hens and broiler chickens. laboratory oven. P2 will determine active compounds as antioxidants in vegetal by-products and will produce the feed. electric water-bath.

based on the feed recipes. At the end of the phase will take place a first consortium meeting. At the end of each experiment will be slaughtered 6 hens/lot and will be collected serum. with the best results. for each experiment. to determine the effects of by-products (BYPROD’S) used as antioxidant feed additives in laying hens feed. (iii) 6 feeds structured as FC but in which will be embedded. distributed in 4 lots/ each experiment). glutathion peroxidase. hens and broilers. at the level of laboratory batch: (i) a standard feed. glutathion reductase and of the level of reduced glutathion will be determined from blood. Will be collected also manure samples which will be microbiologically analyzed. brain and kidney samples. liver and kidney. After summarizing the results obtained in the three experiments will be elaborated documentation for patenting the feed recipe with the most efficient action in terms of eggs enrichment in ω3FA under the conditions of preventing emergence of oxidation reactions in feed 40 PN-II-PT-PCCA-2011-3 . in this phase will be conducted 3 experiments on laying hens (120 hens. During the experiments will be followed bioproductive parameters and physical and chemical parameters of reference for assessing eggs quality. On the basis of the obtained results will be able to select two by-products (titled as BYPROD’S_1 respectively BYPROD’S_2). at a level of exploration. (ii) two types of feed enriched in ω3FA (by a raw oleaginous material which will be decided depending on the disponibility on the local feed market) from which one type of feed without antioxidant (titled as FC) and a type of feed (titled as FAOS) with one synthetic antioxidant (vitamin E) . BYPROD compounds thus characterized will be taken into account for elaboration of a set of 18 feed recipes (9 for laying hens and 9 for broilers). peroxidase. a mixture between BYPROD’S_1 + BYPROD’S_2 (experiment 3) added to the rations. national and international mobilities( month 14 x persons 4) Phase 3 Experimenting. Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 663049 lei. will be made comparative determinations regarding maintaining the quality in time (during 1 month). will be prepared. From the 18 variants of feeds fabricated at the level of a laboratory. For each. “in vivo”. 4 variants of feeds differentiated as follows: FL_ω3FA (with ω3FA but without BYPROD’S ) and 3 experimental variants which are differentiated from variant FL_ω3FA by the levels of BYPROD’S_1 (experiment 1). liver. one of the 6 BYPROD studied previously. From the samples of eggs and organs will be determined primary and secondary lipidic peroxidation products. Will be fabricated. registrated patent application .compounds as antioxidants. for ”in vivo” experimentations in the next 2 phases. To validate innovative solutions of using BYPROD’S as antioxidant feed additives efficient in laying hens rations enriched in ω3FA (FL_ω3FA). BYPROD’S_2 (experiment 2). Activity of the antioxidant enzymes catalase. 2 articles in the mainstream journals . and the concentration of the active antioxidant components. microbiological and mycotoxicologic load.

