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Analysis of Theobromine in Horse Urine

Analysis of Theobromine in Horse Urine

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Published by Sam Thornton
Analysis of Theobromine in Horse Urine
Analysis of Theobromine in Horse Urine

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Published by: Sam Thornton on Jul 03, 2013
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07/03/2013

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Analysis of Theobromine in Horse Urine

Abstract The purpose of the experiment was to determine the concentrations of theobromine in horse urine, and compare the concentrations to determine if any of the horses had been doped with caffeine. Three horses had been determined to be doped in this way; Sunny Side Up, When we were Young and Roman Gladiator. Introduction The aim of this experiment was to determine quantitatively the concentration of theobromine in horse urine to show if any of the horses had been doped with caffeine. Experimental procedure The usual safety precautions of good lab practice, gloves and goggles were followed at all times. The procedure in the lab manual was followed carefully. Discussion

Caffeine

Theobromine

The retention time for caffeine is 5.74s and the retention time for theobromine is 2.86s

The horses we suspect of being doped are “Sunny Side Up”, “When we were Young” and “Roman Gladiator” and they were found to have concentrations of theobromine equal to 4.008ppm, 3.684ppm and 1.5618ppm respectively.

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