3.2 Studying osmosis using an osmometer Problem statement: What substances can diffuse through a semi-permeable membrane? Hypothesis: Water molecules can diffuse through a semi-permeable membrane. Variables: Manipulating – Time Responding – Increase in level of sucrose solution Constant- Surrounding temperature, concentration of sucrose solution Materials and apparatus: 30% sucrose solution, distilled water, Visking tubing, cotton thread, retort stand, 25 cm capillary tube, ruler, marker pen, scissors, 250 ml beaker, syringe and stopwatch Procedure:

1. 2. 3. 4. 5. 6. 7. 8. 9.

(P1) Cut a Visking tubing about 8 cm long. (P1) Soak the Visking tubing in water for about 5 minutes to soften it. (P1) Tie tightly with one end of the Visking tubing to form a bag. (P1) Fill the Visking tubing with 30% of sucrose solution using a syringe. (P1, P2) Tie the other end of Visking tubing to the bottom of the capillary tube. (P1) Rinse the outer surface of the Visking tubing with distilled water. (P1, P5) Clamp vertically the capillary tube to the retort stand. (P1) Immerse the Visking tubing in a beaker filled with distilled water. (P1) Mark the initial level of the sucrose solution in the capillary tube with a marker pen at the beginning of the experiment. (P1, P3)

©Imelda ak Nyaun/S.M.K. Serian


10. Mark the level of sucrose solution every 10 minutes for duration of 40 minutes. Measure and record the level of the sucrose in capillary rube of each interval. (P3, P4) Result: Time (minute) 0 10 20 30 40 Increase in height of sucrose solution from the initial level (mm)

©Imelda ak Nyaun/S.M.K. Serian

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