BYPROD’S_2 (experiment 2). Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 663049 lei. problems which occur and solving solutions. 2 articles in the mainstream journals. national and international mobilities( month 24 x persons 4) Phase 5 is for dissemination and exploiting of the obtained results. At the end of each experiment will be slaughtered 12 chickens/lot and will be collected meat (breast and calf). brain and kidney samples. During the phase will be conducted 3 experiments. 2 articles in the mainstream journals. Will be collected also manure samples which will be microbiologically analyzed. The 4th phase has a development algorithm similar with that from the first part of preceding phase. Will be also done: determinations on the proteins solubility and electrophoretic profile of proteins from chicken muscles. registrated patent application national and international mobilities( month 29. In the case of recipes for chickens.3 x persons 4) 41 PN-II-PT-PCCA-2011-3 . for each experiment.and egg by using a BYPROD’S. 2 articles in the mainstream journals . The level of BYPROD’S added will not be differentiated between the growing phases. liver. 4 variants of feeds: variant fabricated by recipe FB_ω3FA (without BYPROD’S ) and 3 experimental variants which are differentiated from variant FB_ω3FA by the level of BYPROD’S_1 (experiment 1). Each experiment will be carried for 42 days. Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 108048 lei. At the end of the phase will be conducted the consortium meeting which will analyze obtained results. evaluation of the metmyogobin and myoglobin level from the poultry musculature. serum. Will be fabricated. During experiments will be followed consumptions and gains. By the activities of this phase is followed the validation of innovative solutions of using BYPROD’S as efficient feed antioxidant additives in broilers rations enriched in ω3FA (ratia FB_ω3FA). the basic structure will be established according to the growing phases. 1registrated patent application . From the meat and organs samples will be determined primary and secondary products of lipidic peroxidation. in terms of chicken meat enrichment with ω3FA in conditions of prevention of the occurence of oxidation reactions of polyunsaturated fatty acids. on 240 chickens 1 days of age. national and international mobilities( month 14 x persons 4) Phase 4 Experimenting “in vivo”. After summarizing the obtained results in the three experiments will be elaborated patenting documentation of the feed recipe with the most efficient action. to determine the effects of by-products (BYPROD’S) used as antioxidant additives in broilers feed. both in feeds and in meat. divided in 4 lots. Deliverables: the value of the private (HOFIGAL) financial contribution within the project is 39291 lei. stage of project progress. mixtures of BYPROD’S_1 + BYPROD’S_2 (experiment 3) which are added to the rations.

3 A IV. Trim I .4 A III.4 A II. III Trim .1 A IV. IV Trim.2 A II.4 A II. I IV 2012 Etapa I 2012 Etapa II 2013 Etapa III 2013 / 2014 Etapa IV 2014 / 2015 Etapa V Key persons list (their CVs are uploaded on the web platform) Name surname* Coordinator (CO) Criste Rodica Diana and Scientific title CS 1 Phase Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Untea Arabela CS 3 Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Olteanu Margareta CS 2 Phase 1 Phase 2 Phase 3 Person-month 1 2 3 3 2 1 2 3 2 2 1 2 3 42 PN-II-PT-PCCA-2011-3 . I 2013 Trim .2014 Trim. Trim.3 A III.Gantt Diagrama Etapa Categ.3 A II.5 A V.1 A I.4 A IV.6 A III. II Trim.7 A IV.5 Trim. III Trim. Activ.2 A III. A I.2 A I.3 A I.2 A IV.4 A V. II 2012 Trim.5 A III.3 A V.1 A II.5 A III.2 A V. II Trim.1 A III. IV 2013 .1 A V. III 2014 – 2015 Trim.

Phase 4 Phase 5 Mircea Eugenia AC Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Panaite Tatiana CS 3 Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Ropota Mariana CS 2 Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 1 Bordei Natalita Chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Tamas Viorica PhD chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Neagu Mihaela Phd biochem Phase 1 Phase 2 2 2 1 2 2 2 2 1 2 2 2 2 1 1 2 2 2 3 3 3 3 2 3 3 3 3 2 2 3 3 43 PN-II-PT-PCCA-2011-3 .

Professor Phase 2 Phase 3 1 1 3 2 2 2 3 3 2 2 2 3 3 2 2 2 3 3 1 2 2 3 3 1 1 2 2 2 1 44 PN-II-PT-PCCA-2011-3 .Phase 3 Phase 4 Phase 5 Cozea Andreea Phd Bioteh Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Traistaru Alina Gina Phd Chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Badea Elvira PhD Biol Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Carabela Viorica Eng Chem Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 2 Papuc Camelia Puia PhD. Professor Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Crivineanu Maria PhD.

Lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 3 Gird Cerasela Elena PhD.Phase 4 Nicorescu Valentin Razvan PhD. lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Popescu Maria Lidia PhD. lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Dutu Ligia Elena PhD. assistant Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Nencu Ioana PhD student. lecturer Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Iordache Alina Titina PhD student. assistant Phase 1 Phase 2 Phase 3 1 1 1 1 1 1 2 4 5 3 1 2 4 5 3 1 2 4 5 3 1 2 4 5 3 1 2 4 5 45 PN-II-PT-PCCA-2011-3 .

