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Fluorescence Microscopy made by Leica Microsystems
The time after one new development is the time before the next Today, biomedical research is inconceivable without continuous progress in fluorescence microscopy. New fluorescence technologies often initiated new discoveries and soon became standard applications, such as immunofluorescence, which is now a routine diagnostic technique. Further examples are confocal microscopy and live cell examinations with GFP. The invention of fluorescence microscopy ... In 1932, Leica designed the first epifluorescence microscope, whose technology is still used today. Then, in 1965, epifluorescence filter cubes were developed at Leica in cooperation with Professor Ploem; they were adopted by other manufacturers and have remained the generally accepted standard right up to the present day. As Leica is in constant contact with leading research scientists and laboratories all over the world, our instruments offer special customer benefits which we combine with innovative technologies and the quality of Leica optics. ... and the revolution in light microscopy The latest example of our pioneering spirit and probably one of the most fascinating developments ever is the high-resolution Leica TCS 4Pi microscope, which can image cell structures that were previously beyond the resolving power of conventional light microscopy. Together with Professor Stefan Hell, Director of the Max Planck Institute for Biophysical Chemistry in Göttingen, Leica Microsystems has developed a method which leads to a substantial reduction of focal volume. This improves the resolution along the optical axis three- to seven-fold, opening completely new horizons for research scientists.
Brilliant, Convenient, Practical
If only all decisions were so simple Despite the complexity of a fluorescence microscope, the criteria for its quality are crystal clear. There are three main aspects for assessing any fluorescence microscope:
Optics, i.e. the brilliance and wealth of contrast of the images
Practical orientation and application potential, i.e. a wide and flexible range of equipment Brilliance: a visible difference Leica microscopes have always been famous for their optics. And rightly so:
Ease of use, i.e. well thought-out operation concept
Leica images feature outstanding fluorescence, razor-sharp contours and breathtaking contrast. The finest structures are clearly visible even when the excitation intensity is attenuated; unique brilliance ensures top imaging precision. Ease of use: when jobs get harder, tools must get easier It may sound paradoxical, but the more difficult the scientific challenges, the easier the microscope should be to use. This is the only way to be able to concentrate fully on the subject of research and obtain quick results even in extremely time-consuming research series. Practical orientation: reproducibility and precision for all applications From climate chambers to sample manipulation to filter changers: all components interact in perfect harmony, guaranteeing simple work routines, reproducible images and, most importantly, the knowledge that you can rely a hundred percent on your results.
Answers To Every Question
Fluorescence stereomicroscopy and macroscopy: Select, sort and assess in 3D fluorescence. Our stereomicroscopes allow 3D imaging of specimens and therefore precise manipulation of fluorescing objects. The upright fluorescence macroscopes, on the other hand, offer parallaxfree precision and a free working distance that is long enough to enable viewing of specimens the size of a mouse.
Fluorescence microscopy: Our range of fluorescence microscopes is extremely wide. From the DM1000 standard routine microscope to the high-end research microscopes DM6000 and DM I6000, you’ll be sure to find the right system for any application: we offer an extensive choice of different cameras and software solutions. All the systems feature brilliant optics, so there’s no need to sacrifice high precision whatever the size of your budget.
Confocal microscopy: The essential characteristics of confocal microscopy are top image quality through optical sections and outstanding three-dimensional reconstruction of data sets. We offer many different configurations in this area of microscopy – including the world’s first filter-free Spectral Confocal Microscope.
Digital Photography and Data Analysis
An essential requirement for reliable data analysis is excellent optical imaging quality as guaranteed by our digital cameras. Choose between various models to suit your application needs. Besides actual image information, interest is increasingly focusing on the development of new experimental fluorescence techniques for exploring cell functions. These require powerful software solutions that are precisely matched to your specifications and perfectly tuned to our cameras and systems. Thus equipped, you can rely on smooth and safe data analysis and management for all your projects.
Image processing: In traditional fluorescence microscopy, images frequently seem slightly blurred due to the fact that information coming from outside the focal plane is included in the image. Image quality can be significantly enhanced by processing with mathematical algorithms (deconvolution).
Experimental fluorescence techniques: Fluorescence Recovery after Photobleaching (FRAP), Fluorescence Resonance Energy Transfer (FRET), Photoactivation, Fluorescence Liftetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), etc. enable the representation of molecular interactions and dynamics, answering questions on cell functions.
