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Pre Bromalain treated R1R1 pooled

Treated
cells
ate
date
26/1/09
22/1/09
test
Test
date
date
26/1/09
26/1/09

Anti-D 73/515
Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0

AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp1 4 4 2 2 1 0

reated cells washed in cellstab


AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp1 4 3 2 1 0 0

Pre bromalain treated pooled rr red


treated
cells Treated
date 26/1/09
26/1/09
tdatetest
26/01/09
Test
date
date
26/1/09
26/1/09
Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Sal 0 0 0 0 0 0
AHG 0 0 0 0 0 0
Pre Bromalain treated R1R1 pooled Treatedcells
date 26/1/09 Test date 26/1/09
Anti-D 86 i.u.
Saline card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 2 1 0 0 0
Exp 2 4 2 1 0 0 0
Exp 3 4 2 1 0 0 0

AHG card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 2 0 0
Exp 2 4 4 3 2 0 0
Exp 3 4 3 3 1 0 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled


Treatedcells
ate
date26/1/09
26/1/09
9 Test
test
Test
date
date
date
27/1/09
27/1/09
Anti-D lot 24311
Saline card
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 0 0
AHG card
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 3 3 2 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 0 0 0
Exp 3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test date 26/1/09
Anti-D Lot 32372B
Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 2 1 0 0
Exp 2 4 4 2 1 0 0
Exp 3 4 4 2 1 0 0

AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 4 2 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 4 2 1 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test date 26/1/09
Anti-D Lot 101
Saline card
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 4 2 0 0 0 0
Exp 2 4 2 1 0 0 0
Exp 3 4 2 1 0 0 0

AHG card
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 4 4 2 1 0 0
Exp 2 4 4 2 0 0 0
Exp 3 4 4 2 1 0 0
Untreated cells in cellstab
Standard AHG technique
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 2 2 1 0 0 0
Exp 2 3 2 0 0 0 0
Exp 3 3 2 0 0 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test date 26/1/09
Anti-D Patient Pool A
Saline card
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 3 1 0 0 0 0

AHG card
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 4 2 1 0 0 0
Exp 2 4 2 1 0 0 0
Exp 3 4 2 1 0 0 0

Untreated cells in cellstab


Standard AHG technique
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 2 1 0 0 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test date 27/1/09
Anti-D Lot 102
Saline card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 1 0 0 0
Exp 2 4 4 1 0 0 0
Exp 3 4 3 1 0 0 0

AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 2 1 0 0 0
Exp 2 3 2 1 0 0 0
Exp 3 3 2 0 0 0 0
Pre Bromalain treated R1R1 pooled
Treated
cells
date 26/1/09 Test date 27/1/09
Anti-D Lot 201
Saline card
Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600
Exp 1 2 0 0 0 0 0
Exp 2 2 0 0 0 0 0
Exp 3 2 0 0 0 0 0
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
3 0 0 0 0 0

AHG card
Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600
Exp 1 3 2 0 0 0 0
Exp 2 3 2 0 0 0 0
Exp 3 3 2 0 0 0 0
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
4 2 1 0 0 0
Untreated cells in cellstab
Standard AHG technique
Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 2 1 0 0 0 0
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
4 3 1 0 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test date 27/1/09
Anti-D Lot 202
Saline card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 0 0 0 0 0
Exp 2 2 0 0 0 0 0
Exp 3 2 0 0 0 0 0

AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 2 1* 0 0 0
Exp 2 4 2 1* 0 0 0
Exp 3 4 2 1* 0 0 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 2 1 0 0 0 0
Pre bromalain treated cells R1R1 pool treated date 26/1/09 test date 27/1/09

Anti-D 73/515
Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 3 1 0 0
Exp 2 4 3 3 1 0 0
Exp3 4 3 3 1 0 0

AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 1 0
Exp3 4 4 3 2 1 0

R1R1 cells in cellstab


Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test
2009-1-29
date 27/1/09
Anti-D Lot 103
Saline card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 3 2 1 0 0
Exp 2 3 3 2 1 0 0
Exp 3 3 2 2 1 0 0

AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 2 2 1 0
Exp 2 4 3 2 2 1 0
Exp 3 4 3 2 1 0 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 4 3 1 0 0
Exp 2 4 4 3 1 0 0
Exp 3 4 3 3 1 0 0

Pre Bromalain treated R1R1 pooled


Treated
cells
date 26/1/09 Test date 29/1/09
Anti-D Lot 203
Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 3 3 1 0

AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 4 3 2 0
Exp 2 4 4 4 2 2 0
Exp 3 4 4 3 2 1 0

Untreated cells in cellstab


Standard AHG technique
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 1 0
AHG Technique inital titre
R1R1 pooled cells
Dilution
Sample Neat 1:2 1:4 1:8 1:16 1:32 1:64 1:128 1:256
Lot 101 4 4 4 4 4 4 2 1 0
Pat. Pool A 2 1 0 0 0 0
Lot 102 4 4 4 4 4 4 4 3 2
Lot 201 4 4 4 4 4 4 4 4 3
Lot 202 4 4 4 4 4 4 3 2 1
Lot 103 4 4 4 4 4 4 4 4 3
Lot 203 4 4 4 4 4 4 4 4 4
1:512 1:1023 1:2046
0 0 0

1 0 0
2 1 0
0 0 0
3 2 1
4 3 3
Bromelain addition technique Pooled R1R1 cells Pool A

Anti-D 73/515
AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 0 0 0
Exp2 4 3 2 0 0 0
Exp3 4 3 2 0 0 0
Anti-D 86 i.u.
AHG card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 1 0 0 0 0
Exp2 4 1 0 0 0 0
Exp3 4 1 0 0 0 0
Anti-D lot 24311
AHG card
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 3 2 1 0 0
Exp2 4 3 2 1 0 0
Exp3 4 3 2 1 0 0
Anti-D Lot 32372B
AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0
Exp2 4 3 2 1 0 0
Exp3 4 3 2 1 0 0
Anti-D Lot 101
AHG card
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 3 2 0 0 0 0
Exp2 4 2 0 0 0 0
Exp3 4 2 0 0 0 0
Anti-D Patient Pool A
AHG card
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 2 1 0 0 0 0
Exp2 2 1 0 0 0 0
Exp3 2 1 0 0 0 0
Anti-D Lot 102
AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 2 1 0 0 0
Exp2 3 2 1 0 0 0
Exp3 3 2 1 0 0 0
Anti-D Lot 201
AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 1 0 0 0 0
Exp2 2 1 0 0 0 0
Exp3 2 1 0 0 0 0
Anti-D Lot 202
AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 1 0 0 0 0 0
Exp2 1 0 0 0 0 0
Exp3 1 0 0 0 0 0
Anti-D Lot 103
AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 3 1 0 0
Exp2 4 3 3 1 0 0
Exp3 4 3 3 1 0 0
Anti-D Lot 203
AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 1 0 0
Exp2 4 4 3 1 0 0
Exp3 4 4 3 1 0 0
Papain treated R1R1 pooled cells Saline cards

Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 1 0
Exp 2 4 3 3 2 1 1

Anti-D 86 i.u.
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 3 1 0 0 0
Exp 2 4 3 2 0 0 0

Anti-D lot 24311


Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 4 3 2 2 1
Exp 2 4 4 3 3 1 0

Anti-D Lot 32372B


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 2 1 0

Anti-D Lot 101


Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 4 4 3 1 1 0
Exp 2 4 3 2 1 0 0

Anti-D Patient Pool A


Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 4 3 1 0 0 0
Exp 2 4 2 1 0 0 0

Anti-D Lot 102


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 3 1 0 0
Exp 2 4 2 1 0 0 0

Anti-D Lot 201


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 4 3 1 0 0
Exp 2 4 3 2 0 0 0

Anti-D Lot 202


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 1 0 0 0 0
Exp 2 3 0 0 0 0 0

Anti-D Lot 103


Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 4 3 3 1 0
Exp 2 4 4 3 3 1 0

Anti-D Lot 203


Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 2 2 1 0
Exp 2 4 4 3 3 2 1

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