Analytical balances Sartorius and Kern.S. P1 . EF-2Bulk ELECTROLAB TESTER ASA Technology. Whirpool COLD LADA 2003.5 0 2 2 1 Available research infrastructure CO .C. Rotary-evaporator Buchi. . grains and seeds with knives and hammers IMUC. Calcination oven.INCDBNA-Balotesti . Deepfreezer ULUF 450. Electric driers S. Laboratory mill. Gas chromatographer for fatty acids determinations PerkinElmer (USA). Temperature adjustable oven VENTICELL BMT.A. Nise. Cardinal Service. Professor Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Valentina Popa PhD Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Total 261 3 1 2 4 5 3 1 0. assistant Phase 1 Phase 2 Phase 3 Phase 4 Phase 5 Partner 4 Andrei Danet PhD. FOSS Soxtec 2055. Egg shell analyzer. FOSS Fibertec 2010.5 0 2 2 1 0. OIL 46 PN-II-PT-PCCA-2011-3 . Homogenizer. Digestibility cages for poultry. Pilot station for compound feeds. Freezer DKF 350. OIL EXTRACTION INOX. Ecocell oven 222.. FOSS Kjeltec 2300.Phase 4 Phase 5 Costea Teodora PhD student.C. Mill Plants. HPLC Termo Electron. LIQUID. Egg shell thickness gauge. Centrifuge. HPLC PerkinElmer (USA). Spectrofluorimeter.Experimental house. Hofigal Export-Import S. Athomic absorption spectrophotometer. HARDNESS TESTER Logan HDT-300 ASA Technology.

Pneumatic conveying plants TOCATOR Plant. Rotary evaporator Heidolph. Binocular microscop with digital photo camera Zeiss. Spectrofotometer UV-VIS V-530 Jasco. Laboratory oven Ecocell. amperometric and voltammetric determinations. UV lamps. Analytic balance Precisa. Water bath GFL 1092. Vacuum-pump Fischer. Turbidimeter Cole Parmer. Autoclaves hood with laminary flow. Automatic potentiometric titrator Titro Line easy. Electrochemical equipment for potentiometric. Fluorimeter Able Jasco.STORAGE STAINLESS UPRUC. Laboratory fridges Nitech. SpectrophotometerJena. Analytic balance KERN. DRY HOT AIR TRAYS-Valcea Ramnicul P2 . pH-meter. Centrifuges 47 PN-II-PT-PCCA-2011-3 . Microcentrifuge Beckman. Chemiluminometer Turner Biosystems (USA).USAMV – Facultatea de Medicina Veterinara Bucuresti: Ovens Nitech. Thermostat Nitech. Electrophoresis device in horizontal gel Consort. Blender Waring. Supercritical fluid chromatography and extraction system (SFC / SFE) Jasco P4 – UB – Facultatea de Chimie: Spectrophotometers Ocean Optics (USA) with optical fibers. Electrophoresis device in vertical gel Consort. Moisture balance Kern. System of video-documentation in gel Vilber Lourmat. Palm Sens (Holland). Rotary-evaporator Buchi. Chemiluminometer – Berthold FB12. Analytical balance. Electric sandbath. PH-meter – Sigma. Electric water-bath Raypa. Complete ELISA line. Spectrophotometer UV-VisNIR V670.UMF „Carol Davila” – Facultatea de Farmacie: Chromatographic system Reprostar 3. Coling Heating Thermostat Biosan CH 100 P3 . Electronic balance Shimadzu.