Data analysis: The software-supported quantitative evaluation of image data e.g. for FCS or FLIM round off the scientific information yield of the experiment.
Leica Image Manager: Intuitive ergonomic software solutions for professional image administration Complete modular concept ranging from basic to sophisticated requirements Sophisticated and flexible processing and analysis software for image analysis
Leica QWin: Solutions for quantitative microscopy Sophisticated and flexible software for image analysis Integrated solutions tailored for your application requirements Capabilities range from simple to automatic, multi-parameter measurements Optional modules for additional functionalities
Advanced fluorescence imaging techniques Powerful software and hardware solutions for FRET, FRAP, FLIP, FLIM, FCS, Photoactivation, Photoconversion Easy to use unique application wizards, guiding step by step through FRET and FRAP experiments Permanent application improvements and further innovation
Brilliant Leica optics – Innovation by experience Wide range of high NA objectives optimized for all kinds of fluorescence applications. Lowest autofluorescence for best signal to noise ratio Special objectives for oil, water, glycerol immersion, temperature adjustment, corrected from UV to Infrared
Complete equipment for environmental control Sophisticated accessories for heating, cooling, CO2, O2, pH control for all types of microscopes and systems Modern, state of the art functional and ergonomic design perfectly fitted to Leica/our products User-friendly and convenient to handle
Leica MZ16 FA: The world’s best fluorescence stereomicroscope Fully apochromatic 16:1 optic system, 920x magnification, 840 line pairs/mm resolution, 600 µm visible linewidth and 0.282 NA numerical aperture Patented TripleBeam® fluorescence technology for intensive fluorescence imaging Motorized functions: Filter changer, zoom, dual iris diaphragm, focus drive, UV shutter, digital display and automated control of multifluorescence experiments.
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Leica MacroFluo: The world’s only fluorescence macroscope Parallax-free imaging for high-precision analysis, measurements, digital and video documentation particularly with multifocus and deconvolution programs Choice of apochromatic 6.3:1 or 16:1 zoom Maximum macroscopic resolution for documentation of experiments on large living objects (mice): 340 Lp/mm with the 6.3:1 and 336 Lp/mm with the 16:1 zoom
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Leica DM Series: Setting new standards for upright microscopes Wide range of upright microscopes for routine and research applications in fluorescence microscopy Intuitive and ergonomic handling, one button changing of contrasting methods with automated adjustments of diaphragms, prisms and intensity values Brilliant fluorescence, up to 8 pixel shift-free filtercubes, Fluorescence Intensity Manager
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Leica DMI Series: Most sophisticated inverted microscopes, optimized for fluorescence Innovative new microscope design with up to 4 ports, fluorescence optimized optics and unique handling comfort Fast internal filter wheel for vibration free excitation of fluorochromes within milliseconds and excitation manager to balance different fluorochrome intensities Fluorescence Intensity Manager for attenuation of excitation light intensity to avoid photobleaching or stress in living cells
Leica DM LFSA: Unique automated fixed stage microscopes The ultimate solution for electrophysiology, patch clamp and infrared microscopy Apochromatic long working distance water immersion dip in UVI objectives corrected from UV to infrared (380–1000 nm) Highest precision focussing, slim design and stable set up for vibration-free working
Leica AS TP: New benchmark for micromanipulation The synergy of the combined know-how and experience of two leading suppliers in the field of micromanipulation Integration of microscope- and micromanipulator functions into one control element Efficient and comfortable working without touching the microscope Brilliant fluorescence for GFP-expressing transgenic organisms
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Leica AS LMD: Contact and contamination-free Laser microdissection Highest precision laser microdissection for collection and analysis of cells and area from tissues and cultured cells Simultaneous laser cutting and fluorescence imaging Powerful software for automated detection, dissection and collection of fluorescent stained cells
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Leica AS MDW: Life Cell Imaging in 6 dimensions Sophisticated integrated solution for z-stacking, multichannel fluorescence, time lapses with highest light efficiency and image acquisition speed. All state of the art components e.g. monochromator, fine focussing, high performance camera are perfectly synchronized via unique speed board technology Optimized environmental control allows the analysis of dynamic processes in living cells
Leica TCS SP2 AOBS: Revolutionary breakthrough – Filterfree spectral confocal microscope AOBS – the ultimate beamsplitter: freely programmable to any multichroic beamsplitter specification with up to 8 sharp selection bands The AOBS in combination with the spectral detector gives highest emission collection efficiency resulting in unmatched spatial and spectral resolution and brilliant image quality Easy and intuitive handling of hardware and Leica Confocal Software
Leica TCS SP2 RS: Ultrafast point scanning confocal microscope High speed resonant galvanometer driven point scanner with up to 8000 lines/second allows imaging of fast dynamic processes Uncompromised spatial resolution and excellent signal to noise ratio gives outstanding performance Simultaneous multi color imaging combined with transmitted light detection
Leica TCS SP2 MP: In-depth viewing Multiphoton microscopy is advantageous for the examination of thick specimens Excitation occurs only in the focal volume which leads to reduced phototoxicity and is therefore ideal for live cell imaging, in addition external detectors increase the fluorescence collection efficiency Available turnkey lasers are intuitive and easy to use
Leica TCS 4Pi: Entering the nanoworld – highest resolution microscopy First commercial 4Pi system, providing a stable platform with performance at theoretical limits Breaks the axial resolution barrier and provides new insights for structural biology of live and fixed specimens
Leica DFC FX: Digital camera range for all requirements in fluorescence microscopy Wide range of fluorescence digital cameras optimized for analysis and documentation Highest sensitivity: short exposure time for fast image acquisition and to prevent photobleaching Optimized signal to noise ratio using peltier cooling elements
Leica CW4000: Solutions for cytogenetic applications Advanced software modules for True High Resolution Karyotyping, FISH, CGH and MFISH Intelligent image handling in Leica CW4000 Karyotyping software, saving time and increasing productivity FISH Spot counting facility included in Leica CW4000 CytoFISH software Proven solutions for Comparative Genomic Hybridization (CGH) and Karyotyping (MFISH)
Leica FW4000: Fully integrated system for multidimensional widefield imaging Complete integration with Leica’s DM Microscope and Fluorescence Digital Camera range for fully automated image acquisition ‘Easy to use’ and intuitive interface Suitable for simple fluorescence image acquisition through to z stacking and time lapse experiments Additional modules for 3D Deconvolution and visualization for the ultimate in image resolution
Leica Microsystems’ mission is to be the world’s first-choice provider of innovative solutions to our customers’ needs for vision, measurement, lithography and analysis of microstructures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition.
The companies of the Leica Microsystems Group operate internationally in four business segments, where we rank with the market leaders.
• Microscopy Systems
Leica Microsystems – an international company with a strong network of customer services
Australia: Austria: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: People’s Rep. of China: Portugal: Singapore Spain: Sweden: Switzerland: United Kingdom: USA: Gladesville Vienna Richmond Hill/Ontario Herlev Rueil-Malmaison Bensheim Milan Tokyo Seoul Rijswijk Hong Kong Lisbon Barcelona Sollentuna Glattbrugg Milton Keynes Bannockburn/lllinois Tel. +61 2 9879 9700 Tel. +43 1 486 80 50 0 Tel. +1 905 762 2000 Tel. +45 4454 0101 Tel. +33 1 473 285 85 Tel. +49 6251 136 0 Tel. +39 0257 486.1 Tel. +81 3 5435 9600 Tel. +82 2 514 65 43 Tel. +31 70 4132 100 Tel. +852 2564 6699 Tel. +351 21 388 9112 Tel. +65 6779 7823 Tel. +34 93 494 95 30 Tel. +46 8 625 45 45 Tel. +41 1 809 34 34 Tel. +44 1908 246 246 Tel. +1 847 405 0123 Fax +61 2 9817 8358 Fax +43 1 486 80 50 30 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 473 285 86 Fax +49 6251 136 155 Fax +39 0257 40 3273 Fax +81 3 5435 9615 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 1 809 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164
• Specimen Preparation
We provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedical research and industrial quality assurance. Our product range includes instruments, systems and consumables for tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers.
• Medical Equipment
Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery.
and representatives of Leica Microsystems in more than 100 countries.
• Semiconductor Equipment
Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world.
Order nos. of the editions in: English 924 085 • German 924 084 • Part-No. 500-186 Printed on chlorine-free bleached paper. III/05/???/????
Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. With confocal laser technology and image analysis systems, we provide threedimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences.
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Leica Microsystems – the brand for outstanding products
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