42 Budget breakdown by category of expenses Budget breakdown / destination (lei)1 Personnel costs Coordinator (CO) Partner 1 Public Budget Private cofinancing Public Budget Private cofinancing Partner 2 Public Budget Private cofinancing Partner 3 Public Budget Private cofinancing 1 Logistics Equipments 0 Travel Materials 110400 Subcontracting 10000 10000 Indirect costs 130400 Total 391200 652000 125000 60000 60000 167460 180579 0 100000 36000 25100 75000 30000 60740 24000 558200 300579 116300 0 15000 16200 32348 30000 30692 0 6000 17260 116300 According to Chapter 8 – Budget 48 PN-II-PT-PCCA-2011-3 . Budget breakdown by year (lei) Public Budget Private cofinancing Total 2012 2013 2014 2015 Total 2012 2013 2014 2015 Total 2012 2013 2014 2015 Total Priv ate cof % 16.42 (CO) P1 P2 P3 P4 Total 98012 83913 17483 17483 2850 219741 234380 200660 41807 41807 36346 555000 234380 200660 41807 41807 36605 555259 85228 72967 15203 15203 11399 200000 652000 558200 116300 116300 87200 1530000 45191 108049 108048 39291 300579 98012 129104 17483 17483 2850 249111 234380 308709 41807 41807 36346 625231 234380 308708 41807 41807 36605 662805 85228 112258 15203 15203 11399 262853 652000 858779 116300 116300 87200 1830579 16.Table 7.

500 mL and 1000 mL flasks with accesories Pharmaceutical sieves with accesories TLC plate heater with accesories Ultrasonic bath with accesories Glass filter holder assembly with accesories . mouse. Operating system: MacOS X Lion Justification . HDD 500GB/7200rpm. IR receiver.Proces simulation Tehnical repport edditing . DDR3 1333Hz. WI-FI.GDDR5.Complet system for determining antioxidants which are soluble in water and lipids. 2. Full measurement of antioxidant capacity from plant materials.Partner 4 Public Budget Private cofinancing 61760 4000 4000 17440 87200 Total 730308 378039 306192 85000 65000 266040 1830579 Justification of purchasing major pieces of equipment Equipment name and characteristics Partner 1 -Computer Desktop all in one.5’’. Measurement of whole antioxidant capacity ( the sum parameter) the most diverse and compelx mixtures of plants. Memory 4GB. KB wireless. SD.5GHz. . 21. IntelCore i5. 2.PHOTOCHEM (Analytik Jena Romania srl) Rapid analysis of antioxidants and free radicals with PCL method (Fotochem luminiscent) Complete system for determining antioxidants The first tool that allow a unique determination of both water and lipids soluble antioxidants. Partner 3 -Hot air oven with accesories Flask heaters. bluetooth. 250 mL.1+EDR. for 100 mL. video AMD Radeon HD 6750M/512MB.Phytochemical analysis 49 PN-II-PT-PCCA-2011-3 .

master students and students involved in the project. Of the 24 key people. 3. Added value of the project results at National.Expected impact 1. Further consumer’s expectations involve the origin and the image of the product.5%) and 5 are PhD students. 9 are young people under 35 years (37. by promoting a healthy lifestyle and with direct implications on the healthcare system. Impact on the development and promotion of young people –  Professional training of young PhD students. ecology. there is strong need for specific information and communication activities to consumers in this regard.1. health.  Direct and indirect reduce of costs associated with cardiovascular disease. Environmental impact • The project does not prejudice the environmental quality on the contrary. generate market benefits for producers of eggs (functional foods).  Provide support information for farmers and employers of the feed and food industry. Social Impact  Because of the limited consumer awareness and limited health effects awareness of new functional ingredients developed. hygiene and taste. The people want and have the right to know what they eat. Dissemination and/or exploitation of project results. having a lower production cost.2. European and International level Economic Impact  The project will generate economic benefits to the co-financing economic agent due to the using of a vegetal source with antioxidant potential as an alternative to expensive synthetic products. as well as animal welfare. The preconditions for a “good food” are: price.  Using local food industry by-products as sources of antioxidants. through using vegetal material without economic potential. Doctoral theses topics are related to study the phytoadditives and the benefits of their inclusion in animal diets (2 PhD students from CO team) and studies on the properties of medicinal plants (3 PhD students from P3 team). functional foods thus obtained. nutrients. decreases the amount of waste stored.  Data that can be used in European databases concerning the food industry by-products that can be used as antioxidants in poultry nutrition. considered waste. and management of intellectual property rights The main project result and the dissemination strategy is summarised in the following table: 50 PN-II-PT-PCCA-2011-3 .

In order to be able to prove ownership of foreground. national exhibition Brochure Month 31 Business field (feed producers. The beneficiaries will define in the Consortium Agreement what IP is considered necessary with respect to the obligation to grant access rights. 18. seminar Business field (feed producers. The journal publication or other means of putting foreground in the public domain constitute appropriate alternatives. 51 PN-II-PT-PCCA-2011-3 . 29 Communications Month 29 Scientific events Participation at conferences. Internet. The process of protection and exploitation of the intellectual property will be regulated by the Consortium Agreement. taking into account the specificity of the project. as well as any intellectual property rights which are needed for carrying out the project or for using foreground.Result Newsletter Date Month 2 Target for dissemination National and international research community. the nature of the results concerned and the legitimate interests of the participants. 8. press release. The Background that we will consider represent the relevant information to the project held by the participants prior to their accession to the Consortium Agreement. poultry industry Development of a nutritional strategy to optimize feed recipes An established framework within the Consortium Agreement regulates the Backgroung and the Foregroung intellectual property rights (IPR). professional and patronal assossiations Professional events. 30 Member of the network. 34 Month 14. mailing. 26. symposium. 27. Contracting Authority Mainstream journals Web portal Obtaining relevant and original results Communications Month 25. all the participants will maintain documents showing the development of the generation of knowledge or results. wich are supervised by the ProjectCoordinator and the Project Administrator. 29 Feed producers. professional and patronal assossiations Strategy Web portal. Project progress report Articles Month 3. The Foreground resulting from the project belongs to that participant which generated it. professional si patronale assossiations) Information and awareness of consumers Pending patent application Month 18.

The company utilises an area of 35 hectares. and teas. Business case – only for Type 2 projects “Hofigal” is one of the famous brand names when it comes to homeopathic.14000. – all of which represent an integrated system. being characterised by the fact that they produce in their own greenhouses and agricultural lands the majority of the raw materials that they use. and teas conform to the international norms regarding the cultivation and harvesting of plants. Patent applications and expected use of foreground will be reported in the plan for the use and dissemination of foreground. cosmetics. and I. made in the form of pharmaceutical products. Quality Control. Regulatory Affairs. is a company that is specialised in the manufacture of exclusively natural products. “Hofigal” S. Logistics and Distribution. regardless of what stage they are in. being continually motivated towards a professional and personal perfection. UMF. complemented with those of other internationally circulating pharmacopoeias. and phytotherapeutic galenical products in Romania. they fulfil the requirements of the European Pharmacopoeia. these people carry out their activities covering a wide range of fields: Agriculture. Total Quality Management. as well as the rules for Good Manufacturing Practice. dietary supplements. plant processing). UB. With regard to the quality of the products. Administrative. Legal. Supply. ISO 9001. All of the stages of the process.A.22000 obtained from German TUV company .18000. Marketing. These new products will represent an important source of economic development and also will protect the animal food which influence the growth and the health of its. “Hofigal” is a share-held company comprising an exclusively Romanian private capital. The project is well integrated in the development strategy of company by the specific concerns it has: working feed based on indigenous raw materials rich in nutrients and antioxidants. Research and Development.Each participant will ensure that the foreground it owns is disseminated as swiftly as possible. gemoderivative. Production Planning. Hofigal. Approximately 299 employees work in the company. Production. Maintenance. any dissemination will be delayed until a decision about its possible protection has been made (through IPR). down to the finished product in the form of pharmaceutical products. 52 PN-II-PT-PCCA-2011-3 . Sales. The information in this application is hereby certified to be correct. However. cosmetics. New Therapies. Research results of the project: a new formulation adequate of variability feed ingredients. will be implemented at pilot and industrial scale by cooperation of partners: IBNA. from cultivation (pre-processing. USAMV. Human Resources. dietary supplements. and of the quality conditions pertaining to Hofigal philosophy.T.

11.Project leader. Last name.2011 53 PN-II-PT-PCCA-2011-3 . first name: CRISTE RODICA DIANA Signature: Date: 7